Mark of Ganoderma tsugae 134 strain or fruiting body molecule thereof, and acquisition method and application thereof

A technology of pine, fir, ganoderma and molecular markers, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of short detection time and high accuracy

Inactive Publication Date: 2009-08-26
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SCAR (Sequence Characterized Amplified Region) marker is a very stable molecular marker, which has the characteristics of rapid, sim

Method used

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  • Mark of Ganoderma tsugae 134 strain or fruiting body molecule thereof, and acquisition method and application thereof
  • Mark of Ganoderma tsugae 134 strain or fruiting body molecule thereof, and acquisition method and application thereof

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Embodiment 1

[0056] Strains: Collected 140 strains of Ganoderma lucidum production from China and 164 strains in total from 24 strains of ATCC. , Ganoderma lucidum, Ganoderma lucidum, and Ganoderma sessile more than 10 species. PCR amplification was performed on a total of 165 samples of their genome DNA and the genome (number 190) of the fruiting body of Ganoderma lucidum No. 134.

[0057] 1. Genomic DNA extraction

[0058] 1. Mycelia Culture and Genomic DNA Extraction

[0059] Transfer the Pine, Shanshan and Ganoderma lucidum 134 strains preserved at 4-6°C to a PDA slant, cultivate and activate at 26-28°C, and transfer to

[0060] In the potato glucose culture solution, shake culture at 26-28 for 10-14 days, collect mycelium, and extract genomic DNA by LETS method; DNA is diluted to 10-20ng / μL, and stored at -20°C.

[0061] 2. Extraction of Genomic DNA from Fruiting Bodies

[0062] ①Cut 1-2g of the fruiting body into pieces, and use 95% (V / V) alcohol and 0.5mol / L EDTANa with a volume...

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Abstract

The invention relates to a mark of a Ganoderma tsugae 134 strain or a fruiting body molecule thereof, and an acquisition method and application thereof. The mark is a 422bp special DNA segment after PCR is amplified, and the sequences of a PCR amplimer pair are 5'-GTTCAGTTTCCGTGCCA-3' and 5'-CCGATGCTCCCTCTTG-3'. The acquisition method comprises the following steps: 1) extracting genome DNA of the strain or a fruiting body; 2) obtaining the special DNA segment; 3) obtaining a special PCR amplimer; 4) amplifying the genome DNA of the Ganoderma tsugae 134 strain or the fruiting body by the special PCR amplimer; and 5) performing agarose gel electrophoresis detection. The mark is used for rapidly identifying or detecting the genuineness of the Ganoderma tsugae 134 strain and Ganoderma lucidun products in the market. Compared with the conventional morphological detection, antagonism experiment and primodium test, the method has the advantages of short detection time and high accuracy.

Description

technical field [0001] The invention belongs to the field of identification of ganoderma lucidum strains, and in particular relates to a pine fir ganoderma lucidum 134 strain or its fruiting body molecular marker, its obtaining method and application. Background technique [0002] According to "Shen Nong's Materia Medica" and "Compendium of Materia Medica", Ganoderma lucidum is beneficial to the heart, nourishes the heart and generates blood, helps the heart to fill the pulse, calms the nerves, nourishes the spleen and lungs, strengthens the muscles and bones, sharpens the joints, and cures deafness. Modern studies have found that Ganoderma lucidum not only has curative effects on cancer, cerebral hemorrhage, and heart disease, which are the three major causes of death in humans, but also has significant curative effects on diseases such as gastrointestinal, liver, kidney, leukemia, neurasthenia, chronic bronchitis, asthma, and allergies. Lin Zhibin believes that Ganoderma l...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 唐传红张劲松杨焱唐庆九贾薇刘艳芳周帅刘方冯娜
Owner SHANGHAI ACAD OF AGRI SCI
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