Barbadosnut plantlet tissue culture rapid propagation and rooting method

Abstract

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h / d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

Description

technical field

[0001] The invention belongs to the technical field of Jatropha curcas plant tissue culture rapid propagation, in particular to a method for tissue culture rapid propagation and rooting of Jatropha curcas micro seedlings. technical background

[0002] Jatropha curcas is a plant of the Euphorbiaceae Jatropha genus, mainly distributed in tropical and subtropical regions. Jatropha curcas has the effect of dispelling silt and relieving pain, and is often used to treat bruises, skin itching and gastroenteritis. In recent years, studies have found that Jatropha curcas not only contains anti-cancer, disease-resistant, and insect-resistant ingredients, which has potential important value in the development of new drugs, but also has a high oil content, and its oil composition is close to that of petrochemical diesel oil. Jatropha curcas is resistant to drought and barrenness, and has low environmental requirements. Therefore, Jatropha curcas can be used as an energy...