57results about How to "Single type" patented technology

The invention relates to a method for separating and culturing human chorionic mesenchymal stem cells, belonging to the technical field of biomedicine. The present invention obtains mesenchymal stem cells and in vitro culture and freezing of mesenchymal stem cells through the collection of human placental chorionic tissue, stripping of chorionic tissue, disinfection, washing, shredding, primary separation, and the isolated cells are related detection. The invention can separate and obtain a large number of mesenchymal stem cells with uniform and stable phenotype from human placental chorionic tissue, which meets clinical application standards through testing. The number of cells that can meet cryopreservation or clinical use can be obtained by culturing in vitro expansion.

The invention discloses a method for separating and purifying chlorogenic acid from eucommia bark leaves, which comprises the following steps of: extracting eucommia bark plant leaves serving as raw materials at the temperature of between 40 and 70 DEG C for 15 to 40 minutes by using 20 to 80 volume percent aqueous solution of a strongly polar organic solvent as an extractant with the aid of microwaves with the power of 300 to 800W, filtering extract, and drying to obtain a eucommia bark leaf flavonoid crude product, wherein the material-to-liquid ratio of the raw materials to the extractant is 1g:(10-50)mL; and performing high-speed countercurrent chromatographic separation and purification on the eucommia bark leaf flavonoid crude product by using ethyl acetate, n-butanol and water in avolume ratio of (3-4):1:(4-5) as a solvent system to obtain the chlorogenic acid. The invention has the main advantages that: operating steps are simple, the equipment type is single, the production period is short, product purity is high, and the method has high application value and good social and economic benefits.

The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.

The invention discloses a barbadosnut plantlet tissue culture rapid propagation and rooting method, which comprises the steps as follows: a germ of a barbadosnut seed after disinfection and sterilization is placed in a basic culture medium with the pH value of 5.4 to 6.0, and cultured to germinate into a sterile plantlet after 5 days at the culture temperature of 25 to 35 DEG C, with the illumination intensity of 1500 to 3000lx and the illumination time of 12h/d; stem segments with single bud of the sterile plantlet are placed in the rapid propagation culture medium, and cultured for 7days so as to induce out cluster buds; and the cluster buds growing to 2 to 3cm are cut and inoculate to a rooting culture medium, start to root after being cultured for another 5 days, and then grow into 2 to 3 cm strong stocks after 15 days, thus being capable of being transplanted. Only 66 days are needed for the rooting of the first propagation plantlet from the seed germination, therefore, the propagation is rapid; the hormone concentration of the culture process is low, the contact time is short, and the variation probability is low; 4.25 plantets can be obtained by utilizing one seed for 3 weeks, therefore, the propagation coefficient is high; and the types of used agents are single, the usage amount is small, the price is cheap and the cost is low.

The invention relates to a low-creep azorite indecomposable overflow brick and a preparation method thereof. The brick comprises the following raw materials in percentage by weight: 99.40-99.85% of azorite powder and 0.15-0.60% of ilmenite powder. The product prepared by the method provided by the invention is high in volume density and extremely low in apparent porosity, significantly reduces the high-temperature bending creep rate of an azorite material, and has an extremely-low foaming index to liquid crystal glass; and the preparation method is small in added quantity of a sintering aid, does not cause decomposition of azorite during firing, is simple and easy to implement, is scientific and reasonable, and is convenient for large-scale industrial production.

The invention discloses a tumble detection method and device. A first pressure signal, a second pressure signal and a third pressure signal are acquired in real time through a first pressure sensor arranged on the inner side of a shoe sole, a second pressure sensor arranged on the inner side of a shoe rear side part, and a third pressure sensor arranged on the inner side of a shoe heel part respectively; if the duration time of the acquired first pressure signal, second pressure signal and third pressure signal meeting the following conditions reaches a preset time threshold value: the first pressure signal is smaller than a first preset threshold value, or the second pressure signal is larger than a second preset threshold value, or the third pressure signal is larger than a third preset threshold value, it is judged that a tumble happens; a tumble alarm signal is given out under the condition that it is judged that the tumble happens. The tumble can be accurately detected, the implementation method is simple, and cost is low.

