31 results about "Ganoderma tsugae" patented technology

The invention relates to fungus plant cultivation, in particular to a ganoderma tsugae imitating wild short-cut wood cultivation method. The method comprises the steps of (1) strain preparation including (a) mother strain preparation, (b) protospecies preparation and (c) cultivar preparation, (2) short-cut wood cultivation including (a) mature material cultivation and (b) raw material cultivation and (3) harvesting which shall be conducted in time when the inner sides of pilei are golden yellow by means of observation. Ganoderma tsugae cultivated artificially is low in cost, high in yield, good in quality and high in effective constituent content, thereby having huge economic benefits, social benefits and ecological benefits.

The invention discloses a method for planting ganoderma tsugae by imitating wild growth in a forest land and belongs to the technical field of edible fungus cultivation. The method is finished by the steps of preparing ganoderma tsugae strains, preparing fungus sections in a production season, earthing in the forest land, managing grown ganoderma tsugae, managing during an underground fungus growing period, an overwintering period and a ganoderma tsuga growing period, controlling and preventing pests and diseases, and the like. The prepared fungus strains comprise a mother strain culture medium, a stock strain culture medium and a planting strain culture medium, wherein the mother strain culture medium comprises 200g of potato, 20g of glucose, 18 to 20g of agar and 1,000ml of water; the stock strain culture medium and the planting strain culture medium comprise 40 percent of broadleaf hard miscellaneous wood chips, 38 percent of pinevood chips, 20 percent of bran, 1 percent of white sugar and 1 percent of gypsum powder. According to the method provided by the invention, the quality and the medical components of lucid ganoderma can be effectively guaranteed, and the method is suitable for wide application.

The invention relates to an artificial cultivation method of lucid ganoderma, in particular to an artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr. The artificial cultivation method comprises the following steps: (1), a stock culture is prepared; (2), a pre-culture spawn is prepared; (3), a production spawn is prepared; (4), inoculating and cultivation are conducted; and (5), ganoderma tsugae murr growing management is conducted till a white edge of a fungi pileus is turned to yellow completely, and then the ganoderma tsugae murr is picked. According to the artificial cultivation method, the total biotransformation rate can reach 55-85%; the taste of the obtained ganoderma tsugae murr is relatively bitter; the ganoderma tsugae murr has the taste of ganoderma lucidum, and has effects of improving the immunity, preventing a cancer and the like; and requirements of the ganoderma tsugae murr in a wide range can be met through the artificial cultivation.

The invention discloses a fast-growing cultivation method for ganoderma tsugae, and relates to a fast-growing cultivation method. The method comprises the following steps of strain selecting, degrading bacterium preparing, culture medium preparing, raw material pretreating, material mixing and bagging, sterilizing, strain inoculating, hypha culturing, after-ripening managing, cultivation season selecting, land selecting and greenhouse building, special nutritional soil preparing, ganoderma tsugae fruiting mode determining, ganoderma tsugae fruiting managing, fruiting body harvesting, postharvest managing and the like. According to the novel ganoderma tsugae cultivation method, by reasonably scheduling the cultivation season, adopting low-cost pine tree wood chips as raw materials and integrating technologies such as raw material fermenting, variable-temperature-stimulative after-ripening managing and special nutritional soil preparing, the production cycle of the ganoderma tsugae is shortened to 1 year, the ganoderma tsugae fruiting yield in one year is equivalent to that in two years, the managing and producing cost is reduced, and very good economic benefits are achieved.

The invention provides a ganoderma tsugae strain cultivation method and particularly relates to the technical field of cultivation and processing of fungi. The method includes the four steps of mother strain cultivation, stock culture production, inoculation cultivation and fruiting management. The cultivated ganoderma tsugae strain is high in content of active ingredient and good in anti-tumor function and has good economic and social benefits, and the cultivation method is economical, practical, low in cost and good in effect; due to substrates mainly composed of grain materials and addition of humic acid, poison to the strain due to heavy metal in soil is reduced; the technique of simulating the growth environment of the wild ganoderma tsugae is adopted, so that yield of the ganoderma tsugae is increased while quality of the same is improved, and safety of the ganoderma tsugae products is guaranteed. The method is beneficial to standardization, industrialization and internationalization of the traditional Chinese medicine ganoderma tsugae products; quality of the ganoderma tsugae is improved highly, the active ingredient content is increased significantly, and good economic and social benefits are gained.

