A method for asexual reproduction of Red Umbrella

A technology of asexual reproduction and red umbrella, applied in the field of plant tissue culture, can solve the problems of difficult reproduction and slow reproduction, and achieve the effects of short tissue reproduction cycle, convenient operation, and effective and rapid asexual reproduction.

Active Publication Date: 2022-04-15
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is easy to operate, easy to operate, high in output, and overcomes the problems of difficult reproduction and slow reproduction speed in aquariums.

Method used

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  • A method for asexual reproduction of Red Umbrella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step 1: Prepare induction medium and proliferation medium

[0023] 1. The induction medium and proliferation medium used in this example are all MS medium adopted as the base medium, and the formula of MS medium is as shown in Table 1:

[0024] Table 1: MS Medium Recipe

[0025]

[0026] 2. Preparation of induction medium

[0027] The induction medium is MS-based medium, and 6-aminopurine (6-BA), agar powder, sucrose, activated carbon and plant tissue antibacterial agent (PPM) are added; the composition concentration of the induction medium is: MS basal medium 11.74g / L, 1mg / L 6-BA, 7.5g / L agar powder, 30g / L sucrose, 4g / L activated carbon, 1ml / LPPM.

[0028] 3. Proliferation medium preparation

[0029] The proliferation medium is based on MS as the base medium, and 6-BA, indole acetic acid, agar powder and sucrose are added; the component concentration of the proliferation medium is: MS base medium 11.74g / L, 1mg / L 6-BA , 0.5mg / L indole acetic acid, 7.5g / L agar pow...

Embodiment 2

[0040] Step 1: Prepare Induction Medium and Proliferation Medium

[0041] 1. The induction and proliferation medium prepared in this example is the MS medium used as the basal medium, and the formulation of the MS medium is the same as that shown in Example 1.

[0042] 2. Preparation of induction medium

[0043] The induction medium is MS-based medium, and 6-aminopurine (6-BA), agar powder, sucrose, activated carbon and plant tissue antibacterial agent (PPM) are added; the composition concentration of the induction medium is: MS basal medium 11.74g / L, 1.5mg / L 6-BA, 6g / L agar powder, 30g / L sucrose, 4g / L activated carbon, 1ml / LPPM.

[0044] 3. Proliferation medium preparation

[0045] The proliferation medium is based on MS as the base medium, and 6-BA, indole acetic acid, agar powder and sucrose are added; the component concentration of the proliferation medium is: MS base medium 11.74g / L, 5mg / L 6-BA , 0.75mg / L indole acetic acid, 6g / L agar powder and 30g / L sucrose.

[0046...

Embodiment 3

[0056] Step 1: Prepare Induction Medium and Proliferation Medium

[0057] 2. The induction and proliferation medium prepared in this example is the MS medium used as the basal medium, and the formula of the MS medium is the same as that shown in Example 1.

[0058] 2. Preparation of induction medium

[0059] The induction medium is MS-based medium, and 6-aminopurine (6-BA), agar powder, sucrose, activated carbon and plant tissue antibacterial agent (PPM) are added; the composition concentration of the induction medium is: MS basal medium 11.74g / L, 2mg / L 6-BA, 8g / L agar powder, 30g / L sucrose, 8g / L activated carbon, 1ml / LPPM.

[0060] 3. Proliferation medium preparation

[0061] The proliferation medium is based on MS as the base medium, and 6-BA, indole acetic acid, agar powder and sucrose are added; the component concentration of the proliferation medium is: MS base medium 11.74g / L, 2.5mg / L 6- BA, 1mg / L indole acetic acid, 8g / L agar powder and 30g / L sucrose.

[0062] Step ...

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Abstract

The present invention relates to a kind of asexual reproduction method of red umbrella, comprising: (1) explant pretreatment; (2) cutting the explant after pretreatment into several small explants, each small explant belt There are 1-3 stem nodes, and the small explants are inoculated on the induction medium and cultivated until the clustered buds form; (3) separate the clustered buds in the induction medium, so that the clustered shoots length is controlled at 2-3cm; The clumps of shoots were inoculated into the proliferation medium and cultivated. When the new shoots grew to 2-3cm, the new shoots were separated and inserted in the proliferation medium for repeated subculture for 3-4 times to obtain a large number of clumps of shoots after propagation; (4) Split the clustered buds with a length of 3-5 cm from the base of the clustered buds after multiplication, cut them and plant them. This method can obtain a large number of new plants without being affected by seasons, temperatures, and regions, so as to quickly establish a sterile propagation system for red umbrellas, provide technical support for maintaining the excellent seed properties of red umbrellas, and provide a steady stream of sterile breeding for the majority of fans. Bacteria seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for asexual reproduction of red umbrellas. Background technique [0002] Red Umbrella, dicotyledonous plant, Xiao Erxian grass family, emergent aquatic plants. There are different types of water grass and water grass. Water grass has lanceolate alternate leaves, leaf color is emerald green, leaf edge has serration. Cultivated in water, there will be many different changes. Including leaves can change from lanceolate to oval, irregularly split leaves, and even pinnate leaves. The color can change from green to orange-red to deep red. It varies according to the cultivation conditions, and it has a variety of forms. It likes strong light and low water temperature, and grows slowly in water. Rare things are more expensive, so red umbrellas are often regarded as classics among red grasses by aquatic plant players, and red umbrellas can change into different...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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