31results about How to "Excellent breed" patented technology

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h/d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

The invention relates to a tissue culture method for cultivating amaryllis vittata by utilizing bulbs. The tissue culture method comprises the following steps: selecting and disinfecting explants; performing induction culture; performing subculture multiplication culture; rooting; transplanting. According to the invention, small bulbs of amaryllis vittata are taken as explants, so that the reduction of contamination rate is benefited and the induction rate is increased; cluster buds can be induced without callus stage; the cluster buds can be directly induced so as to acquire bulbs; the induction time is shortened; the buds can be added for multiplication culture; the multiplied test bulbs are rooted; one-step bulb forming of amaryllis vittata bulbs can be realized according to the invention; the time of tissue culture is saved; the operation steps are reduced; the excellent natures of original bulbs are kept; an effective method is supplied for industrial efficient cultivation of amaryllis vittata; a technical support is supplied for the industrial development of amaryllis vittata.

The invention discloses a method utilizing herbicide Barban to rapidly cultivate asparagus all male plants strain, which comprises the following steps: 1) amphoteric strain is chosen from the asparagus varieties for production purpose; the amphoteric strain produces asparagus seeds by homologous strain inbred; 2) the asparagus seeds are soaked in Barban, and bud forcing treatment is carried out to the asparagus seeds; 3) the processed asparagus seeds are cultivated in a nutrition pot; flowering takes place after 30 to 35 days; each individual plant respectively hybrid with a female strain, and the seeds of each individual plant are reserved; 4) the seed reserved from each individual plant are processed again by Barban; after flowing, the ratio of male and female flowering is calculated, and super male plants are identified; 5) super male plant clones are reserved and propagated by in vitro culture method; 6) the super male plant is used as the male parent to hybrid excellent female line, and asparagus all male plants strain can be acquired. The method has the advantages that, the time of asparagus from seedling to flowering is greatly reduced; the identification process of super male plants is accelerated; the invention has an important role in the breeding of asparagus all male plants strain, and the application prospect is wide.

The invention discloses an angelica tissue culturing and breeding method, which comprises the following steps: adopting angelica root as external growing bulk, sterilizing, grafting to induce callus, breeding, proceeding bud and root induction, forming entire plant sprout, planting the entire sprout in the substrate, adopting MS culture medium as base and 6-furfuryl adenine, 6-benzyl adenine, 2, 4-dichlorophenoxyacetic acid, fruitone, sucrose and active carbon as auxiliary compositions.

The invention provides a method for quickly breeding plumeria rubra by tissue culture. The method comprises the following steps of carrying out tissue culture and rapid propagation with MS (Murashige and Skoog) as a minimal medium and plumeria rubra leaves as explants under different hormone components and concentration levels; carrying out sterilization treatment and inoculation induction to form a callus tissue, and then carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; and finally carrying out acclimatization and transplantation, wherein the minimal medium is based on the MS culture medium, and supplemented with components including 6-benzyl aminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the invention, tissue culture is applied to overcome the problem that the plumeria rubra is too long in seedling-raising period and low in propagation coefficient, so that factory-like quick seedling raising can be carried out for meeting the demands of the market on the plumeria rubra.

The invention discloses a method for prolonging the life of ratoons by filling healthy sugarcane seedling bud recesses with seeds. The method comprises the following steps: cutting the sugarcane healthy seed bud recesses from a sugarcane stem, disinfecting and sterilizing the sugarcane healthy seed bud recesses, culturing the sugarcane healthy seed bud recesses in a seedling culture tray till 4 to5 main leaves grow, and then applying the obtained seedlings to fill newly planted and ratoon sugarcanes in conventional planting to prolong the life of the ratoons. According to the method, sugarcane seeds are obtained, while the remaining sugarcane stem is still an overall sugarcane stem and can be continuously delivered into a sugar refinery for sugar refining, so that the sugarcane stems aregreatly saved; after the healthy sugarcane seedling bud recesses are cut off, the bud recesses are germinated and grow rapidly through nutritional seedling culture at first, and then seed-lacking places of the newly planted and ratoon sugarcanes in conventional planting are filled with the seeds, so that the seed-filled sugarcane seedlings and the previous sugarcane seedlings grow consistently, and the life of the ratoons is prolonged.

The invention provides a tissue culture propagation method of Gynura formosana. The method comprises the following steps of: with the stem of Gynura formosana as an explant, carrying out sterilizing, grafting and inducing operations to form callus; then, forming an integral plant seedling through proliferation of callus, bud induction and root induction; transplanting the integral plant seedling to a culture medium so that the integral plant seedling grows up inti normal Gynura formosana plant, wherein the basic culture medium is based on MS culture medium and assisted with components such as L-proline, 6-benzyladenine, 2, 4-dichlorphenoxyacetic acid, naphthylacetic acid, cane sugar, active carbon and the like. The method provided by the invention overcomes the problem of long culturing period and low propagation coefficient of Gynura formosana by using tissue culture, and industrial quick seedling can be performed, so that the method meets demand on Gynura formosana in market.

