242 results about "Method screening" patented technology

By exposing somatic cells to a component derived from ES cells to act on somatic cells and detecting the activity thereof, the reprogramming agent can be screened. Further, by exposing somatic cells to a component including a reprogramming agent or an isolated reprogramming agent, the somatic cells can be reprogrammed. Furthermore, according to the present invention, since the tetraploid cells have proliferating capability and pluripotency, such cells can be differentiated and the cells, tissues or organs, which can be used for transplantation, can be produced.

Provided are a system and methods for selectively inducing expansion of a population of T cells in the absence of exogenous growth factors, such as lymphokines, and accessory cells for research purposes. The cell based expansion system and methods permit the long-term growth of CTLs, preferably human CTLs. In addition, T cell proliferation can be induced without the need for antigen, thus providing an expanded T cell population that is polyclonal with respect to antigen reactivity. Further provided are methods for using the system and methods to screen and identify antigens related to specific diseases or conditions, tumors, autoimmune disorders, or an infectious disease or pathogen, and to identify target molecule for research purposes, or for developing a vaccine based thereon.

The invention discloses a screening method of colorectal cancer treatment prognosis biomarkers. The screening method includes the following steps of: (1) making a primary search strategy for screening the biomarkers; (2) making a literature adopting and exclusion criterion; (3) analyzing data and primarily screening the biomarkers; (4) making evident grades; (5) making a high quality evident evaluation criterion; (6) performing grading retrieval and quality evaluation on biomarker clinic evident; (7) performing data analyzing and statistics on the biomarker clinic evident; (8) screening the prognosis biomarkers. The invention further provides the colorectal cancer treatment prognosis biomarkers obtained through screening according to the screening method, and the prognosis biomarkers include KRAS, TYMS, TYMS, EGFR, UGT1A1*28, DPYD, PIK3CA, ERCC1, PTEN, VEGFR, BRAF, GST P1, XRCC1, MTHFR, MSI and the like.

The invention belongs to the field of biological medicines, and relates to a method for screening HRDs disease-causing mutation and a gene chip hybridization probe designing method involved in the same. The method for screening the HRDs disease-causing mutation comprises the steps of (1) establishing an HRDs genetic resource repository; (2) designing and synthesizing a gene chip hybridization probe of an HRDs disease-causing gene, and integrating the gene chip hybridization probe onto a gene chip; (3) capturing a target area by utilizing the prepared gene chip and executing the depth sequencing; (4) analyzing the sequencing data on the aspect of bioinformatics, and screening the candidate disease-causing gene; (5) functionally predicting a newly-discovered splicing gene mutation site. By establishing the high-efficient HRDs target gene capturing technology, adopting the depth sequencing as a means and confirming the efficiency of the HRDs capturing chip, a high-efficient credible biological information analysis model is established.

The invention discloses a preparation of efficient phenol-degrading halophilic bacteria and an application thereof, belonging to the technical field of treatment of waste water and sewage. In the invention, halophilic bacteria in the sewage or sludge are taken as a bacteria source; a gradient domestication method is adopted to screen efficient phenol-degrading halophilic bacteria which have strong degradation capability, can adapt to higher salt concentration and can grow well by taking phenol as the sole carbon source in the phenol-containing waste water with the salt content of 5-6% and thephenol concentration of 100-500mg/L; under the environment of high salt and high phenol, the phenol-degrading performance of the mycelium has obvious advantages over that of the traditional activatedsludge method. The strain can be applied to phenol-degrading treatment of waste water to determine the optimal water power retention time and the optimal bacteria input amount and batch processing time, and the operational conditions can be optimized through orthogonal experiments. The screening of the efficient phenol-degrading halophilic bacteria has high application value, and can provide a reference for the removal of phenol in the oil extraction waste water and the industrial treatment of the phenol-containing wastewater with high content of salt in petrochemistry industry.

The invention discloses a method for modifying recycled aggregate based on aerobic basophilic mixed bacterium mineralization deposition. The method comprises I, screening aerobic basophilic mixed bacteria and II, modifying recycled aggregate. Through a bacterial screening method, aerobic basophilic mixed bacteria having good practicability to the survival environment are screened. In the environment with enough calcium sources, oxygen and water, the mixed bacteria realize mineralization deposition of a large amount of calcium carbonate precipitates through metabolism. Through the excellent mineralization deposition capacity of the mixed bacteria, cracks and holes on the surface of recycled aggregate are repaired. In modification of the recycled aggregate, the desired mixed bacteria are screened and then are cultured, then the bacterium solution is adsorbed by the surface of recycled aggregate through a vacuum absorption method, and a calcium lactate solution is sprayed to the recycled aggregate to cure the recycled aggregate so that the surface defects of the recycled coarse aggregate are repaired. The method can effectively improve the physical and mechanical properties of the recycled coarse aggregate.

