37results about How to "Solve reproductive problems" patented technology

The invention discloses a fence preservation method for prolonging shelf life of cold fresh poultry. The fence preservation method for prolonging the shelf life of the cold fresh poultry comprises the following steps of: placing fresh poultry which is obtained by slaughtering, washed completely and cut into a refrigeration storage at the temperature of between 2 and 5 DEG C and precooling the poultry for 1 to 2 hours, so that the surface temperature of the poultry is reduced below 5 DEG C and the internal temperature of the poultry is reduced below 10 DEG C; transferring the precooled poultry to a purification room and performing surface sterilization on the poultry through ozone or ozone solution of an ozone generator; immersing the poultry subjected to ozone sterilization into chitosan glutamate solution to retain freshness; taking out and draining the poultry; placing the poultry after corrosion prevention and freshness preservation into a food plastic packaging box; introducing carbon dioxide (CO2) and nitrogen (N2) into the food plastic packaging box and performing sealing package; and storing the packaged poultry into a cold chain at the temperature of between 2 and 5 DEG C. The fence preservation method for prolonging the shelf life of the cold fresh poultry has good effect and is suitable for retaining freshness of the fresh cut poultry.

The invention discloses an application of soluble chitose as oral article, which is characterized by the following: sterilizing Gram-positive bacterium and negative bacterium due to 0.1-20% soluble chitose; possessing affinity with oral bacteria and oil anion; repairing ulcer; accelerating microcirculation of surface capillary; augumenting the response of immune cell in the deep layer.

The invention discloses a method for generating and cultivating papuan jellyfish ephyrae and belongs to the field of artificial feeding and breeding of ornamental marine animals. A papuan jellyfish hydranth transversely cracks to generate an ephyra every time, the ephyra is continuously cultivated for 5 to 10 days, and second-time single-ephyra transverse cracking is generated; the ephyra which just cracks is bred in a container where bubbles drive water flow, one to three artemia nauplii are fed with each milliliter of water every day, and the water is changed after the artemia nauplii are fed for 2h. Cultivation conditions are as follows: the water temperature is 20 to 27 DEG C, 8000Lux 8h illumination 16h dark alternation is carried out, 5 to 10d ephyrae are transformed into jellyfish bred bodies and the jellyfish bred bodies are moved into a circulation cylinder where a water pump drives the water flow. According to the method, transverse cracking of the papuan jellyfish hydranth is promoted in an artificial environment, the ephyrae are generated and are further cultivated to obtain the jellyfish bred bodies and adults, and the situation that exhibition in an aquarium is maintained by virtue of fishing is solved.

The invention discloses a wheat fertility recovery gene and an application thereof, belongs to the field of plant biotechnology, and in particular relates to a recessive genic male sterility gene and cloning of a promoter of the gene as well as an application of the gene in crossbreeding. Through flow cytometry and a high-throughput sequencing method, the fertility recovery gene FRG1 is successfully cloned; and with the application of the gene, male fertility of a Lanzhou genic male sterile mutant or an allelic mutant thereof can be completely recovered, so that a foundation is laid for the construction of a novel wheat crossbreeding technique system. Meanwhile, more possibilities are offered for solving such technical bottleneck problems in existing 'three-line' and 'two-line' wheat crossbreeding technologies which are unstable in sterile line fertility, limited in resource of hybrid varieties, complex in seed production technique, high in seed production cost and the like. The gene and sterile line propagation and maintaining method provided by the invention has great significance and application value of wheat crossbreeding work.

The invention discloses a method for cutting and raising seedlings of tutcheria hexalocularia employing a container. An offspring plant container seedling which is kept consistent with an excellent single strain in inheritable characters is obtained in manners of selecting an excellent single strain of the tutcheria hexalocularia as a stock plant by an inventor; selecting a nursery land, and preparing soil; preparing and sterilizing matrix; collecting, cutting and processing cuttings; moisturizing, shading, managing after rooting, and the like. Blooming is earlier than sowing seedling, and the cutting survival rate and the transplanting survival rate are high. The method disclosed by the invention is simple to operate, low in production cost and applicable to vegetative propagation of excellent single strain of the tutcheria hexalocularia; the difficulties of propagating and transplanting of the excellent single strain of the tutcheria hexalocularia can be effectively solved; the method can be applied to massive cultivation of seedlings of rare plants and excellent ornamental tree tutcheria hexalocularia; the difficulty of seedling of the key protected wild plant, namely the tutcheria hexalocularia in Guangxi can be broken through; protection of the rare plant tutcheria hexalocularia resource, and application and promotion thereof in landscaping are facilitated.

The invention discloses a method for killing escherichia coli and especially discloses a use of a freshly squeezed red bayberry juice in killing of escherichia coli. According to the invention, a freshly squeezed red bayberry juice is added into a liquid containing escherichia coli, and the mixture is uniformly mixed and then is subjected to pressure maintaining treatment under the pressure greater than or equal to 10MPa for 1-1.5min, wherein the volume of the freshly squeezed red bayberry juice is at least 1 time volume of the liquid containing escherichia coli. The method provided by the invention can greatly reduce pressure requirements under high-pressure sterilization conditions and avoid microbe follow-up breeding in a storage period after sterilization.

