Dendritic cell tumor vaccine and preparation method and application thereof

A technology for dendritic cell and tumor vaccine, which is applied in the field of dendritic cell tumor vaccine and its preparation, and can solve problems such as affecting the therapeutic effect.

Inactive Publication Date: 2015-04-22
天津市第一中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, when applying DC tumor vaccines, complex immunosuppression in tumor patients limits

Method used

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  • Dendritic cell tumor vaccine and preparation method and application thereof
  • Dendritic cell tumor vaccine and preparation method and application thereof
  • Dendritic cell tumor vaccine and preparation method and application thereof

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[0026] The present invention will be described in detail below with reference to the accompanying drawings and embodiments.

[0027] 1. Materials, reagents and instruments

[0028] 1.1 Materials and reagents

[0029] pDONR223-GPC3 plasmid: purchased from Immunosoft Biological Company;

[0030] pcDNA-DEST53 eukaryotic expression vector: purchased from Invitrogen Biological Company;

[0031] Recombinase: purchased from Invitrogen Biological Company;

[0032] Top 10 E. Coli: purchased from Merck Bio;

[0033] Ampicillin: purchased from Shanghai Sangon Biological Company;

[0034] Plasmid mini-extraction kit: purchased from Tiangen Biochemical Technology Co., Ltd.;

[0035] Lymphocyte separation solution: purchased from Tianjin Haoyang Biological Products Technology Company;

[0036] GM-CSF: purchased from BD Pharmingen Biological Company;

[0037] IL-4: purchased from BD Pharmingen Biological Company;

[0038] Digestive fluid components: purchased from GibcoBRL Biological...

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Abstract

The invention discloses a dendritic cell tumor vaccine and a preparation method and application of the dendritic cell tumor vaccine, and belongs to medicine preparations containing antigens or antibodies. According to the dendritic cell tumor vaccine and the preparation method and application of the dendritic cell tumor vaccine, plasmid carrying a GPC3 gene and an eukayotic expression vector pcDNA-DEST53 are recombined, pGFP-GPC3 recombined plasmid is obtained, the recombined plasmid is led into immaturate dendritic cells in a nucleofector mode, the immature dendritic cells are promoted to become mature by cell factors TNE-alpha, and the dendritic cell tumor vaccine DC-GPC3 is obtained. After the obtained dendritic cell tumor vaccine and CIK cells are co-cultured, the tumor killing rate and the tumor restraining rate to high-expression GPC3 liver cancer cells are obviously increased, the good liver cancer resisting effect is presented, and the wide application prospect is achieved.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations containing antigens or antibodies, in particular to a dendritic cell tumor vaccine and its preparation method and application. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world, with high morbidity and mortality, and about 620,000 patients are newly diagnosed with HCC every year worldwide. Due to the high rate of chronic hepatitis B virus infection in the Chinese population, my country accounts for 55% of the annual new cases in the world, becoming one of the regions with the highest incidence of liver cancer in the world. Liver transplantation is considered to be a potential curative method for liver cancer, and its 5-year overall survival rate has reached 60-80%. However, due to potential tumor micrometastases before transplantation, tumor implantation during surgery, and the application of immunosuppressants after tra...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P35/00
Inventor 王玉亮刘涛穆红江雁
Owner 天津市第一中心医院
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