Artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr
A technology of artificial cultivation of pine and fir ganoderma, which is applied in the field of artificial cultivation of wild pine and fir ganoderma, can solve the problems that wild pine and fir ganoderma is not reported, and there is no wild pine and fir ganoderma, so as to improve immunity, meet a wide range of needs, and apply wide range of effects
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Embodiment 1
[0028] Embodiment 1: Use the present invention to carry out artificial cultivation of wild pine fir ganoderma
[0029] 1) Boil the green pine sawdust grown by wild pine, fir and ganoderma lucidum for 10 minutes, add wort juice and agar according to the ratio of 15% wort, 2% agar, and 10% boiled liquid of green gang sawdust, and pack into test tubes, 0.11MPa atmospheric pressure , 121 ℃ high temperature and high pressure damp heat sterilization for 30 minutes;
[0030] 2) According to the mass percentage, the raw material composition is 20% of potato, 2% of glucose, 2% of agar, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate and trace vitamin B1. The comprehensive PDA slope is conventionally produced, with 0.11MPa atmospheric pressure and 121℃ high temperature High pressure damp heat sterilization for 30min;
[0031] 3) Calculated by mass percentage, it is 78% sorghum, 20% millet, and CaCO 3 2% raw material composition, water content 60%, make the original ...
Embodiment 2
[0039] Embodiment 2: use the present invention to carry out the artificial cultivation of wild pine fir ganoderma
[0040] 1) Boil the green pine sawdust grown by wild pine, fir and Ganoderma lucidum for 12 minutes, add wort juice and agar according to the ratio of 15% wort, 2% agar, and 10% boiled liquid of green gang wood chips, and pack into test tubes, 0.11MPa atmospheric pressure , 121 ℃ high temperature and high pressure damp heat sterilization for 30 minutes;
[0041] 2) According to the mass percentage, the raw material composition is 20% of potato, 2% of glucose, 2% of agar, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate and trace vitamin B1. The comprehensive PDA slope is conventionally produced, with 0.11MPa atmospheric pressure and 121℃ high temperature High pressure damp heat sterilization for 30min;
[0042] 3) Calculated by mass percentage, it is 75% sorghum, 23% millet, and CaCO 3 2% and 55% water content to make the original seed material...
Embodiment 3
[0050] Embodiment 3: use the present invention to carry out the artificial cultivation of wild pine fir ganoderma
[0051] 1) Boil the green pine sawdust grown by wild pine, fir and Ganoderma lucidum for 8 minutes, add wort juice and agar according to the ratio of 15% wort, 2% agar, and 10% boiled liquid of green gang sawdust, and divide into test tubes, 0.11MPa atmospheric pressure , 121 ℃ high temperature and high pressure damp heat sterilization for 30 minutes;
[0052] 2) According to the mass percentage, the raw material composition is 20% of potato, 2% of glucose, 2% of agar, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate and trace vitamin B1. The comprehensive PDA slope is conventionally produced, with 0.11MPa atmospheric pressure and 121℃ high temperature High pressure damp heat sterilization for 30min;
[0053] 3) Calculated by mass percentage, it is 79% sorghum, 20% millet, and CaCO 3 1% and 58% water content to make the original seed material, ...
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