105 results about "Pinus thunbergii" patented technology

The invention relates to the technical field of traditional Chinese medicine planting, in particular to a method for artificially planting parispolyphylla. The method includes the steps: (1), land selection and preparation; (2), seed selection and treatment; (3), seeding and seedling growing; (4), transplanting; (5), field management; (6), harvesting. Seedling growing is performed in a pine forest, and environment in the pine forest is more similar to wild environment, so that high adaptability and high survival rate of seedlings can be realized, and medicinal value of wild medicinal materials can be retained as much as possible; profit can be acquired in short time after artificial planting, so that planting investment risk is lowered. The method has the advantages of simple operation, easiness in popularization, high profit, low risk and the like, a novel optimized scheme is provided for artificial large-scale planting of parispolyphylla, and the method has high economic and social benefit.

The invention discloses a pinus thunbergii cell embryogenesis and plant regeneration method. Embryogenic calluses are induced from immature zygotic embryos; the embryogenic calluses are maintained, proliferated and matured to obtain somatic embryos; the somatic embryos are germinated to obtain regenerated plants; and finally the regenerated plants are transplanted. Due to the adoption of the pinus thunbergii cell embryogenesis and plant regeneration method, high reproduction rate of the pinus thunbergii plant is achived. After the embryogenic calluses are cultured for 70 days in a maturing culturing medium, 144-216 mature somatic embryos can be obtained in each culture utensil on average; the somatic embryo germination rate is 63.8%; and the plant transformation rate is 43.5%. Therefore, a large number of somatic embryo seedlings can be obtained every year, thus a foundation is laid for the pinus thunbergii cell embryogenesis and the regenerated plant bath production, and the current problem of afforestation seedling shortage is solved. The pinus thunbergii cell embryogenesis and plant regeneration method has very good practicality.

The invention discloses a cultivation substrate for artificially cultivating dendrobium nobile. The cultivation substrate is made of bryozoatum. The substrate of a cultivation bed for cultivating the dendrobium nobile is divided into three flatly laid layers from bottom to top, the first layer of the cultivation substrate comprises bryozoatum particles with the diameters ranging from 2.5cm to 4cm, the thickness of the first layer ranges from 3cm to 4cm, the second layer of the cultivation substrate comprises bryozoatum particles with the diameters ranging from 0.7cm to 1.5cm, the thickness of the second layer ranges from 3cm to 4cm, the third layer of the cultivation substrate comprises pine bark or alder bark, the diameter of the pine bark or the alder bark ranges from 2cm to 3cm, the laying thickness of the cultivation substrate ranges from 1cm to 2cm, healthy and strong dendrobium nobile seedlings with the heights ranging from 3cm to 8cm are cultivated in the substrate according to certain spacing in the rows, the certain spacing in the rows is 5-10X10-15cm, and the dendrobium nobile can be harvested after 11 months. The cultivation substrate has the advantages that the problem of difficulty in selecting substrates for dendrobium nobile cultivation procedures can be effectively solved, materials of the cultivation substrate are easily available, the cultivation substrate is low in cost, is not easy to decay and is good in air permeability and water permeability, high in water holding capacity and fertilizer conserving capacity, and favorable for healthy growth of the dendrobium nobile, and the dendrobium nobile is high in survival rate.

The invention relates to the technical field of air purification materials and manufacturing, and particularly provides a plant negative oxygen ion air purifying agent. The plant negative oxygen ion air purifying agent is prepared by mixing a pinus thunbergii extract, a cactus extract, tourmaline powder, monazite powder, nano titanium dioxide and a dispersing agent step by step. When the pinus thunbergii extract and the cactus extract are used during preparation, a plurality of modes such as superfine grinding, high-pressure homogenization, subcritical extraction, supercritical extraction andthe like are adopted to decompose macromolecular substances into micromolecular substances, so that the macromolecular substances have higher biological effects; then the tourmaline powder and the monazite powder are combined to rapidly induce the pinus thunbergii extract and the cactus extract to further release negative oxygen ions, and a larger effect is exerted on the aspect of air purification; meanwhile, the radiation effect of ore powder such as the tourmaline powder and the like can be reduced, so that the safety is higher; and on the other hand, the nano titanium dioxide can decomposeformaldehyde in the air, and the air can be purified fundamentally.

