Method for obtaining rare ginsenoside by fermentation of Ganoderma tsugae liquid

A liquid fermentation and ginsenoside technology, applied in the field of biomedicine, can solve the problems of high cost, complicated operation, and high substrate requirements, and achieve the effect of easy operation, simple fermentation process, and less stringent environmental requirements

Inactive Publication Date: 2016-09-07
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first object of the present invention is to provide a method for obtaining rare ginsenosides by liquid fermentation of Pinus fir ganoderma lucidum, so as to solve the problems of high substrate requirements, high cost and complicated operation in the existing rare ginsenoside acquisition process; The method of obtaining rare ginsenosides by liquid fermentation of pine, fir and ganoderma lucidum combines medicinal bacteria and traditional Chinese medicine through fermentation to obtain fermentation bacteria; it has the advantages of simple fermentation process, low environmental requirements, easy operation, etc., which is beneficial to industrial applications and industrial development

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  • Method for obtaining rare ginsenoside by fermentation of Ganoderma tsugae liquid

Examples

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Embodiment 1

[0050] The method for obtaining rare ginsenosides by liquid fermentation of Pinus fir ganoderma lucidum provided in this example comprises the following steps:

[0051] The parent species of Pinus fir Ganoderma lucidum is made into a secondary liquid strain, which is inserted into the water extract fermentation substrate of Araliaceae Panax genus for fermentation to obtain the fungal substance containing rare ginsenosides;

[0052] Wherein, the volume ratio of the liquid strain to the liquid fermentation substrate is 1:22.

Embodiment 2

[0054] The method for obtaining rare ginsenosides by liquid fermentation of Pinus fir ganoderma lucidum provided in this example comprises the following steps:

[0055] Put the mother species of Pinus fir ganoderma into the PDB medium, and cultivate it on a shaking table at 22°C and 200 rpm for 6 days in the dark to obtain a first-class liquid strain;

[0056] Put the first-level liquid strains into the second-level strain culture medium, and cultivate them in a shaking table at 22°C and 200 rpm for 6 days in the dark to obtain the second-level liquid strains;

[0057] The second-level liquid bacteria were inserted into the fermentation medium of American ginseng water extract, cultured on a shaker, and cultured in the dark at 200 rpm for 13 days to obtain bacteria containing rare ginsenosides;

[0058] Wherein, the volume ratio of the liquid strain to the liquid fermentation substrate is 1:18.

[0059] The preparation method of the above-mentioned American ginseng water extr...

Embodiment 3

[0062] The method for obtaining rare ginsenosides by liquid fermentation of Pinus fir ganoderma lucidum provided in this example comprises the following steps:

[0063] Select the fruiting bodies of wild pine and fir Ganoderma lucidum from dead tree trunks in the forests of Changbai Mountains as the mother species of pine and fir ganoderma, put the mother species of pine and fir ganoderma into PDB medium, and cultivate them in a shaker at 24°C and 220 rpm. Under the dark conditions, cultivated in the dark for 8 days, and obtained the first-class liquid strain;

[0064] Inoculate the first-level liquid strains into the second-level liquid strain culture medium, and culture them in the dark for 6 days at 24°C and 210 rpm to obtain the second-level liquid strains

[0065] The secondary liquid strains were inserted into the ginseng water extract fermentation medium, cultured on a shaking table, and cultured in the dark at 210 rpm for 15 days to obtain bacteria containing rare gins...

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Abstract

The invention provides a method for obtaining rare ginsenoside by the fermentation of Ganoderma tsugae liquid. The method comprises the following steps: manufacturing a Ganoderma tsugae mother strain into a liquid strain, inoculating ginseng or American ginseng into a water extract fermentation matrix for fermentation to obtain mycoplasma which contains the rare ginsenoside, wherein the volume ratio of the liquid strain to the liquid fermentation matrix is 1:(18-22). The invention provides the method for obtaining the rare ginsenoside by the fermentation of the Ganoderma tsugae liquid. Medicinal fungi and traditional Chinese medicines are combined through fermentation to obtain the fermentation mycoplasma. The method has the advantages of simple fermentation technology, easiness in operation and the like, is not harsh to environment requirements, and is favorable for industrial application and industry development. The product mycoplasma contains the rare ginsenoside Rg3, Rk1, Rg5 and C-K, meanwhile, a new thought and a new method are provided for the development and the utilization of the Ganoderma tsugae.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for obtaining rare ginsenosides by liquid fermentation of pine, fir and ganoderma lucidum. Background technique [0002] Ginseng (Panax ginseng) is the dried root of Araliaceae plant ginseng. As a precious medicinal material, it has the effects of invigorating vital energy, rejuvenating veins, strengthening spleen and lungs. American ginseng (scientific name: Panax quinquefolius) is a perennial herb of the Araliaceae genus Panax, also known as American ginseng, American ginseng, and American ginseng. It can nourish qi and nourish yin, clear heat and promote body fluid; it can be used for qi deficiency and yin deficiency, internal heat, cough, asthma, phlegm and blood, deficiency heat, tiredness, thirst, dry mouth and throat. [0003] Ginsenoside is a glycoside compound formed by linking sugar and aglycon. It belongs to triterpenoid saponin and is one of the main act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/645
CPCC12P33/20
Inventor 闫梅霞张瑞韩红祥王佳王志清王英平逄世峰许世泉
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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