The invention discloses a raw bamboo multi-pipe beam space lattice structure system and a double-pipe clamp type connecting element series. A single double-pipe clamp comprises a left circular ring, a right circular ring and screw bolts; the left and right circular rings have the same or different dimensions, are respectively hooped on the outer wall of one bamboo pipe and are tightly hooped and fixed by screw bolts arranged at the two sides; the sides between the left circular ring and the right circular ring are connected through being fastened and hooped by the same screw bolt; the single bamboo pipe triangular shape is mainly formed by connecting three bamboo pipes as rod elements by a folding type double-pipe clamp; the raw bamboo multi-pipe beam space lattice structure is mainly formed by connecting a plurality of bamboo pipe triangles as installing units by the double-pipe clamp; the bamboo pipe or the bamboo pipe triangular shape can be sequentially and singly replaced through dismounting and mounting the double-pipe clamp; the structure repair is completed. The structure of the double-pipe clamp type connecting element is simple; the universality is high; the processing convenience is good; the connection reliability of the bamboo pipe and the double-pipe clamp is high; the integral structure bearing force is great; the building use comfort is good; the structure repair is convenient; the structure durability is high.

The invention provides a human placenta mesenchymal stem cell, a preparation method and an application thereof. The preparation method comprises the following steps: collecting, separating, culturing,cryopreserving, detecting, recovering, and the like. A high-purity high-activity mesenchymal stem cell can be acquired by only performing slide adherent culture after a human placenta chorion tissueblock is acquired in the separating process; the technological process is simplified; the cost of enzymic digestion is saved; the adherence and growth rate of primary cells are accelerated; the cell culture period is shortened; the introduction of more external interference factors is avoided, so that the process stability can be easily controlled. The multiplication capacity of the human placentamesenchymal stem cell acquired according to the invention is more stable than that of other mesenchymal stem cells; after the human placenta mesenchymal stem cell passes to P20 generation, the cell still can stably proliferate, and the cellular morphology, molecular surface antigen and adipogenesis osteogenesis differentiative potential thereof all meet the regulations for minimum standard of MSCidentification from international cell therapy association.

The invention discloses a Gannan potentilla anserine efficient planting method. The method comprises the steps of land selection, land preparation and fertilization, plant disease and insect pest controlling and digging at a proper time; the planting method is characterized by five aspects of a planting season and seed selection, planting pattern, planting density and seeding quantity, an irrigation method and irrigation quantity and foliage application; according to an efficient planting technology of potentilla anserine in areas in hostile natural conditions which is high and cold and high in altitude and the like, scale and standardized potentilla anserine planting in the Gannan plateau area is achieved, the potentilla anserine industry is developed, and service guarantee is provided for economy development, targeted poverty alleviation, and income increase of farmers and herdsmen in high and cold areas in hostile natural environment conditions.

The invention provides a catalyst for synthesizing 3-buten-1-ol through 1,4-butanediol and a preparation method of the catalyst. The catalyst is a CaO-SiO2-ZrO2 catalyst prepared by combining sol-gel with deposition-precipitation through a one-pot process, wherein the content of CaO in the catalyst is 5-25wt%, and the content of SiO2 is 0-15wt%. The preparation method comprises the following steps: hydrolyzing a silicon source, hydrolyzing a zirconium source, gelling silicon-zirconium, performing calcium source deposition-precipitation and performing aftertreatment. The preparation method is simple, the operation is convenient, and large-scale production is easily realized. According to the prepared catalyst, 1,4-butanediol is catalyzed to perform selective dehydration to synthesize 3-buten-1-ol under the reaction condition of the temperature of 300-400 DEG C, the yield is 90 percent or above, and fewer reaction byproducts are produced and are easy to separate.

The invention discloses a mold used for manufacturing fan blade shearing ribs of a wind generating set, which comprises a mold die cavity and a turning edge. The invention is characterized in the mold is made of steel. The mold used for manufacturing the fan blade shearing ribs of the wind generating set of the invention uses the steel mode, so the mold manufacture work procedure is simple, the materials have single type, the manufacture cost is lower, in addition, the later period maintenance and conservation cost is low, and the correction after the occurrence of deformation is easy.