The invention discloses a method for screening denitrifying bacterium. The method comprises the following steps: (1) performing accumulation culture process on aerobic activated sludge by using ammonia-containing sewage; (2) separating and purifying denitrifying bacterium from accumulated flora; (3) acclimatizing the growing ability of the purified denitrifying bacterium with the water containingtarget pollutant; and (4) acclimatizing the denitrification ability of the acclimatized denitrifying bacterium under different dissolved oxygen conditions. When the denitrifying bacterium screened byusing the method disclosed by the invention is applied to denitrification treatment of sewage, the denitrifying bacterium not only can overcome the shortcomings of the traditional biologic denitrification technology, but also can be replenished into the impacted sewage treatment system as bioremediation agent so as to quickly recover the treating capability of the system and provide the conditionof synchronous nitration and denitrification.

The invention relates to a method which uses microsatellite marking technology for identifying the animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects microsatellite marks with specific property among the types and universal property in the type, designs specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.

The invention provides a method for preparing iminodiacetic acid by catalyzing iminodiacetonitrile with microbes, which comprises the following steps: using bacterial strains which produce nitrilase to obtain the nitrilase through enzyme production cultivation; and taking the nitrilase as a biological catalyst to biologically catalyze the iminodiacetonitrile to prepare the iminodiacetic acid. The invention also relates to a screening method of the microbes which produce the nitrilase and the bacterial strains with nitrilase activity obtained by the screening method. The invention provides the basis for producing the iminodiacetic acid by biologically catalyzing the iminodiacetonitrile, thereby having important application prospect.

The invention discloses a screening and construction method for a composite microbial system for rapidly degrading rice straw and a composite microbial system, which belong to the technical field of microbial screening methods. The method comprises the following steps of: (1) constructing a strain pond; (2) preparing a solid culture medium; and (3) screening and constructing the composite microbial system. The composite microbial system screened by the method comprises bacteria and fungi. The method and the composite microbial system have the advantages that: the composite microbial system is obtained by screening under the condition of the solid culture medium, comprises the bacteria and the fungi and has better decomposition effect; the composite microbial system RSS-4 is screened under the condition of unsterilized solid culture medium; the screened composite microbial system has better decomposition effect at the intermediate temperature, namely the temperature of 22 DEG C; and the defect that the composite strain can only decompose at the temperature of 28 DEG C existing in the prior art is effectively overcome.

The invention discloses a quick selecting predicting method of organic ER affinity force based on acceptor combine mode, which builds QSAR judge model and predict model according to the acceptor combine modes as prior ER affinity force data pollutant, acceptor combine energy, hydrogen bond function mode, acceptor function point reach hardness or the like, and the affinity index, to be applied into the ER affinity force judgment and predication of the compound with unknown affinity force. The invention can select and predict the compounds with different structures in wide active index ranges, to predict the suspected EDCs and hormone acceptor affinity force, with low cost, simple operation, saved time, and high predict ability as 80%, and significant support on the environment management and ecology risk evaluation.

The invention discloses a screening and domesticating method of a composite microbial system for low-temperature degradation of straws. The screening and domesticating method comprises the steps of acquiring a natural material sample rich in cellulose in an alpine area in which the annual average temperature is -0.6-8 DEG C, carrying out enrichment culture on a cellulose degradation microbial system, then carrying out restrictive subculture preliminary screening, low-temperature gradient induced domestication and cellulase enzyme activity re-screening, and then carrying out low-temperature multi-generation restrictive carbon source subculture domestication to obtain the composite microbial system for low-temperature degradation of straws. The screening and domesticating method can be used for screening and domesticating the composite microbial system for high-efficiency degradation of straws at low temperature and has an important meaning in field returning and utilization of straw resources in the northern area.

The invention provides a method for breeding a high-ammonia-nitrogen and high pH resistant species of a prawn, which can solve the problems of degraded quality, poor stress resistance and low breeding survival rate of prawn breeding existing in the prior art. Specifically, the new species with high stress resistance is screened by manually breeding the yellow sea No.1 fenneropenaeuschinensis with favorable growth characters as a basic group and adopting a combined breeding method of group breeding and family breeding through a high-ammonia-nitrogen and high pH stress test. The new high-stress resistance species screened by using the method is capable of aggregating and growing, has favorable characters of stress resistance and improves the high-ammonia-nitrogen and high pH resistance by 30 percent.

