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New strain of ganoderma tsugae Murr. and cultivation method of strain

A technology of pine fir ganoderma and a cultivation method, which are applied to the new strain of Pine fir ganoderma and its cultivation field, can solve problems such as no research reports on strains, and achieve good anti-tumor function, good economic and social benefits, and active ingredients. high effect

Active Publication Date: 2014-07-30
GUANGDONG INST OF MICROORGANISM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This application is a new bacterial species found and isolated from the roots of Pseudomonas chinensis in the broad-leaved forest of Zhaxigang Township, Bomi County, Nyingchi Prefecture, Tibet. After research, there is no research report on this bacterial species

Method used

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  • New strain of ganoderma tsugae Murr. and cultivation method of strain
  • New strain of ganoderma tsugae Murr. and cultivation method of strain
  • New strain of ganoderma tsugae Murr. and cultivation method of strain

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The artificial cultivation method of the new strain of ganoderma lucidum (CGMCC7220) comprises the following steps:

[0025] 1) According to the mass percentage, it is composed of 20% potato, 2% glucose, 2% agar, 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, trace vitamin B1, and the rest is water. Conventional production of comprehensive PDA slope, 0.11MPa atmospheric pressure , 121 ℃ high temperature and high pressure damp heat sterilization for 30 minutes;

[0026] 2) Calculated by mass percentage, it is 78% sorghum, 20% millet, and CaCO 3 2% raw material composition, water content 60%, make the original seed material, put the original seed material into a 13cm×25cm high temperature resistant transparent polypropylene strain bag, use a small wooden stick to make holes in the bag material, the hole is as deep as the bag At the bottom, put a plastic ring on the mouth of the bag, and buckle the matching lid to make the original seed bag, which is equiva...

Embodiment 2

[0034] Example 2: Analysis of the active components of the new strain of Ganoderma lucidum (CGMCC7220) (Ganoderma lucidum polysaccharides and total triterpenes)

[0035]In this example, three samples were used to determine the content of Ganoderma lucidum polysaccharides and total triterpenes. The three samples were: the fruiting body of Chizhi lucidum commonly used in China (commercially available), the fruiting body of Ganoderma lucidum CGMCC7220 (artificially cultivated in Guangzhou), and the fruiting body of Pinus fir Fruiting bodies of Ganoderma lucidum CGMCC7220 (cultivated in Tibet).

[0036] 1. Determination of Ganoderma lucidum polysaccharide content

[0037] 1) Preparation of glucose standard curve

[0038] Accurately weigh 50mg of standard glucose dried at 105°C with constant weight, put it in a 500ml volumetric flask, add distilled water to dissolve and dilute to the mark. Draw glucose standard solution 0.00ml, 0.20ml, 0.40ml, 0.60ml, 0.80ml, 1.00ml, divide into ...

Embodiment 3

[0062] Example 3: Analysis of the anti-tumor properties of the new strain of Ganoderma lucidum (CGMCC7220) (in vitro tumor breast cancer cell MT-1 growth inhibition experiment)

[0063] 1. Preparation of water extract

[0064] After the fruit bodies of Chizhi lucidum and Ganoderma lucidum CGMCC7220 were crushed, distilled water was added according to the ratio of solid to liquid at 1:10, kept in a water bath at 90°C for 3 hours, filtered, and the filter residue was repeatedly extracted for 2 hours, filtered, and the combined filtrate was concentrated until the material liquid was After 1:5, add 5 times the volume of absolute ethanol and let it stand for 1 hour, then filter, and freeze-dry the precipitate after alcohol precipitation (the freeze-drying instrument is CHRIST ALPHA1-4LD made in Germany) for later use.

[0065] 2. Tumor cell culture

[0066] Cells were cultured in DMEM medium containing penicillin, streptomycin and 10% fetal bovine serum (FBS). The DMEM cell suspe...

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Abstract

The invention relates to a new strain of ganoderma tsugae Murr. and a cultivation method of the strain. The new strain of the ganoderma tsugae Murr. is ganoderma tsugae Murr. G122, and the preservation serial number is CGMCC 7220. The cultivation method of the ganoderma tsugae CGMCC 7220 comprises steps as follows: preparing a mother strain after tissue isolation of the strain; preparing a stock strain; preparing a production strain; performing cultivation; and managing ganoderma budding. The invention has the benefits as follows: the new strain of the ganoderma tsugae is provided and is from the root of Cyclobalanopsis glauca in a broad-leaved forest of Trashigang village, Bome county, Nyingchi prefecture, Tibet province, and the corresponding artificial cultivation method is further provided; and the new strain has a high active ingredient and a good antineoplastic function, enriches antineoplastic strains and has good economic benefits and social benefits.

Description

technical field [0001] The invention relates to a new strain of pine fir ganoderma and a cultivation method thereof. Background technique [0002] Ganoderma tsugae Murr. is included in the Pharmacopoeia of Traditional Chinese Medicine in my country, and is widely used in anti-tumor drugs and health food together with Ganoderma lucidum and Ganoderma sinense Zhao, Xu et Zhang. At present, the patent applications on Ganoderma lucidum are mainly related to the extraction of Ganoderma lucidum, preparation of polysaccharides, molecular markers of fruiting bodies or cultivation methods of existing strains. This application is a new bacterial species found and isolated from the roots of Pleurotus sativa in the broad-leaved forest of Zhaxigang Township, Bomi County, Nyingchi Prefecture, Tibet. After research, there is no research report on this bacterial species. Contents of the invention [0003] Aiming at the above deficiencies, the present invention provides a new strain of Pin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 胡惠萍周振辉
Owner GUANGDONG INST OF MICROORGANISM
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