The invention discloses a method for cultivating edible fungi by means of wood blocks. A matrix of a traditional strain culture medium is changed, the matrix of strains and the matrix of the wood blocks are the same and can be fused into a whole, and harmonious coexistence can be achieved; the strains and a strain cover are combined into a whole and all communicated with xylon of the wood blocks, and the disengagement problem of a traditional strain cover is solved thoroughly. According to the technical scheme, the method for cultivating the edible fungi by means of the wood blocks comprises the following steps of wood block preparation, wherein timber is cut into long wood blocks for use, according to differences of cultivated varieties, the tree varieties of pine and Chinese fir are selected for ganoderma tsugae, and broadleaf wood is selected for other varieties; wood block strain making; wood block punching, wherein punching is conducted through a mechanical punching machine or an electric drill or a handmade hammer, and the hole depth, hole diameter, line spacing and spacing are determined according to the cultivated varieties and tree varieties; strain inoculation, wherein wood block strains are embedded in wood block holes, and tamping is conducted with a hammer till no gap exists between the hole walls and the strain blocks; spawn running, wherein a spawn running way is determined according to the technological requirements of different varieties of cultivated edible and medical fungi, differences of the tree species and the specific circumstances of sites; fruiting management.

The invention relates to a new strain of ganoderma tsugae Murr. and a cultivation method of the strain. The new strain of the ganoderma tsugae Murr. is ganoderma tsugae Murr. G122, and the preservation serial number is CGMCC 7220. The cultivation method of the ganoderma tsugae CGMCC 7220 comprises steps as follows: preparing a mother strain after tissue isolation of the strain; preparing a stock strain; preparing a production strain; performing cultivation; and managing ganoderma budding. The invention has the benefits as follows: the new strain of the ganoderma tsugae is provided and is from the root of Cyclobalanopsis glauca in a broad-leaved forest of Trashigang village, Bome county, Nyingchi prefecture, Tibet province, and the corresponding artificial cultivation method is further provided; and the new strain has a high active ingredient and a good antineoplastic function, enriches antineoplastic strains and has good economic benefits and social benefits.

The invention relates to a quality analysis analysis method of medicines or health care products, and specifically relates to a quality controlling method of a ganoderma aqueous extract. For controlling the product quality with high specificity, according to the invention, crude polysaccharide contents and a sum of ganoderma acid A, B, and C2 contents are adopted as quality controlling indexes of the ganoderma aqueous extract. According to the invention, ganoderma crude polysaccharide is extracted with a water extraction and alcohol precipitation method; polysaccharide with a glucan structure is precipitated by using a copper reagent; and the content of the polysaccharide is determined by using an ultraviolet spectrophotometry method. Therefore, the influences of auxiliary materials on determination are eliminated, and a basis is provided for quality controlling of the ganoderma aqueous extract. The contents of the characteristic components ganoderma acid A, B, and C2 in ganoderma lucidum, ganoderma sinnese, and ganoderma tsugae are relatively high. Compared to a currently commonly-used colorimetric method for determining total triterpenoid contents, the specificity of the method provided by the invention is high. With the method, purposes of authentication and quality control can be achieved.

The invention discloses a method for controlling the quality of a ganoderma lucidum alcohol extract. The method comprises the following steps of: taking the sum of ganoderic acid content A, B and C2 in the ganoderma lucidum alcohol extract as a mass control index; performing ultrasonic extraction on the ganoderma lucidum alcohol extract by taking methyl alcohol as a solvent; separating the ganoderic acid A from the ganoderic acid B through a high performance liquid chromatography column C8; separating the ganoderic acid C2 through a high performance liquid chromatography column C18; detecting by diode array detectors; and determining the nature according to the retention time of achromatographic peak, quantifying by a peak area external standard method, respectively measuring the ganoderic acid A content, the ganoderic acid B content and the ganoderic acid C2 content in a test sample, and calculating the total content, wherein ganoderma lucidum karst, ganoderma sinensis and ganoderma tsugae contain the characteristic components ganoderic acid A, ganoderic acid B and ganoderic acid C2 with relatively high content. Compared with the conventional method for measuring the total triterpene content by the colorimetric method, the method is high in specificity; and aims of authenticating ganoderma lucidum and controlling the quality can be achieved.

