Method for relieving salt injury of soybean seedlings by using exogenous soybean isoflavone

Abstract

The invention discloses a method for relieving salt injury of soybean seedlings by using exogenous soybean isoflavone. The method comprises the following steps of: (1) dissolving the soybean isoflavone by using dimethyl sulfoxide (DMSO) to prepare soybean isoflavone aqueous solution containing 1 percent of DMSO, wherein the concentration of the aqueous solution is not more than 1.0mg / L; (2) soaking soybean seeds into the soybean isoflavone aqueous solution in an incubator of 25 DEG C, putting the seeds on a germinating bed after the seeds are soaked for 10 hours, and performing light shaded germination acceleration; (3) sowing the germinated seeds into a porous plastic cup filled with quartz sand, putting the plastic cup into a transfer box filled with 1 / 2 Hoagland nutrient solution, performing light cultivation, and changing the nutrient solution once every 2 to 3 days; and (4) moving seedlings to a porous foam board when a first pair of main leaves is grown, cultivating the seedlings in a transfer box filled with 100 to 130mmol / L NaCl solution prepared by using 1 / 2 Hoagland nutrient solution, observing the growth condition of the seedlings, measuring the area of the leaves, taking samples of a root, a stem and a leaf after the seedlings are treated for 6 days, and respectively measuring the soybean isoflavone content of the samples by adopting a high performance liquid chromatography (HPLC) method. By the method, the salt tolerance of the soybean seedlings can be enhanced, the salt injury of the soybean seedling is effectively relieved, and the method has more obvious effect on the soybean material with salt tolerance.

Description

technical field

[0001] The invention belongs to the new technology field of stress tolerance regulation of crops, and relates to a method for alleviating salt damage of soybean seedlings, in particular to a method for alleviating salt damage of soybean seedlings by using exogenous soybean isoflavones. Background technique

[0002] Soybean isoflavones (soybean isoflavones) is a class of active substances isolated from soybean seeds with a typical structure of isoflavones, and is one of the most attractive functional ingredients in soybeans. A large number of studies have shown that soybean isoflavones have important physiological functions such as preventing cancer, cardiovascular disease and osteoporosis, anti-oxidation and alleviating women's menopausal syndrome. One of (Song Bing et al., 2008).

[0003] Soybean isoflavone metabolism is the main secondary metabolic pathway in soybean and other legumes. Soybean isoflavones can play a variety of important ecological roles in...

Examples

Embodiment 1

[0032]Weigh an appropriate amount of daidzin and genistin, dissolve them with a small amount of DMSO, and finally prepare a daidzin solution and a genistin solution containing 1% (V / V) DMSO, with concentrations of 0.01 mg / L and 0.1 mg / L and 1.0 mg / L. Soybean seeds were taken, soaked with the above-mentioned different concentrations of daidzin solution, genistin solution and 1% (V / V) DMSO solution respectively, and germinated after 10 hours in an incubator at 25°C. Take out half of the seeds soaked in 1% (V / V) DMSO solution, and germinate with deionized water (Control), and the other half of the seeds are germinated with 100 mmol / L NaCl solution (NaCl alone), and other treatments are germinated with 100 mmol / L NaCl solution. L NaCl solution for germination. For each treatment, those with consistent germination were selected and sowed in perforated plastic cups filled with quartz sand. Except that the control was placed in the turnover box filled with 1 / 2 Hoagland nutrient so...

Embodiment 2

[0034] According to the results of Example 1, 0.01 mg / L daidzin solution and genistin solution were selected for seed soaking treatment, and the seeds of each material were treated with 1% (V / V) DMSO solution and 0.01 mg / L daidzin solution respectively. , 0.01 mg / L genistin solution in a 25°C incubator for 10 hours, and then put it on a germination bed to avoid light to accelerate germination. Select the ones with consistent germination and sow them in a perforated plastic cup filled with quartz sand. / 2 Hoagland nutrient solution turnover box, cultured under suitable light and temperature, and the treatment solution was replaced every 2~3 days. When the first pair of true leaves grow out, transplant the seedlings to a foam board with holes, and carry out hydroponics in a turnover box filled with 1 / 2 Hoagland nutrient solution. When the seedlings grew to the first compound leaf, the materials soaked in 1% DMSO solution were divided into two groups, one group was the control (C...

technical field

[0001] The invention belongs to the new technology field of stress tolerance regulation of crops, and relates to a method for alleviating salt damage of soybean seedlings, in particular to a method for alleviating salt damage of soybean seedlings by using exogenous soybean isoflavones. Background technique

[0002] Soybean isoflavones (soybean isoflavones) is a class of active substances isolated from soybean seeds with a typical structure of isoflavones, and is one of the most attractive functional ingredients in soybeans. A large number of studies have shown that soybean isoflavones have important physiological functions such as preventing cancer, cardiovascular disease and osteoporosis, anti-oxidation and alleviating women's menopausal syndrome. One of (Song Bing et al., 2008).

[0003] Soybean isoflavone metabolism is the main secondary metabolic pathway in soybean and other legumes. Soybean isoflavones can play a variety of important ecological roles in...

Examples

Embodiment 1

[0032]Weigh an appropriate amount of daidzin and genistin, dissolve them with a small amount of DMSO, and finally prepare a daidzin solution and a genistin solution containing 1% (V / V) DMSO, with concentrations of 0.01 mg / L and 0.1 mg / L and 1.0 mg / L. Soybean seeds were taken, soaked with the above-mentioned different concentrations of daidzin solution, genistin solution and 1% (V / V) DMSO solution respectively, and germinated after 10 hours in an incubator at 25°C. Take out half of the seeds soaked in 1% (V / V) DMSO solution, and germinate with deionized water (Control), and the other half of the seeds are germinated with 100 mmol / L NaCl solution (NaCl alone), and other treatments are germinated with 100 mmol / L NaCl solution. L NaCl solution for germination. For each treatment, those with consistent germination were selected and sowed in perforated plastic cups filled with quartz sand. Except that the control was placed in the turnover box filled with 1 / 2 Hoagland nutrient so...

Embodiment 2

[0034] According to the results of Example 1, 0.01 mg / L daidzin solution and genistin solution were selected for seed soaking treatment, and the seeds of each material were treated with 1% (V / V) DMSO solution and 0.01 mg / L daidzin solution respectively. , 0.01 mg / L genistin solution in a 25°C incubator for 10 hours, and then put it on a germination bed to avoid light to accelerate germination. Select the ones with consistent germination and sow them in a perforated plastic cup filled with quartz sand. / 2 Hoagland nutrient solution turnover box, cultured under suitable light and temperature, and the treatment solution was replaced every 2~3 days. When the first pair of true leaves grow out, transplant the seedlings to a foam board with holes, and carry out hydroponics in a turnover box filled with 1 / 2 Hoagland nutrient solution. When the seedlings grew to the first compound leaf, the materials soaked in 1% DMSO solution were divided into two groups, one group was the control (C...

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