Ganoderrma tsugae-proteoglycan and its preparing method and use

A technology of proteoglycan, pine fir and Ganoderma lucidum, applied in the application of active ingredients, proteoglycan and its extraction field, to achieve the effect of high purity, high tumor inhibition rate and low toxicity

Active Publication Date: 2007-11-14
李彦群
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on Ganoderma tsugae Murrill. is rarely reported. Among them, only in 2003, Taiwan International Famous University declared the title "Polysaccharide-based extract from ganoderma, pharmaceutical use thereof, and process for preparing the same" in the United States. The patent

Method used

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  • Ganoderrma tsugae-proteoglycan and its preparing method and use
  • Ganoderrma tsugae-proteoglycan and its preparing method and use
  • Ganoderrma tsugae-proteoglycan and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, preparation pine fir Ganoderma lucidum protein-polysaccharide

[0021] Weigh 100 grams of pine fir ganoderma fruiting body powder, add 1 liter of 85% ethanol, heat and reflux at 75°C for 2 hours, repeat 3 times, filter, add 2 liters of deionized water to the medicinal residue, heat to boil, stir at intervals, each time Extract for 3 hours, repeat 5 times, filter, combine extracts, concentrate to a volume of 0.5-1 liter, cool to room temperature, add 2.5 liters of absolute ethanol, stir while adding, place at 4°C for clarification, centrifuge (7000-8000rpm, 15min), take the precipitate and dissolve it in water, microfilter at 10μm and 1μm, take the filtrate, then ultrafilter it through 80KD, take the filtrate, and then use a 3KD membrane for ultrafiltration, and the obtained retentate is dissolved in deionized water and balanced on the surface DEAE-Cellulose (Cl - ) column (3 × 75cm), the loading flow rate is 0.25ml / min, then deionized water is used as the...

Embodiment 2

[0022] Embodiment 2, preparation of Pinus fir Ganoderma lucidum protein-polysaccharide

[0023]Weigh 100 grams of pine fir ganoderma fruiting body powder, add 1.5 liters of 80% methanol, heat and reflux at 80°C for 3 hours, repeat 5 times, filter, add 4 liters of deionized water to the medicinal residue, heat to boil, stir at intervals, each time Extract for 3 hours, repeat 6 times, filter, combine the filtrates, concentrate to a volume of 1 liter, cool, add 4 liters of anhydrous methanol, stir while adding, place at 4°C for clarification, centrifuge (8000rpm, 15min), the precipitate is dissolved in water, After 10 μm, 5 μm and 1 μm microfiltration, take the filtrate and pass it through an ultrafiltration membrane of 80KD, take the filtrate, and then use a 3KD membrane for ultrafiltration, and the obtained retentate is dissolved in deionized water, and put on a well-balanced Sepharose-CL6B column (3× 70cm), the sample loading flow rate is 0.6ml / min, then use deionized water as...

Embodiment 3

[0024] Example 3, Preparation of Pinus fir Ganoderma lucidum protein-polysaccharide

[0025] Weigh 50kg of pine, fir, ganoderma fruiting body powder into an extraction tank, add 450 liters of 85% ethanol, under stirring, reflux extraction at 75-80°C for 2 hours each time, repeat 3 times, recover ethanol, add water to 1000 liters of dregs , heated to 100°C, extracted for 3 hours, repeated 5 times, filtered, combined the filtrates, concentrated to a volume of 250 liters, added 5 times the volume of absolute ethanol under stirring, placed at a low temperature of 4°C, clarified, centrifuged (8000rpm, 15min), the precipitate was dissolved in deionized water, passed through 10μm microfiltration, 1μm microfiltration, and then 80KD ultrafiltration, then concentrated with a 3KD membrane, and the obtained retentate was dissolved in deionized water, and the balanced DEAE-Sepharose (F.F.) column (3×80cm), the sample loading flow rate is 0.4ml / min, then use deionized water as the mobile ph...

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Abstract

The present invention discloses Ganoderma tsugae proteoglycan and its preparation process and application. The Ganoderma tsugae proteoglycan is prepared with Ganoderma tsugae sporophore, and has average molecular weight of 5-16 KDa. The Ganoderma tsugae proteoglycan consists of protein and polysaccharide in the weight ratio of 15.5-19.5 to 80-86. The polysaccharide consists of galactose, glucose, mannose and fructose in the proportion of 1.60-1.75 to 1.00 to 0.15-0.22 to 0.10-0.20, and has beta(1-->3) connected main chain. The Ganoderma tsugae proteoglycan of the present invention has obvious effects of inhibiting tumor and raising immunity and thus high medicinal value.

Description

technical field [0001] The invention relates to a proteoglycan and its preparation method and application, in particular to a proteoglycan obtained from Ganoderma tsugae Murrill., an extraction method thereof and the application of the proteoglycan as an active ingredient of a medicine. Background technique [0002] At present, there are three main ways to treat tumor diseases clinically: surgery, radiotherapy and chemotherapy. However, radiotherapy and chemotherapy seriously damage the normal cells and tissues of the human body, causing many patients to decline rapidly, and even cannot continue treatment. For a long time, people have been looking for new anticancer drugs with the characteristics of low toxicity and high tumor inhibition rate. Since the occurrence and spread of tumors are closely related to the immune function of the human body, tumor immunotherapy has been developed. In the middle and late twentieth century, some polysaccharide drugs derived from fungi wer...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 费晓方
Owner 李彦群
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