The invention discloses a ganoderma tsugae culture material for cultivation in Northeast. The culture material comprises a first-grade ganoderma tsugae strain culture material and a second-grade ganoderma tsugae strain culture material, wherein the first-grade ganoderma tsugae strain culture material is prepared from the following components in parts by weight: 150-250 parts of potato, 10-25 parts of glucose, 14-25 parts of agar powder, 2-4 parts of monopotassium phosphate, 1-2 parts of magnesium sulfate, 0.5-1.5 parts of peptone and 1000 parts of water; the second-grade ganoderma tsugae strain culture material is added with broad leaf wood bits and pine wood bits, so that an obvious promotion effect on the growth of second-grade hypha of the ganoderma tsugae is achieved, the growth coarse of the ganoderma tsugae is quickened, the growth period of the ganoderma tsugae is shortened, the yield of the ganoderma tsugae is effectively increased, and the property of the ganoderma tsugae for resisting external adverse environment is improved, so that the nutritive value of the grown ganoderma tsugae is remarkably increased.

The invention relates to a method for cultivating ganoderma tsugae by taking off a half of a bag. The method for cultivating ganoderma tsugae by taking off a half of the bag comprises the following steps: manufacturing ganoderma tsugae cultivating bacterium bags; after hyphae are matured, moving the bacterium bags to a ganoderma producing shelf; shearing a half of a plastic bag along the bag opening, and marking a 'V' shaped opening at the bottom part of the platic bg and performing a wall-type stacking; the wall-type stacking method is: stacking two bacterium bag openings inwards to form a wall, wherein the bacterium stack is 5 layers high, and the middle of two bacterium bags is reserved with a 10 cm distance, and soil fills in the middle, and 5 cm distance is kept between bags; stacking 5 cm soil and covering 5 cm soil again at the uppermost side; keeping the soil covering layer wet and performing regular fruiting period management; after the fruiting body is matured, harvesting. The method for cultivating ganoderma tsugae by taking off a half of the bag shears off a half of each of the plastic cultivating bacterium bags; outside oxygen is easy to enter the bag, and thus the oxygen in the bag is sufficient, and hypha growth is stronger; the ganoderma tsugae belongs to pollution-free edible mushrooms; after performing the wall-type stacking and soil coating, it is good for recovering the hyphae; the soil moistening effect is good; the ganoderma tsugae is not easy to get plant diseases and insect pests, and the output is high; moreover, the mushroom shape and size are easy to control, and quality is good; every bag can produce about 10 g dry product of ganoderma tsugae more averagely, thus the economical benefit is obviously improved.

The present invention discloses a kind of crude Ganoderma tsugae proteoglycan product and three kinds of purified Ganoderma tsugae proteoglycan products and their preparation process and application in preparing antitumor resisting medicine. The crude Ganoderma tsugae proteoglycan product is mixture including three kinds of proteoglycan, P1, P2 and P3 with the weight ratio of P1 1.50-3.00 wt%, P2 52-58 wt% and P3 20-25 wt%. Their polysaccharide part is uniform glucosan with glucosidic bond of beta-(1-3) configuration. Of the proteoglycans, P1 has n number of 500-600 and molecular weight 180-220 KDa; P2 has n number of 42-69 and molecular weight 15-25 KDa; and P3 has n number of 17-34 and molecular weight 6-12 KDa. Their protein part is combined to the reduction end of the polysaccharide part in integral form.

