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Rapid seedling culture method for blueberries and special culture vessel

A blueberry, fast technology, applied in the field of agriculture, can solve the problems of incapable of factory application, ineffective fixation, easy pollution of explants, etc., and achieve the effect of increasing the speed of seedling cultivation, not being easily injured, and reducing costs

Inactive Publication Date: 2019-07-02
天津显硕科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these agar substitutes have one or more of the problems of easy contamination of explants, difficult inoculation, easy injury, ineffective fixation, and incapable of factory application.

Method used

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  • Rapid seedling culture method for blueberries and special culture vessel
  • Rapid seedling culture method for blueberries and special culture vessel
  • Rapid seedling culture method for blueberries and special culture vessel

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Experimental program
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Embodiment

[0026] Embodiment: In order to achieve the above object, the technical solution adopted in the present invention comprises the following steps:

[0027] (1), preparation solution: prepare 4 kinds of test mother solutions of macroelement mother solution, trace element mother solution, organic substance mother solution and iron salt mother solution of B5 medium, prepare 1mg / ml IBA, 1mg / ml IAA, 1mg / ml NAA3 kinds of hormone solutions;

[0028] (2), preparation medium: Weigh 30g of sucrose, put it into a 1000ml container, add 500ml of cold distilled water, heat and stir to dissolve the sucrose, absorb 50ml of macroelement mother liquor, 5ml of trace element mother liquor, 5ml of organic matter mother liquor and 5ml of iron salt mother liquor Add to the above container, then add 0.5ml IBA, 0.3ml IAA, 0.1ml NAA three kinds of hormone solutions, stir well, add distilled water to 1000ml, adjust the pH to 5.4, and pack the culture medium into the outer box of the tip box while it is hot ...

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Abstract

The invention discloses a rapid seedling culture method for blueberries and a special culture vessel. The rooting method comprises the following steps that a solution is prepared, specifically, a liquid culture medium containing a certain type and concentration of hormones is prepared, the culture medium is separately packaged in outer box bodies of sucker boxes, supports are placed, the outer boxbodies are wrapped with edible fungus bags, and then high-pressure steam sterilization, inoculation and rooting culture are carried out. The special culture vessel is formed by modifying a sucker boxand comprises the sucker box and a support arranged in the sucker box, the support is provided with a plurality of support holes, and the height, the number of the holes and the aperture of the support are matched with the length and the thickness of propagated shoots. The rapid seedling culture method and the special culture vessel have the beneficial effects that the formula of the culture medium is optimized, the support is placed in the sucker box, so that the test-tube rooting rate of tissue culture seedlings of the blueberries is greatly increased, the root system quality indexes such as the rooting amount and the root length are also improved, and therefore the problem that a liquid rooting technology lacks an effective fixing support can be solved, and the inhibition of a solid culture medium agar coagulator on the tissue culture seedlings can be avoided.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a rapid seedling raising method for blueberries and a special culture vessel. Background technique [0002] Blueberry, scientific name lingonberry, is a perennial deciduous fruit tree in the form of a shrub. The blueberry fruit is a blue berry, nearly round, with delicate flesh, very small seeds, moderately sweet and sour, and has a refreshing and pleasant aroma. Blueberry fruit is very nutritious and rich in superoxide dismutase (SOD), which is a scavenger for eliminating excess oxygen free radicals in the human body. It not only has good nutritional and health effects, but also prevents brain aging, strengthens the heart, fights cancer, Soften blood vessels, enhance the body's functional immunity and other functions. Blueberry not only has high nutritional value, but also has high economic value. It is the fastest growing third-generation fruit tree variety in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨代显
Owner 天津显硕科技有限公司
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