The invention provides a preparation method of cultivating substitute of lucid ganoderma, and relates to cultivating substitute of edible mushrooms. The cultivating substitute is prepared by the components which are mixture of pine, cedar, rotten wood, miscanthus sinensis, brans and bean dregs. The preparation method comprises the following steps: crushing the mixture of rotten wood, brans and bean dregs and the miscanthus sinensis, respectively; weighing the crushed materials in mass percent as requirement, and uniformly mixing; uniformly mixing the uniformly mixed material and water, and then fermenting; adding processed sawdust or sawing chips of pine and cedar to uniformly mix, and then uniformly mixing the mixture and water; and finally bagging and sterilizing the uniformly mixed mixture, thus obtaining the cultivating substitute of lucid ganoderma. According to the technical scheme mentioned above, the required raw materials for preparing the cultivating substitute are easy to obtain, and low in cost; and moreover, the lucid ganoderma cultivated by the cultivating substitute is fragrant, bitter in taste, and has high content of triterpenoids ganoderic acid, as well as high value in use.

The invention discloses a ganoderma tsugae culture material for cultivation in Northeast. The culture material comprises a first-grade ganoderma tsugae strain culture material and a second-grade ganoderma tsugae strain culture material, wherein the first-grade ganoderma tsugae strain culture material is prepared from the following components in parts by weight: 150-250 parts of potato, 10-25 parts of glucose, 14-25 parts of agar powder, 2-4 parts of monopotassium phosphate, 1-2 parts of magnesium sulfate, 0.5-1.5 parts of peptone and 1000 parts of water; the second-grade ganoderma tsugae strain culture material is added with broad leaf wood bits and pine wood bits, so that an obvious promotion effect on the growth of second-grade hypha of the ganoderma tsugae is achieved, the growth coarse of the ganoderma tsugae is quickened, the growth period of the ganoderma tsugae is shortened, the yield of the ganoderma tsugae is effectively increased, and the property of the ganoderma tsugae for resisting external adverse environment is improved, so that the nutritive value of the grown ganoderma tsugae is remarkably increased.

The invention discloses a wolfiporia cocos planting method and belongs to the technical field of planting of polyporaceae. The wolfiporia cocos planting method specifically includes steps of selecting a culture medium for wolfiporia cocos pulp in the size same as wheat kernels into PDA, culturing to obtain wolfiporia cocos mycelium and inoculating wolfiporia cocos mycelium into a stock culture medium to obtain wolfiporia cocos mycelium leads; cultivating plump and strong wolfiporia cocos strains by preparing niblet and fruit branch cultivating compost, annularly excavating grooves in pine stems, and inoculating the wolfiporia cocos strains into the peripheries of the grooves, fixing a bacterium bag by kelp, culturing by earth, reasonably sterilizing, killing insects, adjusting soil temperature and humidity, checking strain conditions periodically, and harvesting wolfiporia cocos in time. The wolfiporia cocos planting method is simple in operation, and biological efficiency is improved by using high salt of the kelp. Meanwhile, more sufficient nutrition is provided for growth of wolfiporia cocos, and large, high-yield and good-quality wolfiporia cocos are obtained.

The invention discloses a method for preparing a biological culture material by grinding and stirring before insulated fermentation. The method comprises steps as follows: S1, preparing the biological culture material: a material A contains cottonseed hull, bagasse, distiller's grains, rice bran, pine wood chips, soybean meal, palm kernel shells, decomposed pig manure, sucrose, light calcium carbonate, calcium superphosphate, potassium dihydrogen phosphate, magnesium sulfate and vitamin B; a material B contains spica prunellae slag, coconut shell powder, walnut shell powder, chestnut bracteal shell powder, lotus shell powder, banana stems and leaves, honeysuckle stems and leaves, radix angelicae dahuricae stalks, ginkgo leaves, garlic stalks, cassava stem bits, spartina anglica powder, ageratina adenophora, chrysopogon zizanioides, dicranopteris linearis, pennisetum purpureum, poplar leaves, ganoderma lucidum fruiting bodies, apple pomace, mushroom bran, xylose cinder and cyanobacteria; S2, the biological culture material prepared in the S1 is placed in a grinding and stirring device. A device for grinding and stirring before insulated fermentation is adopted for treatment, so that nutrients in a biological culture solution are easier to absorb, and the nutrients are richer.

