Tissue culture method of ornamental-aquatic-plant Hydrotriche hottoniflora

Abstract

The invention relates to a tissue culture method of ornamental-aquatic-plant Hydrotriche hottoniflora. The method includes the steps of firstly, preprocessing; secondly, cutting preprocessed explantsinto explants 1-1.5cm in length, wherein the each cut explant at least comprises one axillary bud; inoculating the cut explants into a primary culture media to perform primary culture; thirdly, separating primary adventitious buds in the primary culture media to control the length of the primary adventitious buds to be within 2cm; transferring the separated primary adventitious buds into proliferation culture media to perform culture; fourthly, transferring plants with the length being larger than 3-4cm in the proliferation culture media into rooting culture media to perform culture; fifthly,taking the Hydrotriche hottoniflora plants with complete root systems out of the rooting culture media, washing with clean water, and transplanting into an ecological tank. The method has the advantages that the method is unaffected by seasons, temperature and regions and can acquire a large number of new plants, the sterile reproduction system of Hydrotriche hottoniflora can be built fast, technical support is provided for the keeping of the excellent variety characters of the Hydrotriche hottoniflora, and sterile seedlings can be constantly provided for vast Hydrotriche hottoniflora lovers.

Description

technical field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of ornamental aquatic plant Antarctica. Background technique

[0002] Antarctica is a commonly used ornamental aquatic plant. Antarctica is a submerged herb, plantaceae, dicotyledons, emergent aquatic plants. In the wild, every flowering period, the water surface is as beautiful as a sea of ​​flowers, and you can also see it blooming in a plant tank. This kind of aquatic plants can adapt to a wide range of water quality changes in the water, and can also grow under low light, but it is still better to cultivate under stronger light. Antarctica is more like a green feather in shape, the difference is that the former has needle-like leaves and the latter has pinnate leaves. The current propagation method of Antarctica fir is cutting and lateral bud propagation.

[0003] However, there is still a lack of mature technology for large-scale ...

Examples

Embodiment 1

[0026] Step 1: Prepare primary culture medium, proliferation medium and rooting medium

[0027] 1. The first-generation culture medium, proliferation medium and rooting medium used in this embodiment all adopt MS medium as the basic medium, and the formula of MS medium is as follows: mg / L

[0028] Table 1 MS medium formula

[0029] serial number components Content (mg / L) serial number components Content (mg / L) 1 KNO 3 :

1900 11 CuSO 4 .5H 2 o

0.025 2 NH 4 NO 3

1650 12 CoCL 2 .6H 2 o

0.025 3 MgSO 4 ·7H 2 o

370 13 Na 2 -EDTA

37.3 4 K H 2 PO 4

170 14 FeSO 4 ·7H 2 o

27.8 5 CaCl 2 2H 2 o

440 15 Glycine 2.0 6 MnSO 4 4H 2 o

22.3 16 Pyridoxine Hydrochloride 0.5 7 ZnSO4·7H 2 o

8.6 17 Ammonium sulfate hydrochloride 0.1 8 h 3 BO 3

6.2 18 niacin 0.5 9 KI 0.83 19 Creatine 100 10 Na 2 MoO 4 ·7H 2 o

...

Embodiment 2

[0048] Step 1: Prepare primary medium, proliferation medium and rooting medium

[0049] 1. The first generation, proliferation and rooting medium prepared in the present embodiment are the MS medium that is adopted as the base medium, and the formulation of the MS medium is as shown in Example 1.

[0050] 2. Preparation of primary culture medium

[0051] Add 6g of agar, 20g of sucrose, 1.0mg of kinetin (KT), and 0.05mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; aliquot Put it into the tissue culture bottle, and the height of the culture medium is 1-1.5cm, which is the best.

[0052] 3. Proliferation medium preparation

[0053] Add 6g of agar, 20g of sucrose, 2.5mg of 6-aminopurine (6BA), and 0.75mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Put...

