Quality controlling method of ganoderma aqueous extract
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A detection method, water extract technology, applied in the field of detection and analysis of medicines or health care products, can solve the problem of high determination results of ganoderma lucidum polysaccharides
Active Publication Date: 2014-05-28
福建省食品药品质量检验研究院 +1
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Problems solved by technology
If it is determined according to the method of the Chinese Pharmacopoeia of the 2010 edition, the determination result of Ganoderma lucidum polysaccharide will be too high
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Embodiment 1
[0037] Embodiment 1 Determination of crude polysaccharide content
[0038] A1.1 Principle
[0039] The relative molecular mass in the sample is greater than 1×10 4 Precipitate high molecular substances in 80% ethanol solution, separate from monosaccharides and oligosaccharides in aqueous solution, use alkaline divalent copper reagent to selectively precipitate polysaccharides with dextran structure from other high molecular substances, and use phenol - Sulfuric acid reacts to generate an orange-red compound, and its content is determined by colorimetry, and its color intensity is proportional to the content of dextran in the crude polysaccharide, so as to calculate the content of the crude polysaccharide in this product.
[0044] A1.2.1.3 Sodium hydroxide solution (100 g / L): Weigh 25 g of sodium ...
Embodiment 2
[0074] Example 2 Ganoderma acid A, B, C 2 Identification and Quantitative Determination
[0075] A2.1 Principle
[0076] The sample was extracted with methanol as a solvent, ultrasonically extracted, and Ganoderma acid A and Ganoderma acid B were passed through a high-performance liquid chromatography column (C 8 ) separation; Ganoderma acid C 2 After high performance liquid chromatography column (C 18 ) separation, detected by ultraviolet detector (UV), respectively, according to the retention time of the chromatographic peak, and the external standard method of the peak area for quantification, respectively determine the ganoderma acid A, B and ganoderma acid C in the sample 2 content and calculate its total amount.
[0148] The index of symbolic components shall meet the requirements in Table 3.
[0149] Table 3 Indicators of iconic components
[0150]
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Abstract
The invention relates to a quality analysis analysis method of medicines or health care products, and specifically relates to a quality controlling method of a ganoderma aqueous extract. For controlling the product quality with high specificity, according to the invention, crude polysaccharide contents and a sum of ganoderma acid A, B, and C2 contents are adopted as quality controlling indexes of the ganoderma aqueous extract. According to the invention, ganoderma crude polysaccharide is extracted with a water extraction and alcohol precipitation method; polysaccharide with a glucan structure is precipitated by using a copper reagent; and the content of the polysaccharide is determined by using an ultraviolet spectrophotometry method. Therefore, the influences of auxiliary materials on determination are eliminated, and a basis is provided for quality controlling of the ganoderma aqueous extract. The contents of the characteristic components ganoderma acid A, B, and C2 in ganoderma lucidum, ganoderma sinnese, and ganoderma tsugae are relatively high. Compared to a currently commonly-used colorimetric method for determining total triterpenoid contents, the specificity of the method provided by the invention is high. With the method, purposes of authentication and quality control can be achieved.
Description
Technical field [0001] The invention involves a drug or health product detection and analysis method, which specifically involves a test method for Ganoderma lucidum water. Background technique [0002] Ganoderma lucidum is a perleral fungus Akota Chihi Ganoderma lucidum (Leyss.ex FR.) kars.Or Zizhi Ganoderma sinense Zhao, xu et zhang or Matsumi Ganoderma Ganoderma Tsugae Murr. The dry sub -entity of Tsugae Murr.Ganoderma extract is mainly water lifting and alcohol, and most of them use the water lifting process, that is, the porous bacteria family fungal Chizhi ( Ganoderma lucidum (Leyss. Ex fR.) kars.), Zizhi ( Ganoderma sinnese Zhao, xu et zhang) and Songshan Ganoderma ( Ganoderma Tsugae Murr. The drying sub -entities were made after the water extraction, concentration, and drying.Therefore, the present invention is named after Ganoderma lucidum water.Studies have shown that ganoderma contains a variety of chemical ingredients such as polysaccharides, trioma, nucleoside, alkal...
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