Ganodema tsugae-proteoglycan and its preparing method and use

A technology of proteoglycan and Ganoderma lucidum, applied in pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of few researches on Ganoderma lucidum pine fir life extension effect

Active Publication Date: 2007-11-07
李彦群
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Less research has been done on Ganoderma tsugae Murill.

Method used

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  • Ganodema tsugae-proteoglycan and its preparing method and use
  • Ganodema tsugae-proteoglycan and its preparing method and use
  • Ganodema tsugae-proteoglycan and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Preparation of Pinus fir ganoderma proteoglycan crude product GTP

[0077] Weigh 1 kg of crushed pine, fir and Ganoderma lucidum fruiting bodies, add 8 L of 80% methanol, heat to 75°C, reflux for 3 hours, repeat 3 times, filter, combine the filtrates, recover methanol, add to the dregs equivalent to the weight of the fruiting body powder 15L of deionized water, heated and boiled for extraction, each time for 2 hours, repeated 6 times, filtered to obtain medicinal residues, 10L of 8% (containing 0.10% sodium borohydride) sodium carbonate solution was added to the medicinal residues, after fully stirring, 40 Soak for 30 hours at ℃, filter, adjust the pH of the filtrate to 6~7 with acetic acid, place it for clarification, centrifuge, collect the supernatant, add anhydrous methanol twice its volume, stir while adding, place for clarification, after centrifugation, dissolve the precipitate in In deionized water, after 1 μm microfiltration, then 5KD ultrafiltration,...

Embodiment 2

[0079] Example 2 Preparation of Pinus fir ganoderma proteoglycan crude product GTP

[0080] Weigh 1kg of crushed pine, fir and Ganoderma lucidum fruiting bodies, add 9L of 99% ethanol, heat at 80°C, reflux extraction, 4 hours each time, repeat 5 times, filter to obtain medicinal residues, add 10L of deionized water to the medicinal residues, and boil Extraction, 3 hours each time, repeated 4 times, filtered to obtain medicinal residues, 12L of 6% (containing 0.08% sodium tetraborohydride) sodium hydroxide solution was added to the medicinal residues, fully stirred, soaked at 30°C for 30 hours, filter, the filtrate was adjusted to pH 6-7 with hydrochloric acid, placed for clarification, centrifuged, collected the supernatant, added absolute ethanol twice its volume, stirred while adding, placed for clarification, centrifuged, dissolved the precipitate in deionized water, After 3 μm microfiltration, 3KD ultrafiltration, concentration, and freeze-drying, the present invention-Gan...

Embodiment 3

[0081] Embodiment 3 Change the concentration of ethanol into 80% or 95% in Example 2, reflux extraction for each 3 hours or 6 hours, repeat 6 times or 4 times, change the concentration of sodium hydroxide to 3% or 8%, and extract the temperature Change to 25°C or 40°C, and other conditions remain unchanged, and the crude product of GTP can also be obtained, and the yield is basically the same.

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PUM

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Abstract

The present invention discloses a kind of crude Ganoderma tsugae proteoglycan product and three kinds of purified Ganoderma tsugae proteoglycan products and their preparation process and application in preparing antitumor resisting medicine. The crude Ganoderma tsugae proteoglycan product is mixture including three kinds of proteoglycan, P1, P2 and P3 with the weight ratio of P1 1.50-3.00 wt%, P2 52-58 wt% and P3 20-25 wt%. Their polysaccharide part is uniform glucosan with glucosidic bond of beta-(1-3) configuration. Of the proteoglycans, P1 has n number of 500-600 and molecular weight 180-220 KDa; P2 has n number of 42-69 and molecular weight 15-25 KDa; and P3 has n number of 17-34 and molecular weight 6-12 KDa. Their protein part is combined to the reduction end of the polysaccharide part in integral form.

Description

technical field [0001] The invention relates to the proteoglycan extracted from the fruit body of Ganoderma tsugae Murrill. and a preparation method thereof, and also relates to the application of the proteoglycan in antitumor drugs. Background technique [0002] At present, the clinical treatment of tumor diseases mainly adopts surgery, radiotherapy, and chemotherapy. Because radiotherapy and chemotherapy seriously kill the normal cells and tissues of the human body, the physical fitness of many patients declines rapidly, and even continuous treatment is impossible. Therefore, people are constantly looking for new anticancer drugs with low toxicity and high tumor inhibition rate. Since the occurrence and spread of tumors are closely related to the immune function of the human body, tumor immunotherapy has been developed. In the middle and late twentieth century, polysaccharide drugs derived from fungi, including versicolor polysaccharide, lentinan, and schizophyllan, all ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A61K31/716A61P35/00
Inventor 费晓方
Owner 李彦群
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