Tissue culture method of wild sambucus williamsii

A tissue culture and elderberry technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of inability to maintain excellent seed properties, difficulty in seed harvesting, and easy mixing of varieties, and achieve the ability to maintain stress resistance. And ornamental, good stress resistance and ornamental, the effect of maintaining excellent variety

Inactive Publication Date: 2021-04-30
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that the Northeast elderberry asexual propagation method is in a blank stage, the seeds of the Northeast elderberry seed propagation method are difficult to harvest, the emergence rate is low and slow, the varieties are easy to mix, and the excellent seed properties cannot be maintained. Propose a tissue culture method of wild northeast elderberry

Method used

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  • Tissue culture method of wild sambucus williamsii
  • Tissue culture method of wild sambucus williamsii
  • Tissue culture method of wild sambucus williamsii

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specific Embodiment approach 1

[0036] A kind of tissue culture method of wild northeast elderberry of the present embodiment, such as figure 1 As shown, the method includes the following steps:

[0037] Step 1, the selection steps of explants:

[0038] Select the tender branches with dormant buds of the Northeast Elderberry in the current year, wash them with clean water, and select the stems with side buds as explants after hydroponics; the plant materials for the explants are taken from the Northeast Elderberry that grows vigorously and is free from diseases and insect pests wood plant;

[0039] Step 2, the disinfection steps of the explants:

[0040] Sterilize the explants sequentially with alcohol and mercuric chloride, cut off 2 mm at both ends of the sterilized explants, and keep the length of the stem segment at 1.5 cm to 3.5 cm;

[0041] Step 3, the step of inducing differentiation culture:

[0042] The explants after the disinfection in step 2 were inserted into the first-generation medium for ...

specific Embodiment approach 2

[0058] Different from the specific embodiment one, the tissue culture method of a kind of wild northeast elderberry of the present embodiment, the disinfection step of the explant in the described step 2 is specifically:

[0059] First, wash the surface of the explant with detergent to remove the dust on the surface, then rinse the explant with running water for 30 minutes, and then put it into a disinfection bottle;

[0060] After that, wipe the sterilized bottle containing the explant with 75% alcohol cotton and put it into the ultra-clean workbench, open the sterilized bottle containing the explant on the ultra-clean workbench, pour 75% alcohol into it and shake it for disinfection bottle, soaked and sterilized for 30s, poured off the alcohol, and rinsed the explants 3 to 5 times with sterile water;

[0061] After that, with 0.1% HgCl 2 Soak for 5 minutes, rinse with sterile water 4 to 5 times, and soak for 30 minutes, and then dry the moisture on the surface of the explan...

specific Embodiment approach 3

[0063] Different from the specific embodiment 1 or 2, in the tissue culture method of a kind of wild northeast elderberry of the present embodiment, in the disinfection step of the explant in step 2, the size of the explant also needs to be determined, specifically:

[0064] Remove the side branches and other useless parts of the collected branches, cut them into 4-5cm bud stem segments, put them in a beaker with washing powder water, cover the mouth of the beaker with a gauze net, shake the beaker continuously for 5 minutes, and put the Rinse the stem section beaker with running water for 1-2 hours.

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Abstract

The invention discloses a tissue culture method of wild sambucus williamsii and belongs to the field of plant tissue culture. An asexual reproduction method of the sambucus williamsii is in a blank stage. The tissue culture method of wild sambucus williamsii comprises the following steps: selecting a stem segment with a lateral bud as an explant; conducting disinfection and inoculating the disinfected explant into a primary culture medium for culturing to form the explant with a bud; inoculating the obtained explant with a bud into a proliferation culture medium for culturing; cutting off an adventitious bud and inoculating the adventitious bud into an MS culture medium for culturing; and and inoculating the cultured adventitious bud into a rooting culture medium for culturing. The method has advantages of having a high propagation coefficient, a short propagation period, and low cost and keeping good varieties.

Description

technical field [0001] The invention relates to a tissue culture method of wild northeast elderberry. Background technique [0002] Northeast elderberry (Sambucus mandshurica Kitag.), a deciduous shrub or small tree belonging to the genus Elderberry in Lonicaceae, is a unique tree species in Northeast China. [0003] Elderberry is a medicine and food homologous plant, which is named for its function of elderberry. Elderberry has a long history of medicinal use, which was first recorded in "Tang Materia Medica". Its twigs, fruits and flowers can be eaten; its roots, root bark, stems, leaves and flowers can be used as medicine. Moisture and other effects. Currently, many chemical compounds are isolated from the roots, stems, leaves and fruits of elderberry. In addition, the research on Elderberry plants has involved taxonomy, anatomy, cytology, micromorphology, physiology, reproductive technology, chemical composition analysis, development and utilization and other discipli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马盈
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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