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Industrial production method for wild thelephora ganbajun

A technology of dry bacteria and strains, applied in the field of industrial production of wild dry bacteria, to achieve the effect of high survival rate and short harvest period

Active Publication Date: 2015-12-23
昆明金摩尔食用菌种植有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Factory production has not been realized yet, so it is an important way to meet the needs of people and the market to develop a method that can realize large-scale and industrialized production of wild dried fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A. Pick fresh and strong wild Dried Bacteria, clean the weeds and soil, clean the Dried Dried Bacteria surface with 75% alcohol by volume, and then tear it longitudinally into pieces with a length of 3-5cm and a thickness of 1-2cm under aseptic conditions shape;

[0028] B. Inoculate the dried bacterium of the block obtained in step A in the following medium: 1000 parts of cornmeal, 1000 parts of soybean powder, 500 parts of pine oil, 10.5 parts of vitamin B, 1 part of magnesium sulfate, and a mass concentration of 50% glucose 550 parts of solution, 200 parts of calcium gluconate solution with a mass concentration of 10%, 0.5 parts of potassium dihydrogen phosphate, and 50 parts of yeast were cultured on a slant in a test tube at 23°C for 22 days to obtain D. For: CGMCCNO.990 2 ;

[0029] C. The preservation number is: CGMCCNO.9902 dry bacteria strains, inoculated into the culture medium of the nutrition bag, cut the pine tree roots that survived, 30cm in length and 1...

Embodiment 2

[0034] A. Pick fresh and strong wild Dried Bacteria, clean the weeds and soil, clean the Dried Bacteria surface with 75% alcohol by volume, and then tear it longitudinally into blocks with a length of 3cm and a thickness of 1cm under aseptic conditions;

[0035] B, inoculate the dried bacterium of the block shape obtained in step A in the following medium: 800 parts of cornmeal, 800 parts of soybean flour, 600 parts of pine oil, 10.2 parts of vitamin B, 1.5 parts of magnesium sulfate, and a mass concentration of 50% glucose 450 parts of solution, 300 parts of calcium gluconate solution with a mass concentration of 10%, 0.2 parts of potassium dihydrogen phosphate, and 40 parts of yeast were cultured on a slant in a test tube at 22°C for 25 days to obtain the strain of D. For: CGMCCNO.990 2 ;

[0036] C, the preservation number is: CGMCCNO.9902 dry bacillus bacterial classification, inoculate in the culture medium of nutrition bag, the pine tree root that will survive, length 2...

Embodiment 3

[0041] A. Pick fresh and strong wild Dried Bacteria, clean up the weeds and soil, clean the Dried Bacteria surface with 75% alcohol by volume, and then tear it longitudinally into blocks with a length of 5cm and a thickness of 2cm under aseptic conditions;

[0042] B, inoculate the dried bacterium of step A gained block in the following culture medium: 1200 parts of cornmeal, 1200 parts of soya bean powder, 300 parts of pine oil, 10.8 parts of vitamin B, 0.5 part of magnesium sulfate, the glucose that mass concentration is 50% 650 parts of solution, 180 parts of calcium gluconate solution with a mass concentration of 10%, 0.8 parts of potassium dihydrogen phosphate, and 60 parts of yeast were cultured on a slant in a test tube at 25°C for 20 days to obtain D. For: CGMCCNO.990 2 ;

[0043] C, the preservation number is: CGMCCNO.9902 dry bacillus bacterial classification, inoculate in the culture medium of nutrition bag, the pine tree root that will survive, length 35cm, diamet...

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Abstract

The invention discloses an industrial production method for wild thelephora ganbajun. A thelephora ganbajun strain is preserved in CGMCC, wherein the preservation number is CGMCC NO. 990(2). The production method comprises: longitudinally tearing disinfected wild thelephora ganbajun into blocks, and inoculating the wild thelephora ganbajun in a culture medium for slant culture to obtain a thelephora ganbajun strain; inoculating the thelephora ganbajun strain to a culture medium of a nutrition bag; inserting a disinfected pine root into the nutrition bag to be cultured; then dividing the second generation of thelephora ganbajun strain into number of parts and separately inoculating the pipe roots in the same culture medium to be cultured by a same method; and then culturing the whole bag in soil of the pine root, and carrying out cultivation under a certain condition till sporocarp is exposed out of the soil in 20-30 days to pick up the thelephora ganbajun. Therefore, large-scale and industrialized production of the wild thelephora ganbajun is realized. According to the thelephora ganbajun sporocarp, the original fragrance and nutrition of thelephora ganbajun are ensured. The thelephora ganbajun produced by the method disclosed by the invention is high in survival rate which can reach 99%.

Description

technical field [0001] The invention belongs to the technical field of wild edible fungus cultivation, and relates to an industrial production method of wild dried mushrooms. Background technique [0002] Thelephoraganbajun belongs to Basidiomyeo-ta, Hymenomyeetes, Aphyhoporales, Thelephoraceae, Thelephora, and is known as Thelephora in the market It also includes T.aurantiotineta, T.vialis, T.palmata, etc. Its fruiting body is branched from coral-like multiple times, and the base is thicker and fibrous. The leather is soft when it is young and then dry and tough when it is old. It has a beautiful smell and fragrance and is loved by consumers. It is widely distributed in Yunnan Province, including Kunming, Chuxiong, Yuxi, Lijiang, Baoshan, Qujing, Simao and other regions. [0003] Dried bacterium mainly grows in pine forests at an altitude of 1000-2200m, mainly in the coniferous forests of Yunnan dwarf pine, but also in pine-oak mixed forests or pure oak forests. ) has an ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 唐远芝
Owner 昆明金摩尔食用菌种植有限责任公司
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