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Ganoderma tsugae-proteoglycan mixture and its preparing method and use

A technology of proteoglycan and pine fir and ganoderma lucidum, which is applied in the fields of peptide/protein components, drug combinations, and pharmaceutical formulations to achieve the effects of low viscosity, broad application prospects and high purity

Inactive Publication Date: 2007-03-07
CHANGCHUNG COLLEGE OF TRADITIONAL CHINESE MEDIICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on Ganoderma tsugae Murrill. is rarely reported, among which only Taiwan International Famous University declared the name "Polysaccharide-based extract from ganoderma, pharmaceutical use thereof, and process for preparing the same" in the United States in 2003. The patent discloses a process for obtaining the water-soluble crude extract of Pinus fir Ganoderma lucidum, but this process only involves steps such as water extraction, alcohol precipitation and deproteinization, and the obtained product is the primary crude product of Pinus fir Ganoderma lucidum

Method used

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  • Ganoderma tsugae-proteoglycan mixture and its preparing method and use
  • Ganoderma tsugae-proteoglycan mixture and its preparing method and use
  • Ganoderma tsugae-proteoglycan mixture and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, preparation pine fir Ganoderma lucidum protein-polysaccharide mixture

[0017] Weigh 100 grams of pine fir ganoderma fruiting body powder, add 1 liter of 80% ethanol, heat and reflux at 75-78 ° C for 2 hours, repeat 3 times, filter, add 2 liters of deionized water to the medicinal residue, heat to boil, stir at intervals, Extract for 3 hours each time, repeat 5 times, filter, combine extracts, concentrate to a volume of 0.5-1 liter, cool to room temperature, add 2.5 liters of absolute ethanol, stir while adding, place at 4°C for clarification, centrifuge (7000- 8000rpm, 15min), take the precipitate and dissolve it with water, 10μm and 1μm microfiltration, take the filtrate, then ultrafilter through 80KD, take the filtrate, and then use a 3KD membrane for ultrafiltration, the obtained retentate is concentrated and freeze-dried to obtain the pine fir ganoderma protein - Polysaccharide mixture.

Embodiment 2

[0018] Embodiment 2, preparation pine fir Ganoderma lucidum protein-polysaccharide mixture

[0019] Weigh 100 grams of pine fir ganoderma fruiting body powder, add 1.5 liters of 80% methanol, heat and reflux at 80°C for 3 hours, repeat 5 times, filter, add 4 liters of deionized water to the medicinal residue, heat to boil, stir at intervals, each time Extract for 3 hours, repeat 6 times, filter, combine the filtrates, concentrate to a volume of 1 liter, cool, add 4 liters of anhydrous methanol, stir while adding, place at 4°C for clarification, centrifuge (7000-8000rpm, 15min), and dissolve the precipitate into Water is subjected to 10 μm, 5 μm and 1 μm microfiltration, and the filtrate is taken through an 80KD ultrafiltration membrane, and the filtrate is taken, and then ultrafiltered with a 3KD membrane.

Embodiment 3

[0020] Example 3, Preparation of Pinus fir Ganoderma lucidum protein-polysaccharide mixture

[0021] Weigh 50kg of pine, fir and ganoderma fruiting body powder into an extraction tank, add 450 liters of 90% ethanol, under stirring, extract under reflux at 78°C for 2 hours each time, repeat 3 times, recover ethanol, add 1000 liters of water to the dregs, and heat To 100°C, extract for 3 hours, repeat 5 times, filter, combine the filtrates, concentrate to a volume of 250 liters, add 5 times the volume of absolute ethanol under stirring, place at a low temperature of 4°C, clarify, centrifuge (7000-8000rpm, 15 min), the precipitate was dissolved in deionized water, passed through 10 μm microfiltration, 1 μm microfiltration, and then 80KD ultrafiltration, concentrated with a 3KD membrane, and freeze-dried to obtain the Pinus fir ganoderma protein-polysaccharide mixture.

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Abstract

The present invention discloses Ganoderma tsugae proteoglycan mixture and its preparation process and application. The Ganoderma tsugae proteoglycan mixture with molecular weight of 6-12 KDa is prepared through the following steps: 1. degreasing crushed Ganoderma tsugae sporophore with alcohol; 2. extracting with deionized water and collecting extracted liquid; 3. precipitating extracted liquid with alcohol; 4. dissolving the precipitate with water and microfiltering; and 5. ultrafiltering 50-80 KDa ultrafiltered filtrate with 3-5 KDa membrane and DEAE-Cellulose chromatographic separating the membrane intercepted matter to obtain the refined Ganoderma tsugae proteoglycan mixture. The Ganoderma tsugae proteoglycan mixture of the present invention has obvious effects of inhibiting tumor and raising immunity and thus high medicinal value.

Description

technical field [0001] The present invention relates to a proteoglycan mixture and its preparation method and application, in particular to a proteoglycan mixture obtained from Ganoderma tsugae Murrill. and its extraction method and the proteoglycan mixture as injection or oral medicine Application of active ingredients. Background technique [0002] At present, there are three main ways to treat tumor diseases clinically: surgery, radiotherapy and chemotherapy. However, radiotherapy and chemotherapy seriously damage the normal cells and tissues of the human body, causing many patients to decline rapidly, and even cannot continue treatment. For a long time, people have been looking for new anticancer drugs with low toxicity and high tumor inhibition rate, and some results have been achieved. Since the occurrence and spread of tumors are closely related to the immune function of the human body, tumor immunotherapy has emerged. In the middle and late twentieth century, some ...

Claims

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Application Information

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IPC IPC(8): C07K1/34A61K38/16A61P35/00
Inventor 张洁姜大成费晓方
Owner CHANGCHUNG COLLEGE OF TRADITIONAL CHINESE MEDIICINE
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