A kind of tissue culture method of ornamental aquatic plant Antarctica

A tissue culture and Antarctic technology, applied in the field of plant tissue culture, can solve the problems of Antarctica difficult to reproduce, slow propagation speed, and difficulty in obtaining plant seedlings, etc., and achieve the effect of short tissue reproduction cycle, convenient operation, effective and rapid asexual reproduction

Active Publication Date: 2022-03-22
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is easy to operate, easy to operate, and high in output, and overcomes the problems that Antarctica fir is not easy to propagate in the aquarium, the propagation speed is slow, and it is difficult to obtain plant seedlings.

Method used

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  • A kind of tissue culture method of ornamental aquatic plant Antarctica

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1: Prepare primary culture medium, proliferation medium and rooting medium

[0027] 1. The first-generation culture medium, proliferation medium and rooting medium used in this embodiment all adopt MS medium as the basic medium, and the formula of MS medium is as follows: mg / L

[0028] Table 1 MS medium formula

[0029] serial number components Content (mg / L) serial number components Content (mg / L) 1 KNO 3 :

1900 11 CuSO 4 .5H 2 o

0.025 2 NH 4 NO 3

1650 12 CoCL 2 .6H 2 o

0.025 3 MgSO 4 ·7H 2 o

370 13 Na 2 -EDTA

37.3 4 K H 2 PO 4

170 14 FeSO 4 ·7H 2 o

27.8 5 CaCl 2 2H 2 o

440 15 Glycine 2.0 6 MnSO 4 4H 2 o

22.3 16 Pyridoxine Hydrochloride 0.5 7 ZnSO4·7H 2 o

8.6 17 Ammonium sulfate hydrochloride 0.1 8 h 3 BO 3

6.2 18 niacin 0.5 9 KI 0.83 19 Creatine 100 10 Na 2 MoO 4 ·7H 2 o

...

Embodiment 2

[0048] Step 1: Prepare primary medium, proliferation medium and rooting medium

[0049] 1. The first generation, proliferation and rooting medium prepared in the present embodiment are the MS medium that is adopted as the base medium, and the formulation of the MS medium is as shown in Example 1.

[0050] 2. Preparation of primary culture medium

[0051] Add 6g of agar, 20g of sucrose, 1.0mg of kinetin (KT), and 0.05mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; aliquot Put it into the tissue culture bottle, and the height of the culture medium is 1-1.5cm, which is the best.

[0052] 3. Proliferation medium preparation

[0053] Add 6g of agar, 20g of sucrose, 2.5mg of 6-aminopurine (6BA), and 0.75mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Put...

Embodiment 3

[0067] Step 1: Prepare primary medium, proliferation medium and rooting medium

[0068] 1. The first generation, proliferation and rooting medium prepared in the present embodiment are the MS medium that is adopted as the base medium, and the formulation of the MS medium is as shown in Example 1.

[0069] 2. Preparation of primary culture medium

[0070] Add 10g of agar, 10g of sucrose, 1.5mg of kinetin (KT), and 0.5mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20 minutes before use; aliquot Put it into the tissue culture bottle, and the height of the culture medium is 1-1.5cm, which is the best.

[0071] 3. Proliferation medium preparation

[0072] Add 10g of agar, 10g of sucrose, 1.75mg of 6-aminopurine (6BA), and 1.0mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Put it ...

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Abstract

The present invention relates to a kind of tissue culture method of ornamental aquatic plant Antarctica, comprising the following steps: (1) pretreatment; (2) cutting the pretreated explant into 1-1.5cm long explant, each cut Each explant has at least one axillary bud; the explant after cutting is inserted into the first-generation culture medium and carried out the first-generation culture; (3) separate the first-generation adventitious buds in the first-generation medium, so that the length of the first-generation adventitious buds is controlled within 2cm; The first-generation adventitious buds after that are transferred to the propagation medium for cultivation; (4) the plants with a length greater than 3-4cm in the propagation medium are transferred to the rooting medium for cultivation; Take it out from the base, rinse it with clean water and transplant it into an ecological tank. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, and quickly establish an aseptic propagation system for Aratarctica. Seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of ornamental aquatic plant Antarctica. Background technique [0002] Antarctica is a commonly used ornamental aquatic plant. Antarctica is a submerged herb, plantaceae, dicotyledons, emergent aquatic plants. In the wild, every flowering period, the water surface is as beautiful as a sea of ​​flowers, and you can also see it blooming in a plant tank. This kind of aquatic plants can adapt to a wide range of water quality changes in the water, and can also grow under low light, but it is still better to cultivate under stronger light. Antarctica is more like a green feather in shape, the difference is that the former has needle-like leaves and the latter has pinnate leaves. The current propagation method of Antarctica fir is cutting and lateral bud propagation. [0003] However, there is still a lack of mature technology for large-scale ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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