A kind of liquid rooting method and special culture container of blueberry tissue culture seedling

Abstract

The invention discloses a liquid rooting method of blueberry tissue culture seedlings and a special culture container. The rooting method comprises the following steps: preparing a solution, preparing a liquid medium containing a certain type and concentration of hormones, and packing the medium into a tip box and an outer box In the middle, place the bracket, wrap the edible mushroom bag and then sterilize under high pressure steam, inoculate, and root culture; the special culture container is transformed from the tip box, including: the tip box and the bracket set in the tip box, and the bracket is formed with a Several support holes, the height, number of holes, and aperture of the support are compatible with the length and thickness of the proliferating shoots. The invention is beneficial in that: by optimizing the formula of the medium and placing a bracket in the tip box, the rooting rate of blueberry tissue culture seedlings in the bottle is greatly improved, and the root system quality indicators such as the number of roots and root length of the proliferating new shoots are also improved. , not only solves the problem that the liquid rooting technology lacks an effective fixed support, but also avoids the inhibition of the solid medium agar coagulant on the tissue culture seedlings.

Description

technical field [0001] The invention relates to a method for promoting rooting of plants, in particular to a method for promoting rooting of blueberry tissue culture seedlings by using a liquid medium, and belongs to the technical field of plant cultivation. Background technique [0002] Blueberry (blueberry), also known as lingonberry, is a perennial shrub of the Rhododendronaceae Vaccinium genus. It is a new tree species with high economic value and broad development prospects. The fruit is blue, the size of the fruit varies with the variety, and the weight of a single fruit is 0.5-2.5 grams. The pulp is delicate, the seeds are small, sweet and sour, and the flavor is unique. It is one of the representative varieties of the fastest growing third-generation fruit trees in the world today. At the same time, blueberry fruit is rich in nutrition and can be eaten fresh directly, and has strong medicinal value and nutritional and health functions. [0003] Plant tissue culture...

AI Technical Summary

Problems solved by technology

However, these agar substitutes have one or more of the problems of easy contamination of explants, difficult inoculation, easy injury, ineffective fixation, and incapable of factory application.

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