The invention relates to a tea garden organic composite traditional Chinese medicine desinsectization medicine bag, which is prepared from a compounding agent and traditional Chinese herbal medicine according to the weight ratio of (4 to 8):(2 to 6). The traditional Chinese herbal medicine is prepared from the following ingredients in parts by weight: 10 parts of sandalwood tree leaves, 20 parts of southernwood, 20 parts of wormwood, 10 parts of phoenix tree bark, 5 parts of eucomrnia ulmoides oliv., 10 parts of pepper stems, 10 parts of garlic stems, 15 parts of willow leaves and 10 parts of pine needles; the compounding agent is prepared from the following ingredients in parts by weight: 10 parts of rice hull, 20 parts of camellia oleifera abel peel, 10 parts of pine sawdust, 10 parts of willow saw dust, 5 parts of parasol tree sawdust, 10 parts of yellow cinnamon sawdust, 10 parts of Chinese toon saw dust, 5 parts of wild arbor tree branch sawdust and 20 parts of tabia. The tea garden organic composite traditional Chinese medicine desinsectization medicine bag has the advantages that the raw materials can be easily obtained; the preparation is convenient; economy is realized; the time is reduced; a good prevention and control effect can be achieved on tea diseases and pests; the effect taking speed is high; the effect duration is long; the limitation of common use chemical medicine is avoided; the insect prevention and desinsectization effects are achieved; the original ecology quality is also ensured; the medicine bag conforms to the social green and environment-friendly trend.

Nitrogen is one of the principal factors limiting vegetative production. The present invention has improved the nitrogen metabolism in Poplar by integrating a transgene constitutively expressing a pine glutamine synthetase into the plan genome. The resulting transgenic trees exhibit higher growth rates, protein and chlorophyll contents, and leaf area than equivalent untransformed trees. It is contemplated that this approach to nitrogen improvement will be equally successful for all woody perennials. Provided with the invention is an expression casette, a vector, and a method for increase glutamine synthetase activity in woody perennials, as well as transgenic woody perennials with enhanced nitrogen metabolism and accompanying phenotype.

A culture medium of dendrobium candidum is composed of pine bark, bagasse and camellia oleifera bran, and is fermented with an EM strain. The culture medium composed of pine bark, bagasse and camellia oleifera bran is very suitable for growth of dendrobium candidum. Without addition of a chemical fertilizer, yield of dendrobium candidum can be guaranteed. Flaky and blocky pine bark is slowly converted into subsequent nutrients, thus forming a long-acting and balanced nutrient supply system. Meanwhile, blocky substances are distributed in the medium, thus making the medium to be loose and being beneficial to root system growth of dendrobium candidum. Camellia oleifera dregs have high fertilizer efficiency and are a natural plant-source ecological pesticide with advantages of no pollution, no residual toxicity, tolerance to storage, durability and long pesticide effect. With the application of camellia oleifera dregs, fertilizer efficiency of the culture medium is greatly enhanced, long-acting insect prevention of the culture medium can be realized, the culture medium has no pollution or residual toxicity, and a dendrobium candidum product with high yield and high quality can be cultured.

The invention belongs to the technical field of seed reproduction, in particular to a Bletilla striata seed direct seeding natural rapid reproduction seedling method.The method comprises the following steps that 1, ripe Bletilla striata capsules are sieved by a 120 mesh sample sieve for use after being dried in the sun; 2, a seed germination culture medium solution is prepared, 0.5-10 mg of NAA, 0.3-0.8 g of activated carbon, 100-150 g of potato juice with the concentration of 10%-15% by weight and 20-30 g of sugar are added to each liter of KC culture medium, and the pH value is adjusted to be 5.8-6.0; 3, 20 g of Bletilla striata seeds are placed into each bottle of nutrient solution to be soaked for 36-38 hours, and then green seed buds emerging by 0.2-0.5 mm are obtained by cultivation; 4, seedling is performed, the seedlings are moved out of a greenhouse after 3 or more leaves grow on the seedlings and are directly cultivated in a pine forest.