The invention provides a method for obtaining rare ginsenoside by the fermentation of Ganoderma tsugae liquid. The method comprises the following steps: manufacturing a Ganoderma tsugae mother strain into a liquid strain, inoculating ginseng or American ginseng into a water extract fermentation matrix for fermentation to obtain mycoplasma which contains the rare ginsenoside, wherein the volume ratio of the liquid strain to the liquid fermentation matrix is 1:(18-22). The invention provides the method for obtaining the rare ginsenoside by the fermentation of the Ganoderma tsugae liquid. Medicinal fungi and traditional Chinese medicines are combined through fermentation to obtain the fermentation mycoplasma. The method has the advantages of simple fermentation technology, easiness in operation and the like, is not harsh to environment requirements, and is favorable for industrial application and industry development. The product mycoplasma contains the rare ginsenoside Rg3, Rk1, Rg5 and C-K, meanwhile, a new thought and a new method are provided for the development and the utilization of the Ganoderma tsugae.

The invention provides a method for intercropping cultivation of acanthopanax sessiliflorus and ganoderma tsugae. The method comprises the following steps: cultivating acanthopanax sessiliflorus seedlings to form a plurality of planting rows; and when the height of the acanthopanax sessiliflorus reaches 0.8m-1.0m, cultivating ganoderma tsugae hyphal fragments among the plurality of planting rows. The method adopts the process for intercropping cultivation of the acanthopanax sessiliflorus and the ganoderma tsugae, and the utilization rate of lands is improved, so that previous lands which are only used for cultivating the acanthopanax sessiliflorus are used for cultivating the acanthopanax sessiliflorus and the ganoderma tsugae at the same time, and the utilization rate of the lands is improved by one time. With the adoption of the method provided by the invention, the space utilization efficiency also can be effectively improved; and the acanthopanax sessiliflorus mainly grows in middle and upper spaces, and the ganoderma tsugae grows in a lower space, so that the acanthopanax sessiliflorus and the ganoderma tsugae are not influenced by each other in space, the cultivation space is utilized very well, and the same space has more output. The ganoderma tsugae cultivated by the method has a proper growth speed, a moderate size and good quality, and the content of effective nutritional ingredients is high, so that the method is a cultivation mode capable of protecting forest resources and improving the quality of ganoderma.

The invention discloses a ganoderma tsugae culture material for cultivation in Northeast. The culture material comprises a first-grade ganoderma tsugae strain culture material and a second-grade ganoderma tsugae strain culture material, wherein the first-grade ganoderma tsugae strain culture material is prepared from the following components in parts by weight: 150-250 parts of potato, 10-25 parts of glucose, 14-25 parts of agar powder, 2-4 parts of monopotassium phosphate, 1-2 parts of magnesium sulfate, 0.5-1.5 parts of peptone and 1000 parts of water; the second-grade ganoderma tsugae strain culture material is added with broad leaf wood bits and pine wood bits, so that an obvious promotion effect on the growth of second-grade hypha of the ganoderma tsugae is achieved, the growth coarse of the ganoderma tsugae is quickened, the growth period of the ganoderma tsugae is shortened, the yield of the ganoderma tsugae is effectively increased, and the property of the ganoderma tsugae for resisting external adverse environment is improved, so that the nutritive value of the grown ganoderma tsugae is remarkably increased.

The invention relates to a quality analysis analysis method of medicines or health care products, and specifically relates to a quality controlling method of a ganoderma aqueous extract. For controlling the product quality with high specificity, according to the invention, crude polysaccharide contents and a sum of ganoderma acid A, B, and C2 contents are adopted as quality controlling indexes of the ganoderma aqueous extract. According to the invention, ganoderma crude polysaccharide is extracted with a water extraction and alcohol precipitation method; polysaccharide with a glucan structure is precipitated by using a copper reagent; and the content of the polysaccharide is determined by using an ultraviolet spectrophotometry method. Therefore, the influences of auxiliary materials on determination are eliminated, and a basis is provided for quality controlling of the ganoderma aqueous extract. The contents of the characteristic components ganoderma acid A, B, and C2 in ganoderma lucidum, ganoderma sinnese, and ganoderma tsugae are relatively high. Compared to a currently commonly-used colorimetric method for determining total triterpenoid contents, the specificity of the method provided by the invention is high. With the method, purposes of authentication and quality control can be achieved.

