Active material of polypeptide proteins in bloody clam, preparation method and usage

Abstract

The invention relates to multipiptide protein active substance in a kind of bloody clam (Arca subcrenata Lischke; Scapharca subcrenata), the prepn. method and the uses. The prepn. method is that bloody clam is homogenated to obtain superantant which is treated by chromatography in ion exchange column, desalt, chromatograph separation in gel colum to be combined to several peaks with molecular weight 3-15 KDa which is concentrated and dried to obtain multipeptide protein active substance. Said active substance has 73.72 percent of cancer-inhibiting ratio and can be made as anti-cancer medicine.

Description

Technical field:

[0001] The invention discloses a polypeptide protein active substance in clams (Arca subcrenata Lischke; Scapharca subcrenata;), a preparation method and application thereof. Background technique:

[0002] Hairy clams (Arca subcrenata Lischke; Scapharca subcrenata;) belong to the phylum Molluscs, Phyllobranches, Order Dendontia, and Scapharca family. Its shell is Chinese medicine corrugated seed. There are 31 to 34 radial ribs. The surface of the shell is hairy and brown in color. Produced in the northern coast of my country.

[0003] "China Marine Medicine" magazine, 2003, No. 2 P37, introduced the separation method of oyster protein. Fresh oyster soft body, homogenate with 2 times the amount of normal saline at 4°C, centrifuge at 3000r / min for 15min, take the supernatant, add (NH) 2 SO 4 , so that the solution's (NH) 2 SO 4 The saturations reached 50%, 75% and 100%, respectively, and stood at 4°C for 12h. Then centrifuge at 3000r / min for 15min, ta...