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Ganoderma tsugae active substance, preparation method thereof and composition thereof

An active substance, the technology of pine fir ganoderma lucidum, applied in the field of pine fir ganoderma lucidum active substances, can solve the problem of antioxidant properties or other active ingredients are rarely understood, and achieve the effect of preventing atherosclerosis

Active Publication Date: 2010-12-08
SYNGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the antioxidative properties or other active components of exopolysaccharides produced by liquid fermentation of Ganoderma lucidum

Method used

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  • Ganoderma tsugae active substance, preparation method thereof and composition thereof
  • Ganoderma tsugae active substance, preparation method thereof and composition thereof
  • Ganoderma tsugae active substance, preparation method thereof and composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 pine fir Ganoderma lucidum fermented liquid

[0030] 1.1 Sources of Pinus fir Ganoderma lucidum

[0031] The pine fir Ganoderma lucidum used in this embodiment is obtained from the China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center, CGMCC), the deposit number is CGMCC No.2861, and the deposit date is January 8, 2009. Day, but the active substances of Ganoderma lucidum described in the present invention are not limited to those obtained from this strain.

[0032] 1.2 Cultivation of pine and fir Ganoderma lucidum:

[0033] Ganoderma lucidum was inoculated on potato dextrose agar medium (Potato Dextrose Agar, PDA, Difco TM , REF213400) on a petri dish, cultured at 20-35°C for 5-10 days, cut it into pieces and inoculated it into potato dextrose culture solution (Potato Dextrose Broth, PDB, Difco TM, REF254920) in a 3L fluted shaker flask, cultured for 5-10 days under the conditi...

Embodiment 2

[0038] Example 2 The preparation, composition and biological activity analysis of active substances of Ganoderma lucidum

[0039] 2.1 Materials

[0040] The primary antibody to heme oxidase type 1 (HO-1) was purchased from Stressgen Biotechnologies (SB, San Diego, CA, USA). The antibody against transcription factor Nrf2 was purchased from Santa Cruz Biotechnology (Santa Cruz, California, USA). Anti-rabbit and anti-mouse antibodies conjugated with peroxidase were purchased from Amersham (Arlington Heights, IL, USA). All other reagents or materials were purchased from Sigma Corporation (St. Louis, MO, USA), or other readily available commercial sources.

[0041] 2.2 Method

[0042] (1) Endothelial cell culture

[0043] Bovine aortic endothelial cells (BAECs) were cultured in DMEM medium (Dulbecco's modified Eagle's medium, Invitrogen Company), and added 10% fetal bovine serum (fetal bovine serum, FBS, Invitrogen Company) and suitable concentrations of antibiotic. Cells wer...

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Abstract

The invention relates to a ganoderma tsugae active substance, produced by carrying out the following steps on ganoderma tsugae: (1) ganoderma tsugae is subject to liquid fermentation culture; (2) the fermentation filtration is extracted by ethyl alcohol, so as to obtain ethyl alcohol extraction sediment; (3) the obtained ethyl alcohol extraction sediment is subject to purification by colloid filtering chromatography, so as to obtain colloid filtering chromatographic product; (4) the obtained colloid filtering chromatographic product is extracted by acetone, and the obtained supernatant is the ganoderma tsugae active substance namely. The invention also provides ganoderma tsugae composition produced by the steps, the composition can be used for inducing or increasing the quantity of transcription factor Nrf2 in nucleus, improving the activity of antioxidation reaction segment promoter, improving the activity of sulphuric oxidoreduction protein enzyme promoter and inducing first type heme oxygenase and first type sulphuric oxidoreduction protein enzyme to express, so as to achieve the effect of cytoprotection or preventing arteriosclerosis.

Description

technical field [0001] The invention relates to an active substance of pine fir ganoderma, in particular to an active substance of pine fir ganoderma which can prevent atherosclerosis, its preparation method and its composition. Background technique [0002] For thousands of years, in Eastern countries, plants of the Ganodermataceae family have been proven to have medicinal effects, and the antioxidant effect of Ganoderma lucidum is one of its medicinal effects. Ganoderma tsugae, a flattened, porous mushroom of the genus Ganoderma lucidum, is also a cultivar used as a dietary supplement in Taiwan. In recent years, the fruiting bodies and mycelium extracts of solid-state cultured Ganoderma lucidum have been reported to have immunomodulatory, anti-cancer, anti-inflammatory and anti-oxidative effects. However, little is known about the anti-oxidant properties or other active components of exopolysaccharides produced by liquid fermentation of Ganoderma lucidum. [0003] Oxidat...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61P9/10
Inventor 魏钰珊谢佳雯翁炳孙
Owner SYNGEN BIOTECH
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