Fast cultivation method for strawberry biochemical callus

A technology of callus and culturing method, applied in the field of agricultural seedling raising, can solve the problems of long culturing time, slow proliferation rate, slow proliferation rate of strawberry, etc., and achieves the effect of saving tissue culture room space and ensuring filling time.

Inactive Publication Date: 2017-12-29
合肥华典生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Compared with the method of direct induction, Qiao Yushan et al. have a higher induction rate (37.5%) in the induction of adventitious buds through the callus approach. However, their technology still has the problems of slow proliferation and long culture time.
[0007] Therefore, this area urgently needs a kind of strawberry fast cultivation method, can solve the problem that strawberry multiplication speed is slow, the cultivation time is long

Method used

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  • Fast cultivation method for strawberry biochemical callus
  • Fast cultivation method for strawberry biochemical callus
  • Fast cultivation method for strawberry biochemical callus

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specific Embodiment approach

[0036] Strawberry mesenchymal callus rapid culture method provided by the invention, the specific embodiment comprises the following steps:

[0037] 1) Cleaning and sterilizing materials: select the tip of the stolon, cut off the front end about 3-4cm, wash it with washing spirit for 5 minutes, put it in a beaker and wrap it with gauze, and rinse it with running water until there is no obvious stain on the surface. The stem tip after washing is soaked in 75% alcohol for 30 seconds, after pouring off the alcohol, soak 20 minutes with 0.1% mg / L mercuric chloride solution, rinse 3 times with sterile water after draining.

[0038] 2) Primary culture: Use pointed steel tweezers to peel off the multi-layered young leaves, wrappers and petioles from the sterilized stem tip, expose the growth point, cut 0.5mm with a knife, and inoculate it into the primary culture medium; Primary culture There are three combinations of MS+6-BA0.1mg / L, MS+6-BA0.5mg / L and MS+6-BA 1 mg / L.

[0039] After...

Embodiment 1

[0053] The steps of strawberry callus rapid culture method are:

[0054] 1) Cleaning and sterilizing materials: select the tips of stolons in autumn, cut off the front end about 3-4cm, rub and wash with washing spirit for 5 minutes, put them in a beaker and wrap them with gauze, and rinse them with running water until there are no obvious stains on the surface . The shoot tip after washing is soaked in 75% alcohol for 30s, after pouring off the alcohol, soak 20 minutes with 0.1% mg / L mercuric chloride solution, rinse 3 times with sterile water after being filtered.

[0055] 2) Primary culture: Use pointed steel tweezers to peel off the multi-layered young leaves, wrappers, and petioles from the sterilized stem tip, expose the growth point, cut 0.5mm with a knife, and inoculate it into the primary culture medium. The base combination is: MS+6-BA 0.1mg / L. After cultivating for 30 days, the number of sprouts induced was between 3-5, and each sprout was 3-5mm long.

[0056] 3) ...

Embodiment 2

[0060] Other steps in this example are the same as in Example 1, the only difference is that in step 3), the combination of induction medium is: MS+6-BA 0.5 mg / L+NAA 0.1 mg / L.

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Abstract

The invention provides a fast cultivation method for strawberry biochemical callus. The method comprises the following steps: 1) cleaning and sterilizing raw materials; 2) primarily culturing: using a cutter for cutting 0.5mm stem tip growing point, inoculating into a 0.1mg / L 6-BA MS culture medium for culturing and growing clumpy buds; 3) sub-culture: adapting the clumpy buds into the MS culture medium containing 0.2mg / L 6-BA and 0.01mg / L NAA and sub-culturing; 4) rooting culture: cutting big buds from the clumpy buds, adapting to the MS culture medium containing 0.5mg / L IBA and 0.1% activated carbon for inducing rooting, adapting the residual callus into the culture medium in the step 3) and culturing; 5) transplanting domestication: taking out the rooting seedlings, cleaning the root culture medium, transferring into a hole plate filled with the medium in greenhouse and domesticating. According to the method provided by the invention, the seedling culturing time is short, the subculture speed is high and the cultivating cost is saved.

Description

technical field [0001] The invention belongs to the technical field of agricultural seedling cultivation, and in particular relates to a rapid culture method for strawberry meristematic callus. Background technique [0002] With the continuous improvement of Chinese people's living standards, the demand for fresh fruits is also increasing year by year. Among the rich fruit varieties, strawberries occupy a large position with their sweet taste. Strawberry cultivation usually uses traditional stolon propagation and branch propagation methods. After many years of cultivation, it is infected by a variety of viruses, resulting in the degeneration of the variety: weak growth, reduced yield, reduced resistance, and reduced fruit quality and commodity traits. [0003] In order to solve the above-mentioned situation, in vitro rapid propagation and plant detoxification technology are cited in the production of strawberry seedlings. The plant detoxification technology is to enter and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何荣荣
Owner 合肥华典生物科技股份有限公司
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