119results about How to "Improve tumor inhibition rate" patented technology

The invention discloses an irinotecan or irinotecan hydrochloride lipidosome and a preparation method thereof. The lipidosome contains irinotecan or irinotecan hydrochloride, neutral phospholipid and cholesterol, wherein the weight ratio of the cholesterol to the neutral phospholipid is 1:(3-5), and the irinotecan or irinotecan hydrochloride lipidosome is prepared by an ion gradient method.

The invention discloses a traditional Chinese medicine composition having an anticancer effect and a preparation method thereof. The traditional Chinese medicine composition is a medicament mixture composed of a polyphyllin extract and a turmeric water extract in a mass ratio of 2:3. The preparation process of the traditional Chinese medicine composition comprises the following steps: preparing the polyphyllin extract; preparing the turmeric water extract; and preparing a mixture of the polyphyllin extract and the turmeric water extract. The traditional Chinese medicine composition has the advantages that: the preparation process is simple; the obtained traditional Chinese medicine composition has the anticancer effect, and the anticancer activity of the composition is high and has broad spectrum; and compared with singly applied polyphyllin extract or turmeric water extract, the composition has stronger antitumour activity, smaller toxic and side effects and immunity-enhancing function.

The invention relates to multipiptide protein active substance in a kind of bloody clam (Arca subcrenata Lischke; Scapharca subcrenata), the prepn. method and the uses. The prepn. method is that bloody clam is homogenated to obtain superantant which is treated by chromatography in ion exchange column, desalt, chromatograph separation in gel colum to be combined to several peaks with molecular weight 3-15 KDa which is concentrated and dried to obtain multipeptide protein active substance. Said active substance has 73.72 percent of cancer-inhibiting ratio and can be made as anti-cancer medicine.

The invention discloses a gambogic acid cyclized analog, a preparation method and application thereof. The semi-synthesized gambogic acid cyclized analog is obtained by the extracted and purified gambogic acid through chemical synthesis and has a structural general formula I-III as the right formula, wherein substituent groups of A ring, B ring, C ring, R1, R2, R3, R4, R5, R6, R7, R7, R8, R9, R10, R11 or / and R12 contain glycosyl group, polyhydroxy, amino acid group, acyloxy group, phosphoric acid oxygen group, sulphonic acid oxygen group, alkoxyl group, aromatic oxygen group, heterocyclic oxygen group, mercapto group, substituted mercapto group, primary amine and secondary amines groups or / and substituted primary amine and secondary amines groups, and chain hydrocarbon or and cyclic group containing oxygen, sulphur, nitrogen, carbon or / and phosphor atoms, and one or combination of the substitution groups. The gambogic acid cyclized analog has anti-tumor, antivirus, antibacterial and antifungal pharmacology activities, can be used as anti-tumor, antivirus, immune, antibacterial and antifungal medicaments, and can be applied together with the known anti-tumor, antivirus, immune, antibacterial and antifungal medicaments.

The invention discloses a preparation method of bone mesenchymal stem cells carrying NK4 genes and application thereof in medicine for treating gastric cancer. The preparation method comprises the following steps: extracting the NK4 genes from an HGF plasmid, using an in-Fusion technology to directionally clone the NK4 genes to a slow virus expression carrier plasmid, constructing an NK4-EGFP fusion gene recombinant slow virus carrier plasmid (pGC-FU-NK4) and then co-transfecting a 293T cell with the pGC-FU-NK4, a pHelper1.0 carrier and a pHelper2.0 carrier, packaging to produce NK4 overexpression slow virus particles (Lenti-NK4), using slow virus (Lenti-NK4) carrying NK4-EGFP fusion gene to transfect the bone mesenchymal stem cells to establish in vitro bone mesenchymal stem cells to stably express the NK4 genes. Balb / C nude mice animal experiments prove that the NK4 gene therapy taking BMSCs as a carrier can obviously inhibit the growth of subcutaneous transplant tumor of gastric cancer with high tumor inhibition efficiency, and the NK4 gene therapy is a good gene therapeutic method for gastric cancer.

The invention relates to a TPGS modified docetaxel liposome nano-drug delivery system, a preparation method and an application thereof. The TPGS modified docetaxel liposome nano-drug delivery system uses phospholipid and cholesterol as membrane materials, Surfactant TPGS modified liposomes and docetaxel (DTX) as anticancer drug were prepared by membrane dispersion ethanol injection ultrasonic dispersion reverse evaporation and freeze drying. The particle size of nano-doxetaxel liposomes modified by TPGS is 80-200 nm, the zeta potential is 0+30 mV or 0-30 mV, the encapsulation efficiency is 75-99%, with a drug loading of 3-15%. The in vitro cell experiment and in vivo pharmacodynamic experiment of the TPGS modified docetaxel liposome nano-drug delivery system prepared by the invention showthat the nano-drug delivery system can inhibit P. Overexpression of gp, decrease of drug efflux, increase of drug enrichment in tumor cells, increase of cytotoxicity and apoptosis rate, increase of tumor therapeutic effect and reversal of multidrug resistance.

