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Ectomycorrhizal helper bacteria bacillus pumilus and application thereof

A technology of Bacillus pumilus and ectomycorrhizal, applied in applications, bacteria, biocides, etc.

Active Publication Date: 2010-12-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the interaction between MHB and mycorrhizal fungi in foreign countries has a history of several decades, and a large number of MHBs have been screened out, and some excellent MHBs have been studied in depth. See screening studies on MHB and related reports on the interaction between MHB and mycorrhizal fungi

Method used

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  • Ectomycorrhizal helper bacteria bacillus pumilus and application thereof
  • Ectomycorrhizal helper bacteria bacillus pumilus and application thereof
  • Ectomycorrhizal helper bacteria bacillus pumilus and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the growth-promoting effect of bacillus pumilus HR10 to sclerotidia flavum (R1)

[0025] After activating the bacteria to be tested, use an inoculation loop to inoculate a small amount into a 100mL Erlenmeyer flask containing 50mL TSA liquid medium, and incubate at 28°C with shaking at 160r / min for 72h. Pour the bacterial suspension into a sterilized centrifuge tube, centrifuge at 5000r / min at 4°C for 5min, pour off the supernatant, then wash the bacteria twice with normal saline, and finally add physiological Saline, after mixing, it will be the bacterial cell suspension of HR10 strain (about 5×108cfu / mL). At the same time, draw the supernatant with a sterile syringe, and filter it with a bacterial filter (the filter membrane has a pore size of 0.22 μm and a diameter of 0.25 mm), which is the sterile filtrate of the HR10 strain.

[0026] (1) Determination of the growth-promoting effect of Bacillus pumilus HR10 cell suspension on Plasmodium flavus (R1)

...

Embodiment 2

[0039] Example 2: The growth-promoting effect of the interaction between Bacillus pumilus HR10 and M. flavus (R1) on the growth of black pine.

[0040] Cultivation of sprouts: Black pine seeds are soaked in warm water at 60°C for 24 hours to accelerate germination, soaked in 0.5% potassium permanganate solution, sterilized for 2 hours, rinsed with tap water for 1 hour, sown in sterilized sand, and placed in 25 Cultivate in a greenhouse at ℃, and cut the roots and transplant the seedlings when they grow to the cotyledon stage.

[0041] Potted substrate: The substrate soil was collected from the back hill of Nanjing Forestry University campus, and the soil sample was sieved by 1.01×10 6 Pa sterilization, 90min, cool down and set aside.

[0042] Preparation of Bacillus pumilus HR10 bacterial cell suspension: After activating the test bacteria, use an inoculation loop to inoculate a small amount into a 100mL Erlenmeyer flask containing 50mL TSA liquid medium, culture at 28°C and ...

Embodiment 3

[0049] Embodiment 3: bacillus pumilus HR10 is to rhizoctonia (Rhizoctonia sp.) plate bacteriostasis

[0050] Inoculate the Bacillus pumilus HR10 strain activated on the NA slant by streaking on both sides of the PDA plate, and then use a puncher with a diameter of 5mm to punch out the same quality and amount of bacteria blocks from the PDA plate culture of the pine seedling damping-off disease pathogen, and then point Connected to the middle of the plate. Incubate at 28°C for 7 days to observe the presence or absence of the inhibition zone, and record its width. From Table 5 and Figure 4 It can be seen that HR10 has an antagonistic effect on the pathogenic mycobacteria Rhizoctonia sp. Compared with the control, the bacteriostatic rate reached 85.58%.

[0051] Table 5 Inhibition of Bacillus pumilus HR10 to Rhizoctonia sp.

[0052]

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Abstract

The invention discloses ectomycorrhizal helper bacteria bacillus pumilus HR10. The classified nomenclature of the ectomycorrhizal helper bacteria bacillus pumilus HR10 is bacillus pumilus, the bacillus pumilus is preserved in CCTCC, the collection number of the bacillus pumilus is CCTCC No: M 2010143 and the preservation date is June 12th, 2010. The bacillus pumilus HR10 is plant growth-promoting rhizobacteria, has direct promoting effect on black tree growth and can promote the growth of rhizipogen luteous of ectomycorrhizal fungi; double inoculation of the bacillus pumilus HR10 and the rhizipogen luteous has obvious growth-promoting effect on pinus thunbergii parl; the bacillus pumilus HR10 has inhibiting effect on pathogenic rhizoctonia of pine nursery stock damping-off disease; and greenhouse preventing and controlling experiments show that the bacillus pumilus HR10 strains can effectively prevent and control the pine nursery stock damping-off disease.

Description

technical field [0001] The invention belongs to the field of microbial fertilizers, and in particular relates to an ectomycorrhizal auxiliary bacterium Bacillus pumilus and its application in promoting the growth of black pine and preventing and controlling pine seedling damping-off. Background technique [0002] Plant growth-promoting rhizobacteria (PGPR) is a general term for bacteria in the rhizosphere microflora that can promote plant growth, prevent disease, and increase crop yield. Since Burr et al. first reported PGPR on potatoes in 1978, more than 20 species of PGPR have been discovered at home and abroad. Among them, Pseudomonas, Bacillus, Agrobacterium, Eriwinia, Flavobacterium, Pasteuria , Serratia (Serratia), Enterobacter (Enterobacter) and so on are the most prominent. Plant rhizosphere growth-promoting bacteria and ectomycorrhizal fungi (Ectomycorrhizal Fungi, EMF), as an important group of plant rhizosphere microorganisms, play an important role in plant nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/04A01P3/00A01P21/00C05F11/08C12R1/07
Inventor 吴小芹盛江梅侯亮亮刘辉叶建仁
Owner NANJING FORESTRY UNIV
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