Method for screening Bacillus thuringiensis nematocidal strain

A Bacillus thuringiensis, nematicidal technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the impact of nematicidal strain discovery and research speed, complicated procedures and techniques, lack of fast, simple, accurate and efficient. Economical bioassay methods, etc., to achieve the effect of good control effect, cost saving, and improvement of screening efficiency

Inactive Publication Date: 2008-03-26
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

Nematode artificial breeding and drug efficacy determination procedures and techniques are complicated, and there is a lack of rapid, simple, accurate and economical bioassay methods like the plate inhibition zone method commonly used in antibacterial substance screening, which limits the discovery and research speed of nematicidal strains. certain influence

Method used

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Embodiment

[0019] Embodiment: The screening method of Bacillus thuringiensis nematode highly virulent strains, with Bt as the test strain, with Caenorhabditis elegans as the target pest, using soaking bioassay, medium bioassay and protein crystal bioassay to screen Bacillus thuringiensis Nematode strains:

[0020] 1. Bioassay by immersion: take eggs and larval stage nematodes as target pests, centrifuge the overnight culture solution of Bt bacterial strains at 1000r / min, and carry out indoor infection test on the supernatant; the aqueous suspension containing 100 healthy nematodes is mixed with equal volumes of waiting The test bacteria solution was fully mixed on the concave glass slide, and deionized water was used as a control, and the nematodes were kept moist for 24 hours at 25°C for 24 hours to determine whether the nematodes were dead or alive, and each treatment was repeated 4 times, and the Bt primary screening strains with strong nematicidal activity were selected for use;

[0...

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Abstract

The process of screening nematocidal Bacillus thuringiensis strain includes the steps of soaking bioassay, culture medium bioassay, protein crystal bioassay, etc. The process adopts easy-to-obtain defect type colibacillus JM109, and has short screening period, low cost, high screening efficiency. The screened nematocidal Bacillus thuringiensis strain IPS has a 24 hr LC50 of 1.008 mcg/ml.

Description

[0001] Technical field The present invention relates to a method for screening nematicidal strains in microorganisms, especially a method for screening nematicidal highly virulent strains in Bacillus thuringiensis. Background technique [0002] Bacillus thuringiensis (Bt for short) is a very important entomopathogenic bacterium, which is widely distributed all over the world. Thousands of strains have been isolated, purified and tested for their insecticidal activity. It has been found that Bt has important effects on many Agricultural pests including Lepidoptera, Coleoptera, Diptera, Hymenoptera, mites and nematodes have high specific insecticidal activity, and are very safe against humans, animals and non-target insects, such as natural enemy insects, so they have been It is widely used to control many important agricultural pests and is by far the most successful biopesticide. However, there have been few reports on nematodecidal Bt strains for a long time. [0003] Since ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 关雄余洁黄必旺黄志鹏黄天培张灵玲沙莉
Owner FUJIAN AGRI & FORESTRY UNIV
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