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IL-12 and TNF-alpha autologous tumor vaccine

A tumor vaccine, autologous technology, applied in the field of biomedicine, can solve the problems of poor safety, weak immunogenicity, and large side effects

Inactive Publication Date: 2010-06-02
尹晓玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing vaccines show significant anti-tumor potential, they also have disadvantages such as poor specificity, weak immunogenicity, large side effects, and poor safety. Small tumor vaccines are a major issue facing the tumor community

Method used

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  • IL-12 and TNF-alpha autologous tumor vaccine
  • IL-12 and TNF-alpha autologous tumor vaccine
  • IL-12 and TNF-alpha autologous tumor vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1. Preparation of tumor vaccines expressing IL-12 and TNF-α genes

[0024] 1. Isolation of tumor cells: put the resected tumor specimens into physiological saline containing 50 μg / ml gentamicin, and quickly send them to the laboratory under sterile conditions. Remove necrotic tissue, fat and normal tissue in the ultra-clean bench, wash the removed viscera three times with sterilized PBS solution, and then carefully cut the tissue repeatedly with ophthalmic surgical scissors until the size is 1mm 3 The left and right small pieces were washed with PBS until the tissue pieces turned white. Transfer it to a sterile centrifuge tube and let it stand for a few minutes to allow the tissue pieces to settle to the bottom of the tube naturally, then discard the supernatant. Take 1ml of 0.25% trypsin-0.02% EDTA mixed digestion solution, add it to a centrifuge tube, mix it with the tissue block, add a stopper, digest in a 37°C water bath for 8-10 minutes, shake the test tube every ...

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Abstract

The invention relates to an IL-12 and TNF-alpha autologous tumor vaccine which is suitable for tolerantable biopsy and operative patients. The vaccine is a composite containing a IL-12 gene, a TNF-alpha gene and a tumor cell, has the characteristics of simple operation, high immunogenicity and target ability, low preparation cost and convenience for clinical application, and is mainly used for anti-tumor immunotherapy which comprises the treatments of residual tumor after operation and unresectable tumor.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an IL-12 and TNF-α autologous tumor vaccine suitable for patients who can tolerate biopsy and operation. Background technique [0002] Malignant tumors are one of the major diseases that threaten human health and life, and their incidence is increasing year by year, and many tumors cannot be cured by conventional methods such as surgery, radiotherapy, and chemotherapy. With the in-depth study of the molecular mechanism of tumorigenesis and development and the rapid development of biotechnology, biological therapy has become the fourth mode of comprehensive tumor treatment, and has received more and more attention. Tumor biotherapy (Biotherapy) is a new treatment for tumor prevention and treatment using modern biotechnology and its products. Tumor effects mainly include somatic cell therapy and cytokine therapy, tumor vaccines, molecular targeted therapy, radioimmunotargeted ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K48/00A61P35/00
Inventor 尹晓玲林海波
Owner 尹晓玲
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