The invention discloses a seedling breeding method rapid rooting of hylocereus undatus cutting. Treatment on hylocereus undatus cutting seedlings and soaking treatment by using a potassium permanganate solution exert an effect of sterilization; root rot is eradicated and hylocereus undatus rooting is promoted; the seedling breeding medium consists of sand, soil, organic fertilizer, perlite and decomposed sugarcane, can provide enough organic matters, has loose soil, does not harden easily, can provide nutrients in a seedling period, and promotes the growth of seedling roots; in the seedling period, large amount of water for watering is needed, and a phenomenon of root rot which is caused by water ponding formed by the water does not occur easily; reasonable fertilizer application is performed according to different fertilizer needed in the growing period of the cutting seedlings; the adopted water and fertilizer have rich nutrients, have slow and lasting fertilizer effects, do not harm the roots, are beneficial for the growth of the cutting seedlings, shorten the time for seedling breeding, and enable high survival rate. The seedling breeding method provided by the invention is simple, has low costs, and can greatly increase the speed of seedling breeding; the breeding speed is fast; spindling of the seedlings does not occur easily; the disease and pet resistance is good; the fruit setting rate is high; and the method has high economic benefits and is worth popularizing.