The invention discloses a phoebe bournei grafting and seedling raising method. Strong one-year phoebe bournei seedlings with the height of 40-60 cm and the ground diameter of 5-7 mm are selected as rootstock, in-season sprouting branches are selected from good provenance select trees with the height 15% larger than average height and the diameter at breast height 50% larger than average diameter at breast height or more to serve as scions, after grafting, the rootstock and the scions are prevented from being bruised or tilted and broken, sprouts are removed, bound mulching films are cut open in time, fertilizer and water management, weeding and disease and pest prevention are conducted well in time during the period, and good strong phoebe bournei grafting seedlings are obtained. The seedling raising method is easy and quick to operate, the grafting survival rate is high, new seed collecting mother plant dwarf can be promoted, the flowering and fruiting stages are earlier, safety of manual seed and cutting collection is improved, production cost is reduced, fecundity of good phoebe bournei seedlings is improved, good seed supply capacity is improved, and the requirements of development of the phoebe bournei industry are met.

The invention discloses a quick red bean local variety purifying and rejuvenating method and relates to the technical field of crop variety resource protection and utilization. The method comprises the steps: performing large group selection to select single plants and performing plant laboratory test and forming plant rows; identifying and comparing multiple plant rows and forming plant lines through the plant laboratory test; comparing multiple plant lines to predict yield and selecting high-yield plant systems through the plant laboratory test; performing mixed line breeding and producing qualified seeds. The method disclosed by the invention widens selected groups, can perform plant laboratory test according to red bean representations, ensures population genetic stability, reduces gene loss generation, quickly increases population quantity and increases output quantity of breeder seeds; seed propagation generations are reduced, a mixing opportunity is reduced, and a natural selection effect is reduced; meanwhile, a red bean local variety purifying and rejuvenating process is quickened, excellent variety characteristics are kept, germplasm resources with local characteristics are effectively protected, and the breeder seeds can be directly applied to large field production or the breeder seeds can be utilized to directly produce seeds.

The invention relates to a quality walnut tissue culture quick propagation method which comprises, appending right amount of iron ions and zinc ions into chestnut culture medium, adjusting nutrient content of culture medium, screening the most suitable culture medium for chestnut growth, selecting right explant for tissue culture at right stage, readjusting the culture medium formulation so as to grow shoot and root for the chestnut, transplanting it into warm house and planting into big nourishing cup equipped with nourishment base materials, finally planting and transplanting the nursery stock in the nourishment cup.

The invention relates to a tissue culture method of ornamental-aquatic-plant Hydrotriche hottoniflora. The method includes the steps of firstly, preprocessing; secondly, cutting preprocessed explantsinto explants 1-1.5cm in length, wherein the each cut explant at least comprises one axillary bud; inoculating the cut explants into a primary culture media to perform primary culture; thirdly, separating primary adventitious buds in the primary culture media to control the length of the primary adventitious buds to be within 2cm; transferring the separated primary adventitious buds into proliferation culture media to perform culture; fourthly, transferring plants with the length being larger than 3-4cm in the proliferation culture media into rooting culture media to perform culture; fifthly,taking the Hydrotriche hottoniflora plants with complete root systems out of the rooting culture media, washing with clean water, and transplanting into an ecological tank. The method has the advantages that the method is unaffected by seasons, temperature and regions and can acquire a large number of new plants, the sterile reproduction system of Hydrotriche hottoniflora can be built fast, technical support is provided for the keeping of the excellent variety characters of the Hydrotriche hottoniflora, and sterile seedlings can be constantly provided for vast Hydrotriche hottoniflora lovers.

The invention relates to an asexual propagation method of proserpinaca palustris. The method includes: (1), pre-treating an explant; (2), cutting the explant after being pre-treated into a plurality of small explants, and inoculating the small explants onto an induced culture medium for culture until clustered shoots are formed, wherein each small explant has 1-3 stem nodes; (3), separating the clustered shoots in the induced culture medium to control length of the clustered shoots to be 2-3cm; inoculating the clustered shoots after being separated into a proliferation culture medium for culture, separating new shoots when growing to 2-3cm, inserting the new shoots into the proliferation culture medium, and repeating subculture for 3-4 times to obtain clustered shoots after large-scale propagation; (4), splitting down the clustered shoots of 3-5cm in length in the clustered shoots after propagation from bases to shoot tubers, cutting, and planting. By the method, a lot of new plants can be obtained without being subject to season, temperature and regional impact, so that a proserpinaca palustris sterile propagation system can be established quickly, technical support can be provided for maintaining excellent seed characteristics of proserpinaca palustris, and ceaseless sterile seedlings are provided for the majority of fans.

The invention discloses a tissue culture method of wild sambucus williamsii and belongs to the field of plant tissue culture. An asexual reproduction method of the sambucus williamsii is in a blank stage. The tissue culture method of wild sambucus williamsii comprises the following steps: selecting a stem segment with a lateral bud as an explant; conducting disinfection and inoculating the disinfected explant into a primary culture medium for culturing to form the explant with a bud; inoculating the obtained explant with a bud into a proliferation culture medium for culturing; cutting off an adventitious bud and inoculating the adventitious bud into an MS culture medium for culturing; and and inoculating the cultured adventitious bud into a rooting culture medium for culturing. The method has advantages of having a high propagation coefficient, a short propagation period, and low cost and keeping good varieties.