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Method for quickly breeding plumeria rubra by tissue culture

A tissue culture, frangipani technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow reproduction speed, long cycle, inability to meet demand, etc., and achieve fast reproduction speed, easy operation, and uniform seedling emergence. Effect

Inactive Publication Date: 2013-12-18
武爱龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such a propagation speed is rather slow and the cycle is long, which is far from satisfying the demand for safflower frangipani seedlings in the domestic market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for rapid breeding of safflower frangipani by tissue culture, comprising the following steps:

[0026] A. Selection and disinfection of explants: After selecting the leaves on the mother plant of Frangipani frangipani with good growth, temporarily put them in a cool place indoors for two days. After the juice released by the leaves is dry, wash them with tap water for 10 minutes. After 10 minutes, dry it on a sterile ultra-clean table, then cut the leaves into small pieces of 2 cm in length and 2 cm in width, rinse the small pieces in 75% alcohol (5 seconds), and turn them into 0.1% Sterilize in mercuric solution (with five drops of Tween-20) for 10 minutes, rinse with sterile water for 3 times, cut off the leaves for later use;

[0027] B. Induction of explants: Inoculate the small pieces spared in step A on the callus induction medium, which is: MS+6-BA 3.0 mg / L+2,4-D 0.1 mg / L L+NAA 1 mg / L, 1.0% agar, 3% sucrose, 0.5% activated carbon and natural organic add...

Embodiment 2

[0034] A method for rapid breeding of safflower frangipani by tissue culture, comprising the following steps:

[0035] A. Selection and disinfection of explants: After selecting the leaves on the mother plant of Frangipani frangipani with good growth and strength, put them in a cool place indoors for two days. After the juice released by the leaves is dry, wash them with tap water for 10 minutes. After 10 minutes, dry it on a sterile ultra-clean table, then cut the leaves into small pieces of 3 cm in length and 3 cm in width, rinse the small pieces in 75% alcohol (10 seconds), and turn them into 0.2% Sterilize in mercuric solution (adding six drops of Tween-20) for 5 minutes, rinse with sterile water 4 times, cut off the leaves for later use;

[0036] B. Induction of explants: Inoculate the small pieces spared in step A on the callus induction medium, which is: MS+6-BA 2.0 mg / L+2,4-D 0.5 mg / L L+NAA 0.5 mg / L, 1.0% agar, 3% sucrose, 0.5% activated carbon and natural organic add...

Embodiment 3

[0043] A method for rapid breeding of safflower frangipani by tissue culture, comprising the following steps:

[0044] A. Selection and disinfection of explants: After selecting the leaves on the mother plant of Frangipani frangipani with good growth and strength, put them in a cool place indoors for two days. After the juice released by the leaves is dry, wash them with tap water for 10 minutes. After 10 minutes, dry it on a sterile ultra-clean table, then cut the leaves into small pieces of 3 cm in length and 3 cm in width, rinse the small pieces in 75% alcohol (8 seconds), and turn them into 0.2% Sterilize in mercuric solution (plus five drops of Tween-20) for 8 minutes, rinse with sterile water 4 times, cut off the leaves for later use;

[0045] B. Induction of explants: Inoculate the small pieces spared in step A on the callus induction medium, which is: MS+6-BA 2 mg / L+2,4-D 0.1 mg / L L+NAA 0.5 mg / L, 1.0% agar, 3% sucrose, 0.5% activated carbon and natural organic additiv...

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Abstract

The invention provides a method for quickly breeding plumeria rubra by tissue culture. The method comprises the following steps of carrying out tissue culture and rapid propagation with MS (Murashige and Skoog) as a minimal medium and plumeria rubra leaves as explants under different hormone components and concentration levels; carrying out sterilization treatment and inoculation induction to form a callus tissue, and then carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; and finally carrying out acclimatization and transplantation, wherein the minimal medium is based on the MS culture medium, and supplemented with components including 6-benzyl aminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the invention, tissue culture is applied to overcome the problem that the plumeria rubra is too long in seedling-raising period and low in propagation coefficient, so that factory-like quick seedling raising can be carried out for meeting the demands of the market on the plumeria rubra.

Description

technical field [0001] The invention relates to a method for rapid propagation of safflower plumeria by tissue culture. Background technique [0002] Frangipani, a small deciduous tree of the Apocynaceae family, also known as Gardenia Burmese, is native to tropical regions of America such as Mexico, and was first introduced into my country from Myanmar. Its petals are white and the flower center is light yellow, which looks like egg yolk wrapped in egg white, hence the name. Every April and May, the dignified and elegant Plumeria blooms one after another, with a rich and refreshing aroma. Five petals are stacked in a circle, which looks like a paper pinwheel folded by children. In fact, in addition to the white frangipani, there are also two types of red and yellow, both of which can be used to extract essence for the manufacture of high-grade cosmetics, soaps and food additives. The price is quite high and has great potential for commercial development; After drying, it ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 武爱龙
Owner 武爱龙
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