The invention relates to a mark of a Ganoderma tsugae 134 strain or a fruiting body molecule thereof, and an acquisition method and application thereof. The mark is a 422bp special DNA segment after PCR is amplified, and the sequences of a PCR amplimer pair are 5'-GTTCAGTTTCCGTGCCA-3' and 5'-CCGATGCTCCCTCTTG-3'. The acquisition method comprises the following steps: 1) extracting genome DNA of the strain or a fruiting body; 2) obtaining the special DNA segment; 3) obtaining a special PCR amplimer; 4) amplifying the genome DNA of the Ganoderma tsugae 134 strain or the fruiting body by the special PCR amplimer; and 5) performing agarose gel electrophoresis detection. The mark is used for rapidly identifying or detecting the genuineness of the Ganoderma tsugae 134 strain and Ganoderma lucidun products in the market. Compared with the conventional morphological detection, antagonism experiment and primodium test, the method has the advantages of short detection time and high accuracy.

The present invention provides a method of preventing or treating fibrosis in a subject, comprising administering an effective amount of an immunomodulatory protein derived from Ganoderma lucidum, Ganoderma lucidum, Ganoderma tsugae, Ganoderma microsporum or Ganoderma sinensis to the subject, thereby preventing or treating a fibrosis.

The present invention provides a method of preventing or treating fibrosis in a subject, comprising administering an effective amount of an immunomodulatory protein derived from Ganoderma lucidum, Ganoderma lucidum, Ganoderma tsugae, Ganoderma microsporum or Ganoderma sinensis to the subject, thereby preventing or treating a fibrosis.

The present invention discloses Ganoderma tsugae proteoglycan mixture and its preparation process and application. The Ganoderma tsugae proteoglycan mixture with molecular weight of 6-12 KDa is prepared through the following steps: 1. degreasing crushed Ganoderma tsugae sporophore with alcohol; 2. extracting with deionized water and collecting extracted liquid; 3. precipitating extracted liquid with alcohol; 4. dissolving the precipitate with water and microfiltering; and 5. ultrafiltering 50-80 KDa ultrafiltered filtrate with 3-5 KDa membrane and DEAE-Cellulose chromatographic separating the membrane intercepted matter to obtain the refined Ganoderma tsugae proteoglycan mixture. The Ganoderma tsugae proteoglycan mixture of the present invention has obvious effects of inhibiting tumor and raising immunity and thus high medicinal value.

The present invention discloses Ganoderma tsugae proteoglycan and its preparation process and application. The Ganoderma tsugae proteoglycan is prepared with Ganoderma tsugae sporophore, and has average molecular weight of 5-16 KDa. The Ganoderma tsugae proteoglycan consists of protein and polysaccharide in the weight ratio of 15.5-19.5 to 80-86. The polysaccharide consists of galactose, glucose, mannose and fructose in the proportion of 1.60-1.75 to 1.00 to 0.15-0.22 to 0.10-0.20, and has beta(1-->3) connected main chain. The Ganoderma tsugae proteoglycan of the present invention has obvious effects of inhibiting tumor and raising immunity and thus high medicinal value.

A Ganoderma tsugae active substance, produced from Ganoderma tsugae by following steps: (1) liquid culturing Ganoderma tsugae; (2) extracting the fermentation filtrate obtained with ethanol to obtain ethanol extraction precipitate; (3) purifying the ethanol extraction precipitate obtained by gel filtration chromatography to obtain gel filtration chromatographed product; and (4) extracting the gel filtration chromatographed product obtained with acetone to obtain the supernatant as the desired Ganoderma tsugae active substance. The invention provides further a composition comprising Ganoderma tsugae active substance produced by the above-described steps, said composition is useful for inducing or increasing the quantity of intranuclear transcription factor Nrf2, promoting the activities of antioxidant response element promoter (ARE promoter) and Thioredoxin reductases promoter (TrxR promoter), and inducing expressions of Heme oxygenase-1 (HO-1) and Thioredoxin reductases (TrxR), so as to achieve effects of protecting cell or preventing atherosclerosis.

