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Efficient regeneration method with zenia insignis leaves being explants

A high-efficiency regeneration and explant technology, applied in the field of plant tissue culture, can solve the problems that tissue culture has not yet been reported, and achieve the effect of strong repeatability, convenient operation and wide range of materials

Active Publication Date: 2017-01-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are some reports on the tissue culture research of Rendou in China, mainly focusing on the use of the hypocotyls of the aseptic seedlings of Rendou, the induction culture of the tender stems of the seedlings and the annual branches, and the other parts of the aseptic seedlings of Rendou and the cultivation of various clones. Tissue culture has not been reported

Method used

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  • Efficient regeneration method with zenia insignis leaves being explants
  • Efficient regeneration method with zenia insignis leaves being explants
  • Efficient regeneration method with zenia insignis leaves being explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Step 1: Seed pretreatment. Put the seeds in a water bath and soak them in a water bath at 85°C for 12 minutes. Finally, spread the seeds on a petri dish with wet filter paper, and keep the filter paper wet all the time. After the seeds were placed in the petri dish for 24 hours, healthy and obviously swollen seeds were selected for the next test operation.

[0034] Step 2: Disinfect and sterilize the seeds. On a sterile ultra-clean bench, sterilize with 75% alcohol for 28 seconds, rinse with sterile water once; sterilize with 0.15% mercuric chloride for 8 minutes, and rinse thoroughly with sterile water for 5 times. Seeds were sown in blank (without any hormone) MS medium for cultivation at a cultivation temperature of 25±2°C, light intensity of 1500lx, and light duration of 15h. Seed germination was observed, and the germination rate was 96.54%.

[0035] Step 3: Obtain tissue culture materials. After 7 days, it was found that the seeds began to germinate, and the ...

Embodiment 2

[0039] Step 1: Seed pretreatment. Put the seeds in a water bath and soak them in a water bath at 90°C for 8 minutes. Finally, spread the seeds on a petri dish with wet filter paper, and keep the filter paper wet all the time. After the seeds were placed in the petri dish for 24 hours, healthy and obviously swollen seeds were selected for the next test operation.

[0040] Step 2: Disinfect and sterilize the seeds. On a sterile ultra-clean bench, sterilize with 75% alcohol for 30 seconds, rinse with sterile water once; sterilize with 0.1% mercuric chloride for 10 minutes, and rinse thoroughly with sterile water for 5 times. Seeds were sown in blank (without any hormone) MS medium for cultivation at a cultivation temperature of 25±2°C, light intensity of 2000lx, and light duration of 12h. Seed germination was observed, and the germination rate was 98%.

[0041] Step 3: Obtain tissue culture materials. After 7 days, it was found that the seeds began to germinate, and the hypo...

Embodiment 3

[0045] Step 1: Seed pretreatment. The seeds were placed in a water bath and soaked in a water bath at 88°C for 10 min. Finally, spread the seeds on a petri dish with wet filter paper, and keep the filter paper wet all the time. After the seeds were placed in the petri dish for 24 hours, healthy and obviously swollen seeds were selected for the next test operation.

[0046] Step 2: Disinfect and sterilize the seeds. On a sterile ultra-clean bench, sterilize with 75% alcohol for 25 seconds, rinse with sterile water once; sterilize with 0.2% mercuric chloride for 5 minutes, and rinse thoroughly with sterile water for 5 times. Seeds were sown in a blank (without any hormone) MS medium for culture, the culture temperature was 25±2°C, the light intensity was 2500lx, and the light time was 10h. Seed germination was observed, and the germination rate was 95%.

[0047] Step 3: Obtain tissue culture materials. After 7 days, it was found that the seeds began to germinate, and the hy...

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Abstract

The invention discloses an efficient regeneration method with zenia insignis leaves being explants and belongs to the technical field of plant tissue culturing. With the leaves being materials, a zenia insignis regeneration system is researched for improvement in the regeneration capacity of zenia insignis, and an efficient regeneration system is established and used for providing effective reference value for genetic resource conservation and genetic engineering research later. The efficient tissue culturing and plant regeneration method can be free of influences of external conditions, and robust, neat and uniform zenia insignis tissue culture seedlings are produced on a large scale in an industrialized mode. The zenia insignis leaves serve as the drawn materials, and compared with the reported mode that hypocotyls and annual branches serve as explants to construct a regeneration system, the efficient regeneration method has the advantages of being convenient to operate, wide in range of drawn materials, high in repeatability and efficiency and the like. The method not only can meet the requirement for seedlings of the market, but also can modify and innovate zenia insignis genetic resources through transgene research to lay a foundation for sustainable utilization of the zenia insignis genetic resources.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency regeneration method using bean leaves as explants. Background technique [0002] Zenia insignis Chun (Zenia insignis Chun), also known as Zenia insignis Chun, is a fast-growing deciduous tree belonging to the Hemataceae family. Rendou is a rare tree species in my country, a single genus species, and belongs to the second-class protected wild tree species in my country. Rendou species are mainly distributed in tropical Asia-India-Malaysia-Vietnam (or Indochina Peninsula) to South China (or Southwest) regions, and are mainly distributed in Hunan, Guangdong, Guangxi, Yunnan, Guizhou and other provinces in China. Rendou has strong germination and renewal ability, rapid growth, and early forestry and timber growth. Its wood material is excellent and can be used as fuel; its root system is developed, its resistance is strong, its flowers and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈晓阳周宇晴张俊杰林孟飞
Owner SOUTH CHINA AGRI UNIV
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