The invention discloses a method and apparatus for processing cervical cytological image features. The cervical cytology image data is compressed to different resolutions and input into a region nomination network to obtain a region nomination frame and a cervical cytology image characteristic map. In the cervical cytology image feature map, the feature corresponding to the region nomination box is selected as input, and the pool feature map is obtained through the grid pool layer. The classification probability of the region and the deviation between the prediction box and the nomination boxare obtained by inputting the pooled characteristic map into the classification network. The loss function is obtained by calculating the loss of the regional nomination network and the loss of the classification network. The convergent Faster RCNN model is obtained by back propagation optimization. Finally, a non-maximum suppression method is used to screen Faster RCNN trained by images with different resolutions to obtain a final prediction frame. The invention can effectively improve the efficiency and accuracy of screening unconventional cells in cervical cytology images by doctors.

The invention discloses a method for screening new internal reference molecules suitable for cervical tissue micro RNA real-time fluorescence quantitative PCR research. The method comprises the following technical steps: screening human Micro RNA probes by using a Micro RNA chip, and selecting Micro RNAs with constant normal tissue expression and high expression abundance of cervical cancer and cervix as candidate internal references; selecting a normal cervical epithelial tissue and a cancerous cervical tissue as clinical cervical tissue specimens respectively, and verifying the specimens by using fluorescence quantitative PCR; and analyzing data by using two special internal reference analysis software comprising geNorm software and Norm Finder so as to discover the internal reference molecules suitable for cervical tissue Micro RNA research. The internal reference molecules screened and verified by using the method of the invention can avoid possible differences of different cervical tissue specimens on the yield, quality and reverse transcription efficiency of the RNA, well correct and standardize the data for real-time fluorescence quantitative PCR detection, and improve the accuracy and the reliability of the search.

Objective methods for detecting and diagnosing intestinal-type gastric cancers are described herein. Also described is method for predicting the presence or absence of lymph node metastasis (i.e., identifying the metastatic phenotype). In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between lymph node positive tumors and lymph node negative tumors. The predictive score calculated acts as diagnostic indicator that can objectively indicate whether a sample tissue has the metastatic phenotype. The present invention further provides methods of diagnosing intestinal-type gastric cancer in a subject, methods of screening for therapeutic agents useful in the treatment of intestinal-type gastric cancer, methods of treating intestinal-type gastric cancer and method of vaccinating a subject against intestinal-type gastric cancer.

Screening conditions of SNP sites in paternity identification detection are optimized, and a multi-PCR primer group formed by SNP genetic marker sites is built according to the screening conditions. Human paternity identification is carried out by adopting the primer group, so that the defects of non-invasive paternity identification in early pregnancy can be overcome, the accuracy is high, and the multi-PCR primer group can be applied to judicial expertise and forensic medicine study.

The present invention belongs to the field of plant transgene technology, and is especially agrobacterium mediating method of cultivating transgenic plant of sycamore as one garden tree. The agrobacterium mediating method includes the steps of genetic transformation, PCR detection and Southern hybridization detection, and features that through agrobacterium mediation to transduce GUS reporter gene to acceptor sycamore, adding kanamycin as selecting agent to the basic culture medium, screening the transformed acceptor cell, PCR detection, GUS gene detection, Southern hybridization and other steps, transgenic plant of sycamore is obtained. The present invention provides technological platform for the genetic improvement of tree, especially sycamore.

The invention relates to an SNP (single-nucleotide polymorphism) locus related to heat resistance of Paralichthys olivaceus. The SNP locus is 47th/48th site (the base is AA or A/DEL), 65th site (the base is T or C), 185th site (the base is C or T), 260th site (the base is C or T), 305th site (the base is T or A), 600th site (the base is T or C) or 815th site (the base is C or T) of HSP70 gene with the nucleic acid sequence of SEQ ID NO:1. The SNP locus is used for screening the heat-resistant individual/family of Paralichthys olivaceus. The heat-resistant family is screened by a molecular marker process by using the heat shock protein gene HSP70; the hereditary characters of all the families can be evaluated on the molecular level, and the mutant site of the genome can be clearly identified; and the relevance between the mutant site and heat resistance is analyzed by combination of the heating experiment to perform reasonable judgment on the heat-resistant molecular mechanism. Compared with the traditional breeding method, the method provided by the invention has the advantages of higher purposiveness and lower cost, and can obviously shorten the breeding time.

The invention relates to a method for constructing a composite bacteria system for decaying maize straws, which belongs to the technical field of a microorganism screening method. The method for constructing the composite bacteria system for decaying the maize straws comprises the following steps of: (1) performing enrichment culture on bacteria; (2) preparing a solid culture medium; (3) performing inducing culture of nutrient limitation; (4) performing inducing culture of gradient temperature reduction; and (5) performing successive transfer culture to obtain the composite bacteria system CSS-1 for decaying the maize straws. The composite bacteria system screened by the method comprises bacteria, fungus and actinomycetes. The method has the advantages that: the composite bacteria system which comprises the bacteria, the fungus and the actinomycetes is obtained by screening under the condition of the solid culture medium; and the decaying effect is good.