Dendritic cell tumor vaccine and preparation method and application thereof
A technology for dendritic cell and tumor vaccine, which is applied in the field of dendritic cell tumor vaccine and its preparation, and can solve problems such as affecting the therapeutic effect.
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[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.
[0027] 1. Materials, Reagents and Instruments
[0028] 1.1 Materials and reagents
[0029] PDONR223-GPC3 plasmid: purchased from Immunosoft Biological Company;
[0030] pcDNA-DEST53 eukaryotic expression vector: purchased from Invitrogen Biological Company;
[0031] Recombinase: purchased from Invitrogen Biological Company;
[0032] Top 10 E. Coli: purchased from Merck Biological Company;
[0033] Ampicillin: purchased from Shanghai Sangon Biological Company;
[0034] Plasmid small extraction kit: purchased from Tiangen Biochemical Technology Co., Ltd.;
[0035] Lymphocyte separation liquid: purchased from Tianjin Haoyang Biological Products Technology Company;
[0036] GM-CSF: purchased from BD Pharmingen Biological Company;
[0037] IL-4: purchased from BD Pharmingen Biological Company;
[0038] Digestive juice components: purchased from Gib...
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