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A kind of aseptic rapid propagation method of black pine

A fast and black pine technology, which is applied in the field of plant tissue culture research to achieve the effect of accelerating the speed of seedling cultivation

Inactive Publication Date: 2019-03-08
SHANDONG UNIV
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Problems solved by technology

At present, the successful induction of regenerated seedlings of black pine all uses zygotic embryos as explants, directly induces the production of adventitious buds from zygotic embryos or induces embryogenic callus to form somatic embryos, and then develops to form sterile seedlings. Technical report on callus proliferation and stem-bud differentiation to form regenerated shoots

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  • A kind of aseptic rapid propagation method of black pine

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Embodiment Construction

[0011] A kind of aseptic rapid propagation method of black pine is carried out through the following steps:

[0012] 1. Selection of explants

[0013] Collect young young pine branches from the top of black pine trees less than 7 years old in spring, pull out the pine needles on them, rinse them with running water, and carry out disinfection treatment in an ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, wash with sterile water for 2- 3 times, and then sterilized with 20% hydrogen peroxide for 17-18 minutes, shaking from time to time, washing with sterile water 2-3 times, to obtain sterile pine needles;

[0014] 2. Induction culture of callus

[0015] Cut off 1 cm long fragments from the base of sterile pine needles under sterile conditions, inoculate them in induction medium, and cultivate them at 25°C in the dark. The hormone dosage of induction medium was screened by two-factor design method: MS medium was used as the basic medium, pH=6.4, and 30 g / L suc...

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Abstract

The present invention relates to a kind of aseptic rapid propagation method of black pine, the steps are as follows: (1) take black pine needle leaf base segment in spring as explant; (2) inoculate in MS culture medium+6-BA 2mg / L+NAA 0.05 mg / L to induce callus; (3) inoculate the callus on 1 / 2MS medium+NAA 0.05mg / L+PSK 0.001mg / L to realize its massive proliferation; (4) inoculate the callus Induce adventitious bud differentiation on 1 / 4MS medium+NAA 0.01mg / L+PSK 0.002mg / L; (5) Cut out the single bud and inoculate it in 1 / 2MS medium+NAA0.25mg / L+IBA0.25mg / L Induce rooting on L; (6) transplant the rooted small green seedlings to the matrix whose formula is 1 / 4 fine sand + 1 / 4 vermiculite + 1 / 2 humus after hardening for further growth. The invention proliferates the callus in a large amount in a short period of time, and then differentiates the callus to form regenerated shoots, so as to realize seed preservation and mass propagation of excellent black pine individuals.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and the invention relates to a method for rapid aseptic propagation of black pine. Background technique [0002] Black pine (Pinus thunbergii) originated in Japan and was introduced to China in the 1920s. Because of its characteristics of drought resistance, resistance to sea wind and sea monsters, resistance to pine scale and pine caterpillar and other diseases and insect pests, and beautiful tree shape, it has become a coastal defense in the east of my country. It is an irreplaceable dominant species in forest construction, and it is cultivated from Dalian in the north, Lvshun in the south to the coastal areas of Taiwan and some inland areas. When it was first introduced, black pine had the characteristics of fast growth and strong resistance to diseases and insect pests. However, survey data in recent years have shown that black pine is superior in terms of vitality, growth speed, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 郑凤英刘雪芹梁中贵
Owner SHANDONG UNIV
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