The invention discloses a preparation method of spicy, crisp, safe and healthy pickled bamboo shoots. The preparation method comprises the following steps: removing impurities from fresh bamboo shoots, cleaning and slicing the fresh bamboo shoots, air-drying surface moisture, sending the bamboo shoots into a freeze-drying chamber, carrying out vacuum freeze-drying treatment, adding edible salt water and a plant extracting solution, carrying out uniform mixing, then sending the mixture into a high-pressure kettle for high-pressure permeation treatment, after microwave sterilization, adding a fermentation inoculant, performing uniform stirring and sealing, putting the mixture into the earth crust for primary fermentation, carrying out microwave sterilization, then adding lactobacillus plantarum, performing uniform stirring and sealing, putting the mixture into the earth crust for secondary fermentation, taking out the products, and carrying out microwave sterilization again to prepare the pickled bamboo shoots. The pickled bamboo shoots are spicy and crisp, shorten the fermentation time of the pickled bamboo shoots, are longer in preservation time, are beneficial to batch production, improve the processing technical level of the pickled bamboo shoots and the flavor of the products, can better improve the fermentation efficiency while ensuring the fragrance and taste of the bamboo shoots, can reduce the content of amino acid nitrogen and nitrite in the finished products, and have little harm to the human body.