The invention belongs to the technical field of plant storage and discloses a brachyceras overwintering storage and propagation method. The method includes the steps of picking of dormant stems, wherein one week after arrival of first frost, brachyceras plants growing in the open air are dug out, leaves and root systems on the plants are removed, the formed dormant stems are acquired, and soil and impurities on the dormant stems are flushed away with clear water; air-drying of the dormant stems, wherein the dormant stems are arranged at indoor shady, cool and ventilating positions and dried in the shade till the surfaces are dry; storage of the dormant stems, wherein the air-dried dormant stems are put in plastic self-sealing bags, bag openings are sealed, and the dormant stems are subjected to overwintering storage under the light-shading and indoor 4.5-16 DEG C temperature varying conditions. The invention further relates to a method for propagating brachyceras through dormant balls. The method facilitates material obtaining, is easy to operate, saves cost, solves the problem about overwintering storage of the brachyceras in the northern areas of China, and provides experimental bases for further application and popularization of the brachyceras in northern landscape architecture.

The process of screening nematocidal Bacillus thuringiensis strain includes the steps of soaking bioassay, culture medium bioassay, protein crystal bioassay, etc. The process adopts easy-to-obtain defect type colibacillus JM109, and has short screening period, low cost, high screening efficiency. The screened nematocidal Bacillus thuringiensis strain IPS has a 24 hr LC50 of 1.008 mcg/ml.

The invention discloses a rapid propagation method for hemp seeds. The method includes the following steps that the screened hemp seeds are put into 50% carbendazim wettable powder with the concentration of 0.2% to be subjected to immersion disinfection, and moisture on the hemp seeds is drained off; the hemp seeds are immersed in 30-35 DEG C warm water for 18-24 h, and moisture on the hemp seeds is drained off; 50-70-mesh perlite is taken and heated for 10-15 min in a 150-160 DEG C drying oven and then naturally cooled, clean water is added to wet the perlite, and treated perlite is obtained; the treated perlite is laid in a box with holes in the bottom, the immersed hemp seeds are evenly scattered on the perlite, and another layer of perlite is laid; the box is irradiated for 10-24 h every day through an infrared thermal insulation lamp and placed in a 28-30 DEG C environment in the remaining time every day, and watering is performed once every day. The method is easy and fast to implement and low in cost, has great economic benefits and social benefits and provides reliable guarantees for large-scale industrial planting of the hemp seeds.

The invention discloses a method for synergistically killing staphylococcus aureus through citruse naringenin and a high-strength pulsed electric field. The method comprises the following steps of adding naringenin to a liquid food containing staphylococcus aureus, to be sterilized, and performing pretreatment; and preforming sterilization on materials pretreated with the naringenin by the high-strength pulsed electric field. Preferably, the addition concentration of the naringenin is 12.5-125mg/L, the pretreatment time is 0.5-1.0h, the parameters of the pulsed electric field are set as that the electric field strength is 20-50kV/cm, the pulse width is 10-40[mu]s, the pulse frequency is 0.1-1.0kHz, and the total time that materials are subjected to pulsing treatment is 60-1000[mu]s. According to the method disclosed by the invention, a natural antibacterial ingredient namely the naringenin is combined with a high-strength pulsed electric field sterilization technique, so that the effect of killing the staphylococcus aureus is notably increased, the food is safe, the storage period is prolonged, and the loss of original flavor and original nutrient substances is reduced.

The invention provides an artificial seedling raising method for black rosewood. The method comprises the steps of seed treating, wherein seeds are collected, seed treating liquid is prepared, the black rosewood seeds, the seed treating liquid and sandy soil are mixed to be uniform and laid in a seed culture pot, the seed culture pot is put in a greenhouse, germination accelerating is conducted, and black rosewood bud seedlings are obtained; land selecting for nursery land building, wherein nursery land is selected and treated; bud seedling transplanting; seedling stage managing, wherein seedling stage managing is conducted after bud seedling transplanting, and sunshading managing, water managing, fertilization managing, weeding managing and disease and pest preventing and controlling are conducted. According to the method, the high survival rate of black rosewood seed seedling raising is achieved, and the black rosewood planting difficulty is reduced; meanwhile, the condition that in a traditional planting mode, black rosewood nursery stocks are nonuniform and irregular is improved, and the nursery stock quality is improved.

The invention discloses a foamless sterilization remover for air separation recirculated cooling water. The above water treatment agent comprises 2-20% of acrylic acid-acrylamido-2-methylpropanesulfonic acid-acrylate multipolymer, 5-30% of polyquatemary ammonium salt, 2-20% of sodium polyepoxysuccinate, 5-30% of polyhexamethylene guanidine and 10-50% of water. The water treatment agent can solve the problems of microbe breeding and biological slime deposition of air separation and oxygen production systems in order to effectively overcome insufficiencies of existing water treatment technologies and realize the energy saving and emission reduction purposes.

The invention discloses an active soil for purifying high concentration polluted air in tunnels or other local areas. The active soil contains microorganisms used for decomposing harmful gas in tail gas and is characterized by also including topsoil, perlite, pumice, turf, muck and artificial zeolite, wherein the volume ratio of topsoil to perlite to pumice to turf to muck is 4:2:1:1:1. The soil formula can solve the contradiction between the growth and reproduction of microorganisms and the physicochemical property of soil and ensure the activity of microorganisms in soil. The proportioning of ingredients causes the soil to have good air permeability, and the polluted gas can be evenly distributed in soil, thus further ensuring the utilization rate of the polluted gas by microorganisms. With higher purification efficiency of the polluted gas, the active soil saves use cost and can eliminate or abate the pollution to the atmosphere by high concentration vehicle tail gas in tunnels to the maximum degree.