64 results about "Acid sensitivity" patented technology

The invention relates to a dual sensitive amphiphilic triblock copolymer containing disulfide bond and acylhydrazone bond and a preparation method and an application of the dual sensitive amphiphilic triblock copolymer. A BAB type amphiphilic triblock copolymer consists of a hydrophobic polyester block A and a hydrophilia polyethylene glycol block B, wherein the block A is polyether containing active terminal amino group and disulfide bond; the block B is benzaldehyde ester of an ethylene glycol monomethyl ether or single terminated polyoxyethylene polypropylene oxide copolymer; the block A and the block B are connected through acid sensitive acylhydrazone bond generated from reaction of active terminal amino group and aldehyde group. The dual sensitive amphiphilic triblock copolymer is good in biocompatibility and biodegradability, can be formed into nanoparticles with oxidation reduction sensitivity and acid sensitivity in a water medium in a self-assembling manner, can wrap hydrophobic medicines to form nano medicine preparations, and can promote rapid release of medicines in tumor cells due to fracture of disulfide bond and acylhydrazone bond which are sensitive to oxidation reduction in a high concentration glutathione and acid micro environment in the tumor cells.

The present invention provides an acid sensitive doxorubicin prodrug based on polyethylene glycol, and a method and an application of the acid sensitive doxorubicin prodrug based on polyethylene glycol. The preparation method comprises: preparing a polyethylene glycol whose end contains an acid sensitive acetal group and a p-nitrophenyl ester azide group by a reaction of a polyethylene glycol whose double ends contain diazido ethyl diacetal groups with a p-nitro phenyl propargyl carbonate; preparing the acid sensitive doxorubicin prodrug based on the polyethylene glycol by the reaction of the prepared polyethylene glycol with doxorubicin hydrochloride. The acid sensitive and water soluble doxorubicin prodrug has good biocompatibility and acid sensitivity, thereby being used as a stimulation sensitive antineoplastic prodrug.

The invention provides a tumor active-targeting nano drug delivery system capable of reversing drug-resistance. The drug delivery system is polylactic-co-glycolic acid (PLGA) nanoparticles mixed with lipid, and the nanoparticle core is PLGA. Drug-loaded nanoparticles are prepared as follows: inserting AA-polyethylene glycol-cholesterol linkers and triphenylphosphine-cholesterol linkers in the surface of PLGA; encapsulating DOX into the PLGA nanoparticles mixed with lipid to obtain the drug-loaded nanoparticles. The drug delivery system has the acid sensitivity, which enables the drug delivery system to proactively recognize tumor cells and position and release DOX into the mitochondria and cell nuclei of tumor cells, thereby enhancing the anti-tumor activity, reversing the drug-resistance of the tumor and providing a new strategy for improving the efficacy of DOX.

The invention relates to a medicinal preparation, particularly relates to a targeting preparation for resisting drug-resistant tumor, as well as a preparation method and application thereof, and belongs to the technical field of pharmaceuticals. The targeting preparation for resisting drug-resistant tumor comprises a part A which is gold nanoparticles, a part B which is polyethylene glycol, a part C which is a hydrazone bond, and a part D which is a chemotherapeutic drug. The targeting gold nanoparticle drug delivery system prepared by linking amycin and gold nanoparticles via hydrazone bond has acid sensitivity, and can specifically release amycin to an environment with pH less than 6 to realize specific response to microenvironment (lysosome and endosome, pH less than 6) in drug-resistant tumor. The targeting preparation provided by the invention meets the needs for drug-resistant tumor therapy and can be used for reducing toxic and adverse side effects of amycin.

The invention provides an acid sensitivity lactobacillus bulgaricus strain and the usage thereof, belonging to the biological engineering technology field. An acid sensitivity strain L.B-FM-6 which is a lactobacillus delbrueckii subsp. bulgaricus is separated and screened in the research of the natural dairy ferment-tibet kefir. The research on the storage stability of the fermented dairy product, fermented juice and the fermented vegetable juice which are fermented by the combination of the strain L.B-FM-6 and other microbial strains solves the defects of short shelf life of the active lactobacillus drink and displays the superiority of the strains in fermenting the dairy product and the juice.

