31results about How to "Excellent breed" patented technology

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for tissue culture and rapid propagation of cherry plums. The specific steps of the method are as follows: sterilize and sterilize the stem section of the axillary buds of the cherry plum as explants, first inoculate them on the bud induction medium for primary culture to obtain the axillary buds, and then inoculate the axillary buds on the proliferation medium for propagation and culture , the multi-cluster buds obtained from multiplication were cut and divided into 2-4 small parts and inoculated into GA-containing 3 Heightening culture was carried out on the heightening medium, and then the sterile seedlings with a height of more than 2 cm were excised and inoculated on the rooting medium to take root, and the remaining low and dense multi-cluster buds were subcultured on the proliferation medium and cycled Wait until the aseptic seedling base on the rooting medium grows white taproots and some fibrous roots, then harden the seedlings, transplant them into the substrate, and obtain seedlings of cherry plums. The tissue culture rapid propagation method of the present invention is easier to obtain strong seedlings than single-use multiplication culture, and the multiplication coefficient is more than 3 times higher than that of single-use increased culture.

The invention discloses a sugarcane healthy seedling bud eye seedling and a mechanical transplanting method thereof. The method comprises the following steps of cutting the sugarcane healthy seedlingbub eye from a sugarcane stem, sterilizing, cultivating the bub eye in a seedling tray to grow to 4-5 pieces of true leaves, and then transplanting into fields by machinery. The method can obtain sugarcane seeds while the sugarcane stem is still a whole sugarcane stem, can continue to enter a sugar house for sugar extraction, and greatly save the sugarcane stem; the sugarcane healthy seedling budeye are cut and then subjected to nutrient seedling cultivation and then transplanted by the machinery, sugarcane bud rate and transplant survival rate are high, and the method can quickly get sugarcane seedlings, be suitable for large-scale planting of sugarcane, greatly reduce the cost of sugarcane cultivation and improve the planting efficiency of the sugarcane.

The present invention relates to a kind of tissue culture method of ornamental aquatic plant Antarctica, comprising the following steps: (1) pretreatment; (2) cutting the pretreated explant into 1-1.5cm long explant, each cut Each explant has at least one axillary bud; the explant after cutting is inserted into the first-generation culture medium and carried out the first-generation culture; (3) separate the first-generation adventitious buds in the first-generation medium, so that the length of the first-generation adventitious buds is controlled within 2cm; The first-generation adventitious buds after that are transferred to the propagation medium for cultivation; (4) the plants with a length greater than 3-4cm in the propagation medium are transferred to the rooting medium for cultivation; Take it out from the base, rinse it with clean water and transplant it into an ecological tank. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, and quickly establish an aseptic propagation system for Aratarctica. Seedlings.