The invention discloses a near-natural cultivation method and device for ganoderma tsugae. The cultivation method comprises the steps of strain manufacturing, cultivation environment and cultivation time selecting, cultivation mode selecting, harvesting and airing. The cultivation device comprises a cultivation bag processing and transferring structure and a rotary soil collecting structure; the cultivation bag processing and transferring structure at least comprises a clamping part and a sawtooth cutting piece, wherein the clamping part is arranged in a turnover mode and used for clamping a cultivation bag, and the sawtooth cutting piece faces the interior of the clamping part and is annularly arranged; and the rotary soil collecting structure comprises a rotary collecting part and a collecting cavity, the rotary collecting part is arranged outside the clamping part and used for digging a soil pit and collecting soil in the soil pit, the collecting cavity communicates with the rotarycollecting part and used for collecting soil, and the rotary collecting part is connected with the collecting cavity through a forward and reverse adjusting piece, and thus the collected soil can movein two directions in the rotary collecting part and the collecting cavity. By means of the mode, the cultivation efficiency and the cultivation quality are effectively improved.

The invention relates to a ganoderma tsugae active substance, produced by carrying out the following steps on ganoderma tsugae: (1) ganoderma tsugae is subject to liquid fermentation culture; (2) the fermentation filtration is extracted by ethyl alcohol, so as to obtain ethyl alcohol extraction sediment; (3) the obtained ethyl alcohol extraction sediment is subject to purification by colloid filtering chromatography, so as to obtain colloid filtering chromatographic product; (4) the obtained colloid filtering chromatographic product is extracted by acetone, and the obtained supernatant is the ganoderma tsugae active substance namely. The invention also provides ganoderma tsugae composition produced by the steps, the composition can be used for inducing or increasing the quantity of transcription factor Nrf2 in nucleus, improving the activity of antioxidation reaction segment promoter, improving the activity of sulphuric oxidoreduction protein enzyme promoter and inducing first type heme oxygenase and first type sulphuric oxidoreduction protein enzyme to express, so as to achieve the effect of cytoprotection or preventing arteriosclerosis.

The invention provides a method for intercropping cultivation of acanthopanax sessiliflorus and ganoderma tsugae. The method comprises the following steps: cultivating acanthopanax sessiliflorus seedlings to form a plurality of planting rows; and when the height of the acanthopanax sessiliflorus reaches 0.8m-1.0m, cultivating ganoderma tsugae hyphal fragments among the plurality of planting rows. The method adopts the process for intercropping cultivation of the acanthopanax sessiliflorus and the ganoderma tsugae, and the utilization rate of lands is improved, so that previous lands which are only used for cultivating the acanthopanax sessiliflorus are used for cultivating the acanthopanax sessiliflorus and the ganoderma tsugae at the same time, and the utilization rate of the lands is improved by one time. With the adoption of the method provided by the invention, the space utilization efficiency also can be effectively improved; and the acanthopanax sessiliflorus mainly grows in middle and upper spaces, and the ganoderma tsugae grows in a lower space, so that the acanthopanax sessiliflorus and the ganoderma tsugae are not influenced by each other in space, the cultivation space is utilized very well, and the same space has more output. The ganoderma tsugae cultivated by the method has a proper growth speed, a moderate size and good quality, and the content of effective nutritional ingredients is high, so that the method is a cultivation mode capable of protecting forest resources and improving the quality of ganoderma.