The invention discloses a preparation method of a tumor-targeted acid-sensitivity prodrug-magnetic nanoparticle conjugate and an application thereof. The method comprises the following steps: preparing a tumor-targeted acid-sensitivity prodrug from raw materials such as hyaluronic acid and adriacin doxorubicin; preparing an amino-containing magnetic nanoparticle through a coprecipitation method and modification of an amino silane coupling agent; and finally carrying out chemical couplong on the hyaluronic acid tumor-targeted acid-sensitivity prodrug and the magnetic nanoparticle through amidation reaction. The tumor-targeted acid-sensitivity prodrug-magnetic nanoparticle conjugate prepared by the method has good water dispersion stability; the superparamagnetism of the magnetic nanoparticle is not affected; long cycle of the magnetic nanoparticle in vivo is facilitated; moreover, the nanoparticle is also coupled to an anti-tumor medicine adriacin doxorubicin, so as to be effectively released in weak-acidity parts such as tumor cells; and the preparation method is expected to be applied to the fields such as diagnosis and treatment of tumors.

The invention discloses a polyethylene glycol-aminated poly(glycidyl methacrylate)-poly(diisopropylaminoethanol methacrylate) triblock polycation and a synthetic method thereof, and a dual acid-sensitivity multilayer cation micelle prepared from the triblock polycation and a preparation method thereof. The cation micelle comprises a neutral polyethylene glycol PEG external layer, an aminated poly(glycidyl methacrylate) PAG intermediate layer and a poly(diisopropylaminoethanol methacrylate) PDPA inner layer, wherein the PEG external layer can shield a part of positive charges and improve stability of the micelle, the PAG intermediate layer has positive electricity and can absorb electronegative nucleic acid drugs, and the PDPA inner layer has hydrophobicity and can entrap hydrophobic drugs. Furthermore, the invention also discloses application of the dual acid-sensitivity multilayer cation micelle in synchronous co-delivery of nucleic acid and hydrophobic anticancer drugs, and the dual acid-sensitivity multilayer cation micelle is mainly used for reversion of multidrug resistance of cancer cells or inhibition of cancer cell metastasis.

The invention discloses an miRNA-supported composite nanoparticle. The miRNA-supported composite nanoparticle is prepared from a cation targeted membrane material, an acid sensitivity membrane material and at least one miRNA, wherein the cation targeted membrane material is selected from a galactose-polylysine-polycysteine polymer; the acid sensitivity membrane material is selected from a polyethylene glycol-polylysine polymer of which a side chain is modified by 3,4,5,6-tetrahydrophthalic anhydride ligand; and the miRNA is selected from miRNA for inducing differentiation from tumor-associatedmacrophage to M1 type macrophage. According to the miRNA-supported composite nanoparticle disclosed by the invention, accurate delivery of the tumor-associated macrophage is realized by pH value targeting and active targeting, and the function of promoting the differentiation from the tumor-associated macrophage to the M1 type macrophage is realized.

Provided are photoresist compositions useful in forming photolithographic patterns by a negative tone development process. Also provided are methods of forming photolithographic patterns by a negative tone development process and substrates coated with the photoresist compositions. The compositions, methods and coated substrates find particular applicability in the manufacture of semiconductor devices. The photoresist compositions are provided, comprising a first polymer of acid sensitivity, a second polymer formed by a monomer of the following formula 1 which is not of acid sensitivity and not containing fluorine or silicon, a photoinduced acid generation agent and a solvent. Besides, the surface energy of the second polymer is lower than that of the first polymer.

The invention provides a preparation method of a drug controlled release nano-system sensitive to tumor microenvironment, the drug controlled release nano-system sensitive to tumor microenvironment prepared by the preparation method and an application of the drug controlled release nano-system for treating cancer. The drug controlled release nano-system is sensitive to both reduction and weak acid, and has good biocompatibility and cancer treatment effect.

Compositions and methods of making a modified polyhydroxylated polymer comprising a polyhydroxylated polymer having reversibly modified hydroxyl groups, whereby the hydroxyl groups are modified by an acid-catalyzed reaction between a polydroxylated polymer and a reagent such as acetals, aldehydes, vinyl ethers and ketones such that the modified polyhydroxylated polymers become insoluble in water but freely soluble in common organic solvents allowing for the facile preparation of acid-sensitive materials. Materials made from these polymers can be made to degrade in a pH-dependent manner. Both hydrophobic and hydrophilic cargoes were successfully loaded into particles made from the present polymers using single and double emulsion techniques, respectively. Due to its ease of preparation, processability, pH-sensitivity, and biocompatibility, of the present modified polyhydroxylated polymers should find use in numerous drug delivery applications.