The invention discloses a tumor deoxyribonucleic acid (DNA) vaccine taking mucin 1 and survivin as targets and a viral vector vaccine. The invention relates to the field of tumor DNA vaccines and viral vector vaccines, in particular to recombinant DNA vector VR-S8 and VR-MS, recombinant adenovirus Ad-S8 and Ad-MS, and recombinant poxvirus MVA-MS vaccines, and application of the optimum combination of immune ways between DNA vaccines and viral vector vaccines and between the viral vector vaccines in preparing antitumor vaccines.

The invention relates to isoalantolactone derivatives and salts thereof and provides an isoalantolactone derivative as shown in a formula (I). Salification acids are selected from inorganic acids or organic acids; the inorganic acids are selected from hydrofluoric acid, hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, nitric acid, phosphoric acid, carbonic acid, boric acid, seleninic acid, phosphomolybdic acid, phosphorous acid and sulphurous acid; the organic acids are selected from citric acid, maleic acid, D-malic acid, L-malic acid, DL-malic acid, L-lactic acid, D-lactic acid, DL-acid, oxalic acid, methylsulfonic acid, pentanoic acid, oleic acid, lauric acid, p-toluene sulfonic acid, 1-naphthalenesulfonic acid, 2-naphthalenesulfonic acid, phthalic acid, tartaric acid, malonic acid, succinic acid, fumaric acid, glycolic acid, mercaptan acid, glycine, sarcosine, sulfonic acid, nicotinic acid, methylpyridine acid, isonicotinic acid, benzoic acid or substituted benzoic acid.

The invention discloses a method for refrigerating, vacuum-drying and purifying ganoderma spore powder, which comprises the following steps of: adding water into ganoderma spore powder raw material while stirring and uniformly dispersing the ganoderma spore powder raw material; standing, removing upper layer floating matter and removing deposit; filtering intermediate solution and removing impurities; filtering and pressing filtrate, smashing the filtrate, adding purified water, mixing the filtrate with the purified water uniformly to obtain mixed suspension and carrying out refrigeration vacuum drying process to obtain the ganoderma spore powder. The method enhances the cleanliness, purity and bioactivity, keeps physical properties such as color, fragrance and flavor and the like, and can ensure that ganoderma spore biological protective film which can cause immuno reaction on human body is fully removed and the ganoderma spore powder truly becomes a kind of purely natural safe greenfood; and the obtained ganoderma spore powder has a low moisture content and is beneficial to long-time storage of product. The ganoderma spore powder refrigerated and dried by adopting the method isloose in texture. The method is beneficial to reduction of wall damaging times and improvement on the wall damaging rate, and can remarkably improve the tumor inhibition rate of the ganoderma spore powder.

The invention discloses application of amino-acid-modified metallofullerene water-soluble nanoparticles in the preparation of tumor vascular disrupting agents; the amino-acid-modified metallofullerene water-soluble nanoparticles have a structural general formula: metallofullerene-(OH)x(amino acid)y, wherein metallofullerene is metallic fullerene, with 0< / =x< / =50 and 0< / =y< / =50, and amino acid is a water-soluble amino acid. The tumor vascular disrupting agents prepared by applying the amino-acid-modified metallofullerene water-soluble nanoparticles to their preparation have higher biosafety, have zero toxic and side effects for normal biological tissues, are low in dosage and are good in treatment effect.

The invention discloses a brain targeted medicine lipidosome preparation as well as a preparation method and an application thereof. The brain targeted medicine lipidosome which has an enhancement effect is prepared by taking oligopeptide with arginine residues as a target molecule and by using a thin film hydration method and a three-bottle method, and the technical problems that a conventional lipidosome preparation cannot penetrate through blood brain barrier and has no brain targeting are solved. The galenic pharmacy observation shows that the encapsulation efficiency and the particle size of the brain targeted medicine lipidosome preparation disclosed by the invention meet the medicine requirements, and compared with ordinary lipidosome, the brain targeted medicine lipidosome disclosed by the invention is good in acid sensitivity and good in stability in serum, and has in-vitro cell medicine effect and in-vivo mouse glioma medicine effect which are higher than those of long circulation Doxorubicin lipidosome.

