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Method for producing avilamycin by fermenting

A technology of avilamycin and seeds, which is applied in the field of fermentative production of avilamycin, can solve the problems of unstable fermentation results, cumbersome seed production process, easy degradation of strains, etc., so as to reduce the risk of bacterial contamination and improve biological activity High, the effect of promoting animal growth

Active Publication Date: 2013-08-07
江西兴鼎科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, avelamycin is mainly cultivated on a traditional slant. The inoculation ring is dug to inoculate the shake flask, and the shake flask is inoculated into the seed tank. The seed production process is cumbersome, the strain is easy to degrade, the fermentation result is unstable, and the stability is poor.

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  • Method for producing avilamycin by fermenting

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Effect test

Embodiment 1

[0038] A method for producing avelamycin by fermentation, the technical scheme is as follows:

[0039] (1) Preparation of slanted spores

[0040] The MCCC1A01659-produced Streptomyces viridans purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 1A01659, was inoculated into the slant medium, and the slant culture was carried out to obtain slant spores. The surface of the slant was full of off-white to slightly gray spores;

[0041] (2) Preparation of frozen seed solution:

[0042] a. Inoculate the above-mentioned slant spores into 100ml seed culture medium, the inoculum size is 0.2×10 7 1 spore, culture temperature 28 ℃, culture time 24h, obtain seed liquid, the solid matter in seed liquid accounts for 20%V / V of seed liquid total amount;

[0043] b. Add an equal volume mass fraction of 40% glycerol aqueous solution to the seed liquid obtained above, mix well to make a glycerin seed liquid, subpackage in cryopreservation tu...

Embodiment 2

[0051] A method for producing avelamycin by fermentation, the technical scheme is as follows:

[0052] (1) Preparation of slanted spores

[0053] The MCCC1A01659-produced Streptomyces viridans purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 1A01659, was inoculated into the slant medium, and the slant culture was carried out to obtain slant spores. The surface of the slant was full of off-white to slightly gray spores;

[0054] (2) Preparation of frozen seed solution:

[0055] a. Inoculate the above-mentioned slant spores into 100ml seed culture medium, the inoculum size is 0.5×10 7 1 spore, culture temperature 28 ℃, culture time 30h, obtain seed liquid, the solid matter in seed liquid accounts for 25%V / V of seed liquid total amount;

[0056] b. Add an equal volume mass fraction of 40% glycerol aqueous solution to the seed liquid obtained above, mix well to make a glycerin seed liquid, subpackage in cryopreservation tube...

Embodiment 3

[0064] A method for producing avelamycin by fermentation, the technical scheme is as follows:

[0065] (1) Preparation of slanted spores

[0066] The MCCC1A01659-produced Streptomyces viridans purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 1A01659, was inoculated into the slant medium, and the slant culture was carried out to obtain slant spores. The surface of the slant was full of off-white to slightly gray spores;

[0067] (2) Preparation of frozen seed solution:

[0068] a. Inoculate the above-mentioned slant spores into 100ml seed culture medium, the inoculum size is 0.8×10 7 1 spore, culture temperature 28 ℃, culture time 36h, obtain seed liquid, the solid matter in seed liquid accounts for 30%V / V of seed liquid total amount;

[0069] b. Add an equal volume mass fraction of 40% glycerol aqueous solution to the seed liquid obtained above, mix well to make a glycerin seed liquid, subpackage in cryopreservation tu...

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Abstract

The invention discloses a method for producing avilamycin by fermenting. The method includes steps of preparing streptomycesolivaceoviridis with inclined surface with spores, connecting spores on the inclined surface to a seed bottle to culture to obtain seed solution, preserving the seed solution in hydraulic hydrogen; inoculating the frozen seed solution to a seed culture medium to culture to obtain the seed solution; inoculating the seed solution to a fermenting culture medium to be subjected to fermenting culture by the inoculation quantity of 5wt%; and further treating the fermented liquid obtained after fermentation to obtain crystals of avilamycin. The method is easy to carry out, completed seed making process is omitted, degeneration is reduced, contamination risk is lowered, inoculation quantity is easy to control, repeatability is good, fermenting results are good, and production cost is low. Further, the fermenting effect is higher than that of the prior art, and the outputof avilamycin can be over 6500 microgram / microliter.

Description

technical field [0001] The invention relates to a method for fermenting and producing avelamycin, belonging to the fields of industrial biotechnology and fermentation engineering. Background technique [0002] Avelamycin, also known as bembimycin and amelamycin, is a new type of digestion enhancer or metabolic regulator, produced by Streptomyces aeruginosa ( Streptomyces viridocbromogene ) Orthosomycin (oligosaccharide) antibiotic dichloroisophyllinate produced by fermentation, inhibits protein synthesis by binding to bacterial ribosomes, and is a special protein synthesis inhibitor. Avelamycin is neutral, in the form of colorless needle-like crystals, with a melting point of 181-182°C, slightly soluble in water, and easily soluble in organic solvents such as acetone, propanol, ethyl acetate, benzene and ether. Avelamycin has more than ten components such as A, A', B, C, D1, D2, E, F, G, H, I, J, K, etc., wherein A component has the highest activity. [0003] Avilamycin ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/60C12R1/465
Inventor 韩风华李国栋邹君彪邓健黄苓
Owner 江西兴鼎科技有限公司
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