The invention discloses a pH-sensitive amphipathic graft copolymer POEAd-g-MPEG, a preparation method and an application of the graft copolymer. The copolymer has acid sensitivity, biocompatibility and biodegradability, can self-organize in an aqueous solution, and forms a drug carrier with a shell-core structure. The copolymer has bright application prospects in the field of tumor treatment.

The invention relates to a preparation method of drilling core containing acid liquor indicator, which comprises the following components as calculated by the total weight 100% of the drilling core: 14% of epoxy resin, 2% of quadrol, 2% of acetone and 1% of methyl red. The preparation method has the following steps: mixing the above components; weighting the rock waste raw materal accounting for 14% of the total weight of the drilling core, and washing an performing oil treatment on the raw material by petroleum ether; drying for 3h under the temperature of 95 DEG C, cooling and adding 1% crystal violet; stirring a cementing agent with the rock waste raw material evenly, placing the mixture in a die and pressurizing by 7MPa-10MPa for 12h; and dismantling the die, drying the drilling core for 6h at the temperature of 95 DEG C, and naturally cooling to room temperature. The permeability, water sensitivity, salt sensitivity, velocity sensitivity, acid sensitivity and alkali sensitivity of the drilling core in the invention are similar to those of the natural core, which is proved by a stepped permeability tester and five-sensitivity tests; and the drilling core has good indicating effect on acid liquor.

The present invention belongs to the technical fields of biomedical medicine and nano-medicine, and relates to a stem cell tumor targeting system with internal nano prodrug and a preparation method thereof. The method uses stem cells as cell carriers to prepare an anti-tumor targeting system with general formula of Stem cells-(RGD-PPCD)n through endocytosis and inner load of nano prodrug. The nano prodrug has the characteristics of targeting, sustained release and acid sensitivity release; and the stem cell carrier can remotely target primary lesion and metastasis of tumor. In vitro experimental results show that the stem cells after drug load can retain the proliferation capacity and tumor migration characteristics; and the constructed system is stable to ensure that the drug stays in the form of nano-prodrug for several days in the cells, and can slowly release the drug. In vivo experimental results show that the constructed stem cell targeting system can significantly prolong the survival time of tumor-bearing animals and can maintain normal neurobehavioral characteristics of tumor-bearing animals in comparison with the original drug and nano prodrugs, so as to play the efficient, secure and long-lasting anti-tumor effect.

The invention relates to a semi-solid state acid-sensitive amphiphilic block copolymer for solubilizing a hydrophobic drug, and a preparation method and application thereof. The invention relates to asynthesis method and application of a semi-solid state acid-sensitive amphiphilic block copolymer (PCL-PA-PEG) prepared through simple polycondensation of polyethylene glycol (PEG), a divinyl ether compound and polycaprolactone glycol. The copolymer prepared by the preparation method is high in acid sensitivity, biocompatibility and biodegradability, is very convenient for solubilizing the hydrophobic drug to form a partial drug slow-release preparation or a cyclic dosing preparation, has a great research value and application prospect in the aspect of drug delivery, targeted treatment and diagnosis, and also can be blended with a hydrophilic drug to form a drug delivery carrier capable of realizing directly partial controlled-release dosing or cyclic injection dosing so as to release anactivator such as a polypeptide drug in a controlled manner.

The invention relates to the damage evaluation field of working fluid on the reservoir stratum in reservoir stratum reconstruction and reservoir stratum protection processes in gas reservoir exploitation of shale, and especially relates to a method for testing acid sensitivity of shale reservoir stratum. In an industry standard method testing process, the method takes liquid as an experiment medium, the gas penetration capability is an important parameter for influencing the gas well productivity, the above method accords with a practical gas layer production case; when the liquid sensitivity of shale is evaluated by an industry standard method, due to low liquid permeability, flowing speed of liquid in rock core is slow, time for measuring liquid flow amount through the rock core is long, in the method provided in the invention, liquid flow amount through the rock core is not required for measuring, time is saved; when the liquid sensitivity of shale is evaluated by the industry standard method, liquid flow amount through the rock core is hardly measured, large error is generated, liquid flow amount through the rock core is not required for measuring in the invention provided in the method, and testing error is reduced.