The invention discloses a planting method for increasing the bare-rooted seedling afforestation survival rate with water-saving irrigation. The planting method comprises the following steps: I, selecting tree species: selecting any one of the trees species pinus thunbergii, platycladus orientalis, elm, robinia pseudoacacia, cotinus coggygria and fraxinus chinensis; II, digging a planting hole: confirming the shape and the specification of the planting hole according to the type of the afforestation land and the specification of a seedling, wherein the planting hole generally takes the shape of a circular truncated cone in a plain area, the specification of the planting hole can be slightly large in a non-salt and alkali area, the diameter of the upper part of the planting hole is 60-80 cm, the diameter of the lower part of the planting hole is 40-60 cm, and the depth of the planting hole is 60-80 cm, and in a plain salt and alkali area, the diameter of the upper part of the planting hole is 50-60 cm, the diameter of the lower part of the planting hole is 40-50 cm, and the depth of the planting hole is 50-60 cm. By adoption of the planting method, limited moisture can be concentrated around the root system of the seedling and is not easy to evaporate or diffuse, water supply to the root system of the seedling in the key survival period can be assisted, sucking of weeds to moisture and nutrient in soil can be reduced, an ideal small environment is provided for survival and growth of seedling root systems, the afforestation survival rate can be ensured, and the problems that the afforestation irrigation cost is high and the afforestation survival rate is low in a water-deficient area are solved.

The invention belongs to the technical field of edible fungi cultivation, and particularly relates to a method for improving mycorrhiza seedling breeding of Tricholoma matsutake and application of themethod. The method comprises the steps of: performing separating and screening and identifying to obtain a Tricholoma matsutake strain YN1 which forms a symbiotic relationship with the rhizosphere ofa pine and has a preservation number of CCTCC NO:M2017719 by using a temperature difference method; adopting a large plate with a diameter of 14.5 cm as a mycorrhization container, adopting pine seeds as a test material, inoculating aseptic-germination pine seedlings and the Tricholoma matsutake strain YN1 to the plate on which cellophane is laid, fixing the pine seedlings by using a sterile barbed wire for cocultivation, performing stationary cultivation in an illumination incubator for 2-3 months, and observing an obvious Tricholoma matsutake mycorrhizal structure under a stereo microscope;and taking the Tricholoma matsutake mycorrhizal structure one year after transplantation is conducted, extracting mycorrhizal genomic DNA, and performing ITS molecule identification to obtain gel images of single bands. After comparison with NCBI, it is determined that the single bands are Tricholoma matsutake, and the accuracy of mycorrhization identification is 100%.

The invention discloses a novel material for improvement of soil for planting blueberries and a use method of the novel material. A novel material is provided for the soil improvement for a blueberry plantation the district in the south of the Yangtze river and can be used for replacing peat, so that the soil improvement cost is lowered. The novel material is characterized in that smashing and fermentation of pine barks and improvement on the soil are included.

The invention discloses a turpentine collecting masson pine bark automatically scraping and grooving machine for natural resin preparation. The turpentine collecting masson pine bark automatically scraping and grooving machine for natural resin preparation comprises a chassis, the bottom of the chassis is provided with electrically driven wheels, the top of the chassis is provided with a singlechip microcomputer, a power supply, an adjusting mechanism and a cleaning agent storage tank. The turpentine collecting masson pine bark automatically scraping and grooving machine for natural resin preparation has the advantages that the singlechip microcomputer drives the electrically driven wheels to work to move the chassis to a working area, so that external moving equipment can saved, and further trouble for mounting the turpentine collecting masson pine bark automatically scraping and grooving machine for natural resin preparation onto the moving equipment can be saved; the singlechip microcomputer controls a mechanical arm to work, the moving end of the mechanical arm drives a mounting frame to move and further to move a scraper to a position to be scraped, and the moving actions of the scrapes include extension and retraction, lifting and rotation; the turpentine collecting masson pine bark automatically scraping and grooving machine for natural resin preparation is high in flexibility and adjusting capability and convenient to operate.

The invention discloses a local cock breeding method. The local cock breeding method comprises the following steps: feeding cocks in a pine forest; opening grooves in pines and putting pine oil storage bags for receiving pine oil flowing out from the grooves under the grooves, wherein the cocks in the pine forest eat pine oil in the pine oil storage bags, and pine needles and pine leaves which fall off from the pines; and putting corns, rice husks and water into the pine forest for the cocks to eat. The cocks are bred in the pine forest, and eat the pine oil flowing out from the pines and the pine needles and pine leaves which fall off from the pines; and chicken has a high nutritive value and relatively good meat quality, and the cocks does not easily have diseases.