The invention discloses polyethylene glycol vitamin E succinate and calprotectin modified nanoparticle and a preparation method thereof. The polyethylene glycol vitamin E succinate and calprotectin modified nanoparticle is prepared from the following raw materials in parts by weight: 1 part of active medicine, 20 to 40 parts of phospholipid, 2 to 4 parts of sophorolipid, 1 to 2 parts of calprotectin, and 5 to 10 parts of polyethylene glycol vitamin E succinate. Compared with the prior art, the nanoparticle modified by olyethylene glycol vitamin E succinate, sophorolipid and calprotectin has the advantages that (1) the preparation process is simple and controllable; (2) the stability of the nanoparticle is greatly improved, and the nanoparticle can be stabilized in a solution for up to 6 months; (3) a freeze-drying protective additive is not needed for freezing and drying; the obtained freeze-dried powder dissolved again shows small change on particle size and polydispersity index compared with freeze-dried powder without being dissolved; (4) the targeting for tumor cells is enhanced.

The invention discloses a multi-targeting antitumor composite preparation and a preparation method thereof. The multi-targeting antitumor composite preparation comprises a Fu vesicle suspension, DOX-BSA and FA-DOX. A vesicle administration system is prepared through integrating immunotherapy, positive targeting chemotherapy medicine drug molecules and a passive targeting administration system realize in order to realize low toxicity and high efficiency treatment of tumors, and the vesicle administration system can obviously slowly release Fu to effectively containment tumor development and has small influences on tumor bearing bodies; a newly synthesized immunogen can obtain an antibody with high sensitivity, so foundation is laid for formation of an immune compound macromolecule required by immunotherapy; and the micro-molecular compound FA-DOX has a molecule bridging effect in immunotherapy to promote formation of the immune compound macromolecule. The composite preparation has obviously better curative effects than simple particle administration chemical treatment or simple immunotherapy, has obviously low toxicity, can improve the antitumor effect, and is an anticancer composite preparation with wide research and application prospect.

The present invention provides an sgRNA specifically targeting a human DNMT1 gene based on a CRISPR / Cas9 system, a use thereof for guiding the CRISPR / Cas9 system in a drug for treatment and / or prevention of tumors, and a method correspondingly constructing a sgRNA expression vector. The CRISPR / Cas9 system can rapidly, efficiently and specifically knock out the human DNMT1 gene, induces inactivation of the DNMT1 gene in tumor cells, inhibits expression of the DNMT1 and methylation of DNA and thus inhibits tumor cell growth.

The invention relates to an antitumor drug combination and a preparation and application thereof. The drug combination comprises a tumor angiogenesis inhibitor compound and disulfiram. Weight ratio ofthe angiogenesis inhibitor compound to disulfiram is (1-15): (1-20). By combination of the tumor angiogenesis inhibitor compound and disulfiram for medication, the antitumor drug combination has thefunction of remarkably raising anticancer effect in comparison with any single ingredient. In addition, tumor inhibiting rate is raised by 20% and above. The data is a great progress in the research on cancer treatment. In the inventor's first research, it is found that the combination of the angiogenesis inhibitor and disulfiram at a specific ratio has a remarkable synergistic effect and the drugcombination has a good clinical application prospect.

The invention relates to biomimetic nanoparticles for gas production and anti-tumor activity catalyzed by bioenzymes and a preparation method thereof. Firstly, MnCO (Manganese carbonyl compounds) areloaded on a PLGA (polylactic acid-glycolic acid) solution, GOX (glucose oxidase) is embedded in the above mentioned system, a MnCO-PLGA nano solution embedded with GOX is prepared through volatilization of an emulsified solvent, GOX-MnCO-PLGA is squeezed into vesicles derived from erythrocyte membranes, and the biomimetic nanoparticles for gas production and anti-tumor activity catalyzed by bioenzymes. The prepared biomimetic nanoparticles provide a novel tumor treatment strategy, a gas therapy and a hunger therapy are synergistically used, and erythrocyte membranes serve as nanoparticle capsids, so that the prepared biomimetic nanoparticles can control formation of carbon monoxide in situ in tumor target tissues and have good anti-tumor effects.

The present invention is one kind of antitumor medicine and its production process, and belongs to the field of Chinese medicine technology. The antitumor medicine of the present invention has the material including wild buckwheat rhizome and Sarcandrae. The medicine of present invention can inhibit S180 caruncle obviously with tumor inhibiting rate raising with increased dosage and coinciding with the dose-effect relationship, has obtain Lewis lung cancer inhibiting effect, and has obvious immunity strengthening effect. Therefore, the present invention has high antitumor effect and low toxicity.