The invention relates to a function enhanced metal organic framework-based composite phase change material and a preparation method thereof. The preparation method comprises the following steps: mixing a metal organic framework material, copper nitrate, an organic ligand and silver nitrate to form a precursor solution, heating the precursor solution to a certain temperature and performing heat preservation and carrying out a reaction for a period of time, performing cooling to room temperature after the reaction is completed, performing filtering, sequentially performing washing, centrifugal separation, drying on a first filter residue obtained by filtration and then performing dispersion in an acid solution, performing stirring for a period of time, performing filtering, sequentially performing washing and drying on a second filter residue obtained by filtration and then performing dispersion in a second organic solvent, performing stirring under ultraviolet irradiation for a period of time, performing filtering, performing washing and drying on a third filter residue obtained by filtration to obtain a metal organic framework, and compounding an organic phase change core materialwith the metal organic framework to obtain the composite phase change material. The acid sensitivity of the metal organic framework material is utilized to remove the metal organic framework materialthrough the acid solution, and thereby an adjustable pore size is realized.

The invention discloses a discharge adding agent for fracturing and acidizing, which can ensure the effective discharge of a fracturing fluid and has a water-lock prevention effect. The discharge adding agent is prepared from the following raw materials in parts by weight: 10-20 parts of isopropanol, 30-40 parts of potassium chloride, 30-40 parts of sodium chloride, 0.005-0.02 part of perfluoroheptyl sodium and 20-30 parts of erucamidopropyl sulfobetaine. When being used for fracturing and acidizing oil and gas wells, the discharge adding agent is diluted by water, and then heated to the temperature of 45 DEG C and added into a construction fluid. By using the discharge adding agent disclosed by the invention, the discharge of the working fluid of a well in operation and the protection of an oil reservoir can be realized at the same time. The discharge adding agent has the characteristic of uniform distribution of the working fluid and the effect of acid sensitivity resistance and is easy and convenient to use. The construction times can be reduced due to the adoption of the discharge adding agent, thus the discharge adding agent can be used widely.

The invention discloses a monomolecular resin based on a 1,4-disubstituted pillar[5]arene derivative. The monomolecular resin has a structure as shown in a formula which is described the specification. In the formula, the substituents R and R' are hydrogen atoms or acid-sensitive substituents respectively, and R and R' cannot be hydrogen atoms at the same time. The monomolecular resin based on the1,4-disubstituted pillar[5]arene derivative is simple in synthesis process and high in yield; intermediates and the product are easy to separate and purify during synthesis; the monomolecular resin is suitable for industrial production; the monomolecular resin can effectively inhibit the crystallization of molecules and are easy for film formation; and the monomolecular resin has the characteristics of good rigid structure, high glass transition temperature and good thermal stability and can well meet the requirements of photolithography process. The monomolecular resin of the invention can be used as a photoresist main body material and cooperated with other additives for preparation of a positive photoresist. Photoresist films of different thicknesses can be prepared through spin coating of the photoresist.

The invention discloses a preparation method of a Fe3O4 surface double-modified adriamycin targeted drug. The preparation method comprises the following steps: firstly by taking FeCl2.4H2O, FeCl3.6H2O and citric acid as raw materials, reacting and drying to obtain citric acid modified Fe3O4 nano particles by taking ammonium hydroxide, hydrazine hydrate and water as a reaction medium, and then reacting with dicarboxylate polyethylene glycol under the effect of a coupling agent and hydrazine hydrate, drying to obtain the citric acid/polyethylene glycol double-modified ferroferric oxide nano particles, reacting with adriamycin in a methanol solution, drying to obtain the Fe3O4 surface double-modified adriamycin targeted drug. The invention provides the preparation method of Fe3O4 surface double-modified adriamycin targeted drug with the drug sustained release behavior in good superparamagnetism and acid sensitivity.

