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Method for measuring ganoderic acid A, B, C2 content in ganoderma lucidum alcohol extract

A determination method and technology of ganoderma acid, applied in the field of analytical chemistry, can solve the problems of false positive and poor specificity, and achieve the effect of strong specificity

Inactive Publication Date: 2013-12-18
福建省食品药品质量检验研究院 +1
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Problems solved by technology

At present, the quality control of the ethanol extract of Ganoderma lucidum mostly uses ultraviolet spectrophotometry to determine the content of total triterpenes. This method often has false positives and poor specificity.

Method used

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  • Method for measuring ganoderic acid A, B, C2 content in ganoderma lucidum alcohol extract
  • Method for measuring ganoderic acid A, B, C2 content in ganoderma lucidum alcohol extract
  • Method for measuring ganoderic acid A, B, C2 content in ganoderma lucidum alcohol extract

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Embodiment Construction

[0021] 1. Raw material requirements

[0022] 1) Chizhi and Zizhi should meet the requirements of Part I of the Pharmacopoeia of the People's Republic of China (2010 Edition).

[0023] 2) Pine and fir Ganoderma lucidum should meet the following requirements:

[0024] Scope: raw material requirements are suitable for pine fir ganoderma (Ganoderma tsugae Murr. ） subentity.

[0025] Table 1 Sensory Requirements

[0026]

[0027] Table 2 physical and chemical indicators

[0028]

[0029] 2. Preparation method of ethanol extract of Ganoderma lucidum

[0030] Ganoderma lucidum is crushed into coarse particles, soaked in 8-10 times the amount of 90% ethanol for more than 2 hours, heated and kept at a slight boil (70°C-80°C) for 2 hours, filtered, and then added 8-10 times the amount of 90% ethanol, Heat and keep boiling slightly for 2 hours, filter, combine the two filtrates, concentrate and recover ethanol, measure the total solid content when concentrated to near drynes...

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Abstract

The invention discloses a method for controlling the quality of a ganoderma lucidum alcohol extract. The method comprises the following steps of: taking the sum of ganoderic acid content A, B and C2 in the ganoderma lucidum alcohol extract as a mass control index; performing ultrasonic extraction on the ganoderma lucidum alcohol extract by taking methyl alcohol as a solvent; separating the ganoderic acid A from the ganoderic acid B through a high performance liquid chromatography column C8; separating the ganoderic acid C2 through a high performance liquid chromatography column C18; detecting by diode array detectors; and determining the nature according to the retention time of achromatographic peak, quantifying by a peak area external standard method, respectively measuring the ganoderic acid A content, the ganoderic acid B content and the ganoderic acid C2 content in a test sample, and calculating the total content, wherein ganoderma lucidum karst, ganoderma sinensis and ganoderma tsugae contain the characteristic components ganoderic acid A, ganoderic acid B and ganoderic acid C2 with relatively high content. Compared with the conventional method for measuring the total triterpene content by the colorimetric method, the method is high in specificity; and aims of authenticating ganoderma lucidum and controlling the quality can be achieved.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a kind of ganoderma acid A, B, C in the ethanol extract of ganoderma lucidum 2 method for the determination of content. Background technique [0002] Ganoderma lucidum Ganoderma lucidum (Leyss. ex Fr.) Karst. or Zizhi Ganoderma sinense Zhao, Xu et Zhang or Ganoderma lucidum Ganoderma The dry fruiting body of tsugae Murr. has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. Studies have shown that Ganoderma lucidum mainly contains polysaccharides, triterpenes, nucleosides, alkaloids, flavonoids, amino acids and trace elements and other chemical components, which have the functions of anti-tumor, immune regulation, liver protection, anti-oxidation, blood pressure lowering, and blood fat reduction. . At present, pharmacological activity research mainly focuses on Ganoderma lucidum polysaccharides and Ganoderma triterpenoids, ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 王勇陈硕卢端萍蒋婷婷范明李晔吴长辉
Owner 福建省食品药品质量检验研究院
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