The invention discloses a chickpea sausage and a preparation method thereof. The chickpea sausage is prepared by using beef and chickpeas as raw materials, unfreezing, trimming and secondarily chopping the beef, using 2.5 percent of table salt, 0.03 percent of sodium nitrite and 0.3 percent of sodium hexametaphosphate for curing comminuted meat, adding 1.7 percent of edible spice, 0.3 percent of Kunlun chrysanthemum oil resin and 10 percent of modified starch, then performing secondary chopping, and selecting, soaking and cooking the chickpeas; mixing the treated beef with the chickpeas, adding 0.5 percent of carrageenan and 0.1 percent of gelatin for bonding, and performing steps of filling, baking, cooking, cooling and the like. The chickpea sausage is gentle in flavor, is fine and even in taste, is obvious in sense of meat, is good in elasticity, is moderate in stickiness and has a wide application value.

The invention discloses an irinotecan or irinotecan hydrochloride lipidosome and a preparation method thereof. The lipidosome contains irinotecan or irinotecan hydrochloride, neutral phospholipid and cholesterol, wherein the weight ratio of the cholesterol to the neutral phospholipid is 1:(3-5), and the irinotecan or irinotecan hydrochloride lipidosome is prepared by an ion gradient method.

The invention relates to a self-microemulsifying nanocombination comprising lucid ganoderma total triterpene and jobstears seed oil and a preparation method and application thereof. The lucid ganoderma total triterpene and the jobstears seed oil are adopted as medicinal materials, the jobstears seed oil doubles as the oil phase, the mixture of surfactant and cosurfactant is adopted as medicine carrier, and the parts by weight are as follows: 15 parts of lucid ganoderma total triterpene, 45 to 150 parts of jobstears seed oil, 45 to 200 parts of surfactant and 20 to 100 parts of cosurfactant. The invention also discloses the preparation method and application of the self-microemulsifying nanocombination. The invention prepares the self-microemulsifying nanocombination with the water-insoluble medicinal lucid ganoderma total triterpene and the jobstears seed oil, wherein the jobstears seed oil has the effects of both medicine and adjuvant, so that the dosage of preparation adjuvant, and moreover, the jobstears seed oil and the lucid ganoderma total triterpene which are compatible have a synergistic antitumor effect. Meanwhile, the self-microemulsifying nanocombination can promote the absorption of medicines, so that the antitumor effect can be enhanced.

This invention relates to endostatin small molecular polypeptide, its coding nucleotide sequence and complementary strand. The endostatin small molecular polypeptide has 30 amino acid residues corresponding to a nucleotide sequence with a full length of 90 bp and a complementary strand with a length of 88 bp. The endostatin small molecular polypeptide avoids the defects of the current endostatin such as high molecular weight, limit to subcutaneous injection and intramuscular injection, large dosage and unsafety in intravenous injection. The endostatin small molecular polypeptide has such advantages as low molecular weight and no medicine accumulation, and is suitable for intravenous, intramuscular and subcutaneous injection. The coding nucleotide sequence and complementary strand can be synthesized by an automatic synthesizer, and used to manufacture endistatin in large scale by genetic engineering after recombination and transformation.

The invention discloses a probiotic fermented traditional Chinese medicine compound composition for treating liver cancer and preparation and detection methods thereof. The preparation method includes: performing water extraction on traditional Chinese medicine compound medicinal materials; adding nutrient and inorganic salt different in proportion; sterilizing at high temperature, and performing compound intestinal tract probiotic fermentation; stabilizing to obtain the probiotic fermented traditional Chinese medicine compound composition. The traditional Chinese medicine compound medicinal materials include Radix bupleuri, Radix scutellariae, Radix ginseng rubra, Herba hyperici japonici, string stonecrop herb, roasted turtle shell, root of red-rooted salvia, selfheal, raw oyster, Rhizoma corydalis, Radix curcuma piece, polyporus umbellatus and Radix Glycyrrhizae Preparata.The invention further discloses an identification method and a detection method of the probiotic fermented traditional Chinese medicine compound composition. Experiments show that treatment effect of traditional Chinese medicine extract can be improved remarkably, tumor inhibition rate can be increased, and the probiotic fermented traditional Chinese medicine compound composition has remarkable effect on inhibiting liver tumor growth. Taste of the traditional Chinese medicine compound extract can be improved remarkably, irritation of the traditional Chinese medicine extract to intestines and stomach can be reduced, and appetite and physique of patients can be improved.