The invention discloses a preparation method of a two-dimensional carbon-rich material rich in carbonyl (double bonds of carbon to oxygen), thioketone (double bonds of carbon to sulphur) and selenone (double bonds of carbon to selenium) functional groups and application of the two-dimensional carbon-rich material, and belongs to the field of application of the material. The material consists of conjugated six-membered rings containing the carbonyl, thioketone and selenone functional groups and acetylenic bonds; 1 and 4 positions of each conjugated six-membered ring are substituted by the carbonyl, thioketone and selenone functional groups; 2, 3, 5 and 6 positions of each conjugated six-membered ring are connected with the acetylenic bonds; and two acetylenic bonds exist between each two adjacent six-membered rings to form a highly conjugated two-dimensional planar framework structure. The preparation method mainly comprises the following steps: taking materials such as tetra-alkynyl substituted p-benzoquinone as a polymeric precursor; carrying out alkyne coupled reaction in a dioxane solution so as to obtain the carbon-rich two-dimensional material with the highly conjugated structure. Owing to introduction of carbonyl, double bonds of carbon to sulphur and double bonds of carbon to selenium, an ion transport channel and an energy storage activity points of the two-dimensional carbon-rich material are improved effectively, and therefore, the two-dimensional carbon-rich material has good application prospect in the field of energy storage devices.

The invention discloses an officinal Gannan potentilla anserine planting method. The officinal Gannan potentilla anserine planting method includes the steps of land selection, soil preparation and fertilization. The key technology of the planting method includes the steps of the planting stage and seed selection, the planting mode, the planting density and seeding rate, the irrigation mode and irrigation volume and foliage application; disease and insect damage preventing and controlling and timely digging are adopted. According to the efficient potentilla anserine planting technology for the high and cold high-altitude regions with bad natural conditions, potentilla anserine can be planted in the Gannan plateau region in a standard mode at a large scale, the potentilla anserine industry is developed, and service assurance is provided for economy developing, targeted poverty alleviation, targeted poverty overcoming and farmer and herdsmen income increasing of high and cold region with bad natural environmental conditions.

The invention provides a system for calcining treatment and resource utilization of sugar-making filter mud. The system comprises a pulverized coal preparation system, a feeding system, a calcining system, a waste heat recovery system, a flue gas environment-friendly up-to-standard emission treatment system and a quick lime recovery system, Raw material coal is prepared into pulverized coal as auxiliary fuel, crude sugar filter mud is crushed, the pulverized coal and the crude sugar filter mud are simultaneously added into a calcining furnace, ash generated after calcining is discharged and cooled to serve as a quick lime material; flue gas generated after calcination enters a waste heat boiler to be subjected to heat recycling, saturated steam is generated, the flue gas obtained after heat exchange enters a denitration device to be subjected to denitration treatment, the flue gas obtained after denitration exchanges heat with air and then is subjected to dust removal, and clean flue gas is obtained; and air heated by the flue gas is introduced into the calcining furnace as combustion-supporting air, so that the temperature of the combustion-supporting air entering the calcining furnace is integrally increased. Reduction and harmless treatment of the sugar-making filter mud are achieved, meanwhile, quick lime generated in the high-temperature calcination process, namely the high-temperature decomposition reaction process, is recycled to achieve the purpose of resource utilization, high-temperature flue gas waste heat generated in calcination is recycled to generate saturated steam, and the purpose of energy conservation in the environment-friendly treatment process is achieved.

The invention provides a system and a method for continuously preparing acetophenone, which are characterized in that ethylbenzene is used as a raw material and is sequentially subjected to at least one stage of rectification reaction; in each stage of rectification reaction, the material is fully mixed with part of gas phase to enter a reaction rectification tower and is in contact reaction with gas phase rising from the bottom in the tower, a mixed gas phase mainly containing water and ethylbenzene is extracted from the tower top, condensation and phase splitting are performed, an oil phase in condensate returns to the tower top, a water phase in the condensate is extracted as wastewater, and the water phase in the condensate is recycled. One part of the reaction liquid extracted from the tower kettle is fed into the next-stage rectification reaction, the other part of the reaction liquid is returned to the upper part of the reactive distillation tower as a circulating material through temperature control, and the part of the reaction liquid extracted from the tower kettle in the last-stage rectification reaction, which is not used as the circulating material, is extracted as a product. The method has the characteristics of good reaction selectivity, no generation of hazardous waste, energy conservation, environmental protection, continuous operation, easiness in large-scale production and the like.