The invention relates to a fertilizer preparation method and in particular relates to a preparation method of a dendrobium officinale planting fertilizer. The dendrobium officinale planting fertilizer is prepared through taking pine barks and soybean cakes as raw materials, and mixing the fermented soybean cakes with the pine barks. The preparation method is reasonable in process, simple to operate and short in process period; the planting fertilizer produced by the preparation method can be used for greatly shortening the growth period of dendrobium officinale; meanwhile, pollution-free ecological planting can be realized, and the high-quality dendrobium officinale can be cultivated; the medicinal value of the dendrobium officinale is improved and planting cost is reduced; the fertilizer does not need to be applied in a planting process, the healthy growth of the dendrobium officinale is facilitated and the survival rate of the dendrobium officinale is improved.

The invention relates to a pesticide for pine wood nematode of masson pine. The pesticide comprises Sophora alopecuroides L., Veratrum nigrum L., trifoliate jewelvine root or stem, Tagetes erecta L., Sichuan chinaberry, virgate wormwood, stemona tuber, bitter bamboo and blackberry lily rhizome. The pesticide is prepared by weighing the above-mentioned components in proportion, crushing the components, adding the crushed components into ethanol, carrying out extraction, extracting herb residue with ethanol again, mixing herb soups collected after two extraction operations, subjecting the obtained mixture to pressure-reduced evaporation so as to obtain a viscous material and diluting the viscous material. Effective ingredients extracted from the components cooperate with each other, so the pesticide can effectively kill pine wood nematode and Monochamus alternatus Hope, controls propagation mediums of pine wood nematode, cures attacked pines, does not incur drug resistance and poses small influence to environment and ecology.

The invention discloses a pine bark-biochar matrix for culture of Dendrobium officinale. The pine bark-biochar matrix is prepared by mixing the following components in mass percentage: 56-67% of pine bark, 24-28% of peat and 5-20% of biochar. The pine bark-biochar matrix can substantially improve the contents of microelements necessary to a human body such as Zn, Co and the like in the Dendrobium officinale, can remarkably improve the polysaccharide content of the Dendrobium officinale, facilitates adsorption of potassium, and obviously improves the appearance and quality of the cultivated Dendrobium officinale.

The invention discloses a manufacture method of substrates for improving the alkaloid content of dendrobium huoshanense. The method includes the steps: (1) crushing and mixing pine wood chips, silkworm excrement and the like, placing mixture into a cooking layer to cook the mixture to obtain cooked mixture, mixing and cooking peat soil and diatomite to obtain mixed soil; (2) mixing perlite and granite particles, and leading in ammonia and nitrogen to calcine mixture to obtain modified mixed rock particles; (3) stirring and mixing the cooked mixture, the mixed soil and vegetated substrates, andspraying fungicide 1 solution in stirring to ferment mixture in a fermentation tank to primary fermentation mixture; (4) sterilizing the primary fermentation mixture at high temperature, performing aerobic fermentation in fungicide 2 solution, drying and sterilizing mixture to obtain secondary fermentation mixture; (5) stirring and mixing the secondary fermentation mixture, modified mixed rock particles and fungus powder charcoal layer to prepare dendrobium huoshanense planting substrates.

The invention discloses a Pinus thunbergii Parl rejuvenation substrate. The Pinus thunbergii Parl rejuvenation substrate is prepared from pitchstone, perlite, pine leaf soil, polyurethane, peanut shells, rice husks, vinasse, leaves, barks, EM (effective microorganisms), fruit peels, bran, bamboo leaves, calcium bicarbonate, complex enzymes, cortex albiziae, Lychnis coronata, peppers, Cortex Meliae, Changium smyrnioides, ferric sulfate, manganese sulfate, magnesium sulfate, calcium sulfate, zinc sulfate, sodium selenite, monopotassium phosphate, urea, potassium chloride, borax, a leavening agent and zeatin. The Pinus thunbergii Parl rejuvenation substrate has advantages that seedling growth can be promoted, seedling survival rate is increased, disease resistance and drought resistance of seedlings are improved, and high utilization rate is realized; a preparation method of the Pinus thunbergii Parl rejuvenation substrate is simple, easy in operation, low in investment and suitable for large-scale production and popularization.