Embodiment 3

[0067] Step 1: Prepare primary medium, proliferation medium and rooting medium

[0068] 1. The first generation, proliferation and rooting medium prepared in the present embodiment are the MS medium that is adopted as the base medium, and the formulation of the MS medium is as shown in Example 1.

[0069] 2. Preparation of primary culture medium

[0070] Add 10g of agar, 10g of sucrose, 1.5mg of kinetin (KT), and 0.5mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20 minutes before use; aliquot Put it into the tissue culture bottle, and the height of the culture medium is 1-1.5cm, which is the best.

[0071] 3. Proliferation medium preparation

[0072] Add 10g of agar, 10g of sucrose, 1.75mg of 6-aminopurine (6BA), and 1.0mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Put it ...

technical field

[0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of ornamental aquatic plant Antarctica. Background technique

[0002] Antarctica is a commonly used ornamental aquatic plant. Antarctica is a submerged herb, plantaceae, dicotyledons, emergent aquatic plants. In the wild, every flowering period, the water surface is as beautiful as a sea of ​​flowers, and you can also see it blooming in a plant tank. This kind of aquatic plants can adapt to a wide range of water quality changes in the water, and can also grow under low light, but it is still better to cultivate under stronger light. Antarctica is more like a green feather in shape, the difference is that the former has needle-like leaves and the latter has pinnate leaves. The current propagation method of Antarctica fir is cutting and lateral bud propagation.

[0003] However, there is still a lack of mature technology for large-scale ...

Examples

Embodiment 1

[0026] Step 1: Prepare primary culture medium, proliferation medium and rooting medium

[0027] 1. The first-generation culture medium, proliferation medium and rooting medium used in this embodiment all adopt MS medium as the basic medium, and the formula of MS medium is as follows: mg / L

[0028] Table 1 MS medium formula

[0029] serial number components Content (mg / L) serial number components Content (mg / L) 1 KNO 3 :

1900 11 CuSO 4 .5H 2 o

0.025 2 NH 4 NO 3

1650 12 CoCL 2 .6H 2 o

0.025 3 MgSO 4 ·7H 2 o

370 13 Na 2 -EDTA

37.3 4 K H 2 PO 4

170 14 FeSO 4 ·7H 2 o

27.8 5 CaCl 2 2H 2 o

440 15 Glycine 2.0 6 MnSO 4 4H 2 o

22.3 16 Pyridoxine Hydrochloride 0.5 7 ZnSO4·7H 2 o

8.6 17 Ammonium sulfate hydrochloride 0.1 8 h 3 BO 3

6.2 18 niacin 0.5 9 KI 0.83 19 Creatine 100 10 Na 2 MoO 4 ·7H 2 o

...

Embodiment 2

[0048] Step 1: Prepare primary medium, proliferation medium and rooting medium

[0049] 1. The first generation, proliferation and rooting medium prepared in the present embodiment are the MS medium that is adopted as the base medium, and the formulation of the MS medium is as shown in Example 1.

[0050] 2. Preparation of primary culture medium

[0051] Add 6g of agar, 20g of sucrose, 1.0mg of kinetin (KT), and 0.05mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; aliquot Put it into the tissue culture bottle, and the height of the culture medium is 1-1.5cm, which is the best.

[0052] 3. Proliferation medium preparation

[0053] Add 6g of agar, 20g of sucrose, 2.5mg of 6-aminopurine (6BA), and 0.75mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Put...

Embodiment 3

[0067] Step 1: Prepare primary medium, proliferation medium and rooting medium

[0068] 1. The first generation, proliferation and rooting medium prepared in the present embodiment are the MS medium that is adopted as the base medium, and the formulation of the MS medium is as shown in Example 1.

[0069] 2. Preparation of primary culture medium

[0070] Add 10g of agar, 10g of sucrose, 1.5mg of kinetin (KT), and 0.5mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20 minutes before use; aliquot Put it into the tissue culture bottle, and the height of the culture medium is 1-1.5cm, which is the best.

[0071] 3. Proliferation medium preparation

[0072] Add 10g of agar, 10g of sucrose, 1.75mg of 6-aminopurine (6BA), and 1.0mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Put it ...

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