The invention relates to the field of drugs, in particular to a carbamidine derivative containing a furan structure and a composition of the carbamidine derivative. The derivative and the compositionthereof can be used for preparing drugs for treating a pathologic characteristic disease with an indoleamine 2,3-dioxygenase mediated tryptophan metabolic pathway. The invention further relates to a method for preparing the derivative and an intermediate thereof.

The invention discloses a preparation method of an abalone mask. The preparation method is characterized in that abalone hydrolysate is prepared from abalone serving as the raw material, and is matched with a traditional Chinese medicine extracting solution taking almonds, Wolfiporia extensa, cucumbers, mint, green tea, peach blossom and roses as the raw materials, then honey is added for mixing, a mask solution is prepared, and a mask sheet is soaked in the mask solution and is packaged in vacuum for preparation. The abalone hydrolysate, the traditional Chinese medicine extracting solution and the honey in the components jointly have a beautifying function, the components are compatible and supplement one another, can supplement skin nutrition, nurse the skin cells to be young and accelerate cell neogenesis, enables the skin to be tender, smooth and anti-aging, can improve local blood circulation and has better toxin expelling, anti-freckle, repairing and beauty maintaining effects.

The invention relates to an IL-12 and TNF-alpha autologous tumor vaccine which is suitable for tolerantable biopsy and operative patients. The vaccine is a composite containing a IL-12 gene, a TNF-alpha gene and a tumor cell, has the characteristics of simple operation, high immunogenicity and target ability, low preparation cost and convenience for clinical application, and is mainly used for anti-tumor immunotherapy which comprises the treatments of residual tumor after operation and unresectable tumor.

The invention discloses a dendritic cell tumor vaccine and a preparation method and application of the dendritic cell tumor vaccine, and belongs to medicine preparations containing antigens or antibodies. According to the dendritic cell tumor vaccine and the preparation method and application of the dendritic cell tumor vaccine, plasmid carrying a GPC3 gene and an eukayotic expression vector pcDNA-DEST53 are recombined, pGFP-GPC3 recombined plasmid is obtained, the recombined plasmid is led into immaturate dendritic cells in a nucleofector mode, the immature dendritic cells are promoted to become mature by cell factors TNE-alpha, and the dendritic cell tumor vaccine DC-GPC3 is obtained. After the obtained dendritic cell tumor vaccine and CIK cells are co-cultured, the tumor killing rate and the tumor restraining rate to high-expression GPC3 liver cancer cells are obviously increased, the good liver cancer resisting effect is presented, and the wide application prospect is achieved.

The invention relates to the field of tumor DNA (Deoxyribose Nucleic Acid) vaccines and virus vector vaccines, and in particular relates to recombinant DNA vectors VR-S8 and VR-MS, recombinant adenoviruses AD-S8 and Ad-MS and a recombinant poxvirus MVA-MS vaccine, as well as application of DNA vaccines and optimized combination of immunization ways between virus vector vaccines to preparation of antitumor vaccines.

The present invention belongs to the field of medicine, and specifically relates to a new borneol use and a lung cancer treatment drug composition. The first technical problem solved by the present invention is a use of borneol in anticancer drug preparation. The second technical problem solved by the present invention is to provide a completely new lung cancer treatment drug composition, which comprises borneol and cyclophosphamide. Test results show that single use of the borneol provides a lung cancer inhibition effect, combination of the borneol and the cyclophosphamide provides an increased tumor inhibition rate, and improvement of leukocyte inhibition by the cyclophosphamide is further provided. With the present invention, a completely new, safe and efficient selection is provided for clinical lung cancer treatment.

The invention discloses a traditional Chinese medicine composition and its application as a radiotherapy sensitizer, and belongs to the field of traditional Chinese medicine. Since no quite effective pharmacotherapy is provided for solid tumors currently, a first purpose of the invention is to provide a traditional Chinese medicine composition for treating solid tumors, which comprises the following components: 5-10 parts of toad skin, 6-8 parts of mylabris, 5-10 parts of panax notoginseng, 10-15 parts of leeches, 20-30 parts of curcuma rhizome, 20-30 parts of ground beetles, 5-10 parts of pangolins, 10-15 parts of radix astragali, 20-30 parts of coix seeds, 5-15 parts of angelica, and 5-15 parts of licorice. The traditional Chinese medicine composition has very good anticancer activity with respect to solid tumors such as lung cancer and the like, and can be used as a radiotherapy sensitizer.

The present invention provides an antitumor prodrug, activator thereof, composition thereof and use thereof, the activator being shown as formula (I). The antitumor prodrug in the present invention has unexpectedly high selectivity in the treatment of tumor, and greatly reduces the harm of released cytotoxic drug on the human body.