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The invention discloses a method for relieving salt injury of soybean seedlings by using exogenous soybean isoflavone. The method comprises the following steps of: (1) dissolving the soybean isoflavone by using dimethyl sulfoxide (DMSO) to prepare soybean isoflavone aqueous solution containing 1 percent of DMSO, wherein the concentration of the aqueous solution is not more than 1.0mg / L; (2) soaking soybean seeds into the soybean isoflavone aqueous solution in an incubator of 25 DEG C, putting the seeds on a germinating bed after the seeds are soaked for 10 hours, and performing light shaded germination acceleration; (3) sowing the germinated seeds into a porous plastic cup filled with quartz sand, putting the plastic cup into a transfer box filled with 1 / 2 Hoagland nutrient solution, performing light cultivation, and changing the nutrient solution once every 2 to 3 days; and (4) moving seedlings to a porous foam board when a first pair of main leaves is grown, cultivating the seedlings in a transfer box filled with 100 to 130mmol / L NaCl solution prepared by using 1 / 2 Hoagland nutrient solution, observing the growth condition of the seedlings, measuring the area of the leaves, taking samples of a root, a stem and a leaf after the seedlings are treated for 6 days, and respectively measuring the soybean isoflavone content of the samples by adopting a high performance liquid chromatography (HPLC) method. By the method, the salt tolerance of the soybean seedlings can be enhanced, the salt injury of the soybean seedling is effectively relieved, and the method has more obvious effect on the soybean material with salt tolerance.

The invention provides a fast cultivation method for strawberry biochemical callus. The method comprises the following steps: 1) cleaning and sterilizing raw materials; 2) primarily culturing: using a cutter for cutting 0.5mm stem tip growing point, inoculating into a 0.1mg / L 6-BA MS culture medium for culturing and growing clumpy buds; 3) sub-culture: adapting the clumpy buds into the MS culture medium containing 0.2mg / L 6-BA and 0.01mg / L NAA and sub-culturing; 4) rooting culture: cutting big buds from the clumpy buds, adapting to the MS culture medium containing 0.5mg / L IBA and 0.1% activated carbon for inducing rooting, adapting the residual callus into the culture medium in the step 3) and culturing; 5) transplanting domestication: taking out the rooting seedlings, cleaning the root culture medium, transferring into a hole plate filled with the medium in greenhouse and domesticating. According to the method provided by the invention, the seedling culturing time is short, the subculture speed is high and the cultivating cost is saved.