Preparation method of bone mesenchymal stem cell carrying NK4 gene and application thereof

Abstract

The invention discloses a preparation method of bone mesenchymal stem cells carrying NK4 genes and application thereof in medicine for treating gastric cancer. The preparation method comprises the following steps: extracting the NK4 genes from an HGF plasmid, using an in-Fusion technology to directionally clone the NK4 genes to a slow virus expression carrier plasmid, constructing an NK4-EGFP fusion gene recombinant slow virus carrier plasmid (pGC-FU-NK4) and then co-transfecting a 293T cell with the pGC-FU-NK4, a pHelper1.0 carrier and a pHelper2.0 carrier, packaging to produce NK4 overexpression slow virus particles (Lenti-NK4), using slow virus (Lenti-NK4) carrying NK4-EGFP fusion gene to transfect the bone mesenchymal stem cells to establish in vitro bone mesenchymal stem cells to stably express the NK4 genes. Balb / C nude mice animal experiments prove that the NK4 gene therapy taking BMSCs as a carrier can obviously inhibit the growth of subcutaneous transplant tumor of gastric cancer with high tumor inhibition efficiency, and the NK4 gene therapy is a good gene therapeutic method for gastric cancer.

Description

technical field [0001] The present invention relates to the field of medical inventions, in particular to a method for preparing bone marrow mesenchymal stem cells carrying NK4 gene and the application of bone marrow mesenchymal stem cells carrying NK4 gene in drugs for treating gastric cancer. Background technique [0002] The annual prevalence and mortality of gastric cancer in my country are more than twice the world average, and most patients are already in the advanced stage when they are diagnosed. Although the treatment effect of early gastric cancer is relatively optimistic, for advanced gastric cancer, most of them have lymphatic and hematogenous metastasis, and a considerable number of patients have lost the opportunity for surgery when they are diagnosed. Although chemotherapy and comprehensive treatment strategies have improved the curative effect, the overall curative effect Poor. Therefore, it is necessary to explore new treatment methods. [0003] Tumor gene...

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Problems solved by technology

It solves the problem of low transfection rate of introducing NK4 gene into mesenchymal stem cells using adenovirus as a vector

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