The invention provides a blueberry
seedling method and application thereof. The method comprises the following steps: selection and treatment of plants and explants, induction culture of axillary buds in an improved culture medium,
zeatin (ZT) decreasing subculture, and further cultivation of subculture seedlings into commercial seedlings. According to the method provided by the invention, the basic culture medium prepared from the improved MS and WPM culture media is adopted, so that explants germinate quickly, the subculture period is short, seedlings grow well, branching is reasonable, and buds are thick and strong. The induction
budding rate of the blueberries is increased to 92.5%, and the
multiplication rate multiple after five times of subculture reaches 404.8 times. When commercial seedlings are cultured for 25 days by adopting a one-step
seedling method, the rooting rate reaches 98.2%, the
seedling culture time is averagely shortened by 19.4 days, and root systems are dispersed and uniform. According to the scheme, the seedling raising efficiency, the seedling quality and the
plant growth vigor after planting are effectively improved, and the variation risk is reduced. Various
root system problems which seriously affect survival, growth and yield after planting are fundamentally solved.