The invention relates to a nitration method having the following principles: a phosgene species is converted with two equivalent silver nitrates into a double-mixed anhydride of carbonic acid and nitric acid, known here as carbonic acid dinitrate (I). Said operation is carried out in situ, and the formed dinitrate decomposes spontaneously. In addition to carbon dioxide, nitrate ions and nitronium ions are formed, said ions comprising electrophiles which are necessary for nitration. The solution which is used is acetonitrile, and is insignificant if the alcohol species is dissolved or suspended. The necessary equivalent silver nitrates are introduced into the system and optionally heated or cooled to the desired temperature. Subsequently, the acid chloride is introduced slowly, drop by drop or slowly little by little. Phosgene, diphosgene, triphosgene and chloroformic acid ester can be used as carbonic acid dichloride and monochloride, and their thiocarbonic acid analogues. A brown colouration and precipitated silver chloride display the formation of the carbonic acid reactants, said brown colouration rapidly discolouring due to an immediate reaction of the nitronium ions with the substrate with is to be nitrated. Towards the end of the addition of phosgene, the brown colouration remains for longer and longer until it no longer disappears. Then, it is stirred for another hour at room temperature. In the event of high acid-sensitive educts, non-nucleophilic nitrogen bases such as DBU can be added to the system in order to intercept the formation of nitric acid.

The invention discloses a preparation method of a degradable acid-sensitive polyaspartamide copolymer and a use of the degradable acid-sensitive polyaspartamide copolymer as a drug and a gene co-transmission nanoparticle. Hydrophilic and hydrophobic sections of the degradable acid-sensitive polyaspartamide copolymer respectively comprise aziridine-grafted polyaspartic acid and acetalated dextran, and a molecular weight ratio of the aziridine-grafted polyaspartic acid to acetalated dextran is 1: 1-10. The preparation method comprises that dextran undergoes an acetalation reaction, the reaction product is bonded to nitrine-terminated poly(b-benzyl-L-aspartate), through a benzyl ester aminolysis reaction, and the combined product is bonded to polyaziridine so that an acetalated dextran-polyaziridine-grafted polyaspartic acid derivative with acid sensitivity and degradability is prepared. The copolymer can form nanoparticles with uniform particle sizes and stability by a double emulsification method, and can be combined with a carrying drug and a gene and realizes targeting conveying to tumor cells so that tumor chemotherapy and gene treatment synergism is obtained. The copolymer has a wide application prospect in the field of tumor treatment.

The invention relates to a poly(2-diisopropylaminoethyl methacrylate) modified beta-cyclodextrin and aminated poly(glycidyl methacrylate) modified adamantane diblock host-guest polycation inclusion complex, a synthetic method thereof, a cationic micelle which is prepared from the above polycation inclusion complex and possesses dual acid sensitivities and a preparation method for the cationic micelle. Due to the fact that the cationic micelle possesses the dual acid sensitivities, the hydrophilic external layer of the micelle is capable of combining with proton and showing positive electricity under a neutral physiological condition that pH is 7.4, and the hydrophobic internal layer of the micelle is capable of combining with proton and being dissociated in a weakly acidic environment with pH lower than 6.3. The average hydrodynamics particle size of the cationic micelle is preferably 20-200 nm. The invention also relates to application of the cationic micelle with dual acid sensitivities to nucleic acid medicament conveying, and the cationic micelle is mainly applied to reverse the multidrug resistance of cancer cells or inhibiting cancer cell transfer.

The invention relates to a strength sensitivity evaluation method for a shale reservoir. The intensity sensitivity evaluation method comprises the following steps: measuring the compressive strength of the core after executing a standard process so as to obtain a first compressive strength; measuring the compressive strength of the core after executing a specified process so as to obtain second compressive strength; and obtaining a strength sensitivity index of the core by virtue of the first compressive strength and the second compressive strength, and evaluating the sensitivity of the shalereservoir by utilizing the strength sensitivity index. According to the sensitivity evaluation method disclosed by the invention, the limitation of the existing sensitivity evaluation is overcome, thesensitivity performances of the shale reservoir, such as water sensitivity, acid sensitivity, alkali sensitivity, stress sensitivity, temperature sensitivity and the like can be evaluated from the mechanical property, formation damage and collapse caused by operations such as hydraulic fracturing, acid squeezing, squeeze water throughput, waterflooding displacement and the like can be avoided, and a basis can be provided for optimizing the construction process parameters.