The invention discloses a method for controlling the quality of a ganoderma lucidum alcohol extract. The method comprises the following steps of: taking the sum of ganoderic acid content A, B and C2 in the ganoderma lucidum alcohol extract as a mass control index; performing ultrasonic extraction on the ganoderma lucidum alcohol extract by taking methyl alcohol as a solvent; separating the ganoderic acid A from the ganoderic acid B through a high performance liquid chromatography column C8; separating the ganoderic acid C2 through a high performance liquid chromatography column C18; detecting by diode array detectors; and determining the nature according to the retention time of achromatographic peak, quantifying by a peak area external standard method, respectively measuring the ganoderic acid A content, the ganoderic acid B content and the ganoderic acid C2 content in a test sample, and calculating the total content, wherein ganoderma lucidum karst, ganoderma sinensis and ganoderma tsugae contain the characteristic components ganoderic acid A, ganoderic acid B and ganoderic acid C2 with relatively high content. Compared with the conventional method for measuring the total triterpene content by the colorimetric method, the method is high in specificity; and aims of authenticating ganoderma lucidum and controlling the quality can be achieved.

The invention relates to a ganoderma tsugae active substance, produced by carrying out the following steps on ganoderma tsugae: (1) ganoderma tsugae is subject to liquid fermentation culture; (2) the fermentation filtration is extracted by ethyl alcohol, so as to obtain ethyl alcohol extraction sediment; (3) the obtained ethyl alcohol extraction sediment is subject to purification by colloid filtering chromatography, so as to obtain colloid filtering chromatographic product; (4) the obtained colloid filtering chromatographic product is extracted by acetone, and the obtained supernatant is theganoderma tsugae active substance namely. The invention also provides ganoderma tsugae composition produced by the steps, the composition can be used for inducing or increasing the quantity of transcription factor Nrf2 in nucleus, improving the activity of antioxidation reaction segment promoter, improving the activity of sulphuric oxidoreduction protein enzyme promoter and inducing first type heme oxygenase and first type sulphuric oxidoreduction protein enzyme to express, so as to achieve the effect of cytoprotection or preventing arteriosclerosis.

The invention relates to a new strain of ganoderma tsugae Murr. and a cultivation method of the strain. The new strain of the ganoderma tsugae Murr. is ganoderma tsugae Murr. G122, and the preservation serial number is CGMCC 7220. The cultivation method of the ganoderma tsugae CGMCC 7220 comprises steps as follows: preparing a mother strain after tissue isolation of the strain; preparing a stock strain; preparing a production strain; performing cultivation; and managing ganoderma budding. The invention has the benefits as follows: the new strain of the ganoderma tsugae is provided and is from the root of Cyclobalanopsis glauca in a broad-leaved forest of Trashigang village, Bome county, Nyingchi prefecture, Tibet province, and the corresponding artificial cultivation method is further provided; and the new strain has a high active ingredient and a good antineoplastic function, enriches antineoplastic strains and has good economic benefits and social benefits.

The present application discloses methods for extracting melanin from a fungus, where the fungus is Inonotus obliquus (chaga), Fomes fomentarius, Ganoderma tsugae, or Phellinus igniarius. In the methods, a sample of the fungus is dried and pulverized to form a powder sample. The powder sample is refluxed in a sodium hydroxide solution to form a mixture. The mixture is separated into a liquid portion and a solid portion via centrifugation or vacuum filtration, resulting in the liquid portion including the melanin extract. The separated liquid portion including the melanin is acidified with a hydrochloric acid solution to precipitate the melanin. The precipitated melanin is then isolated via centrifugation or vacuum filtration such that the melanin collects as the pellet or precipitate. The melanin precipitate is then purified by washing with dilute HCl and deionized, distilled water. The purified melanin is then dried to produce the extracted fungal melanin.

The present invention discloses a kind of crude Ganoderma tsugae proteoglycan product and three kinds of purified Ganoderma tsugae proteoglycan products and their preparation process and application in preparing antitumor resisting medicine. The crude Ganoderma tsugae proteoglycan product is mixture including three kinds of proteoglycan, P1, P2 and P3 with the weight ratio of P1 1.50-3.00 wt%, P2 52-58 wt% and P3 20-25 wt%. Their polysaccharide part is uniform glucosan with glucosidic bond of beta-(1-3) configuration. Of the proteoglycans, P1 has n number of 500-600 and molecular weight 180-220 KDa; P2 has n number of 42-69 and molecular weight 15-25 KDa; and P3 has n number of 17-34 and molecular weight 6-12 KDa. Their protein part is combined to the reduction end of the polysaccharide part in integral form.