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Tissue culture propagation method of Gynura formosana

A technology of tissue culture and Baifengcai, which is applied in the field of biological culture and reproduction, can solve the problems of tissue culture that have not been reported yet, and achieve the effects of neat seedling emergence, rapid seedling emergence and high reproductive frequency

Inactive Publication Date: 2014-03-19
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Baifengcai is mainly propagated by cuttings, and tissue culture has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for tissue culture and propagation of baifengcai, comprising the following steps:

[0035] A. Take the stems of Gynura formosana Kitam. as explants, rinse with running water for 3-5 minutes, soak in detergent, shake at 100-150 for 15-20 minutes at room temperature, and then rinse with water 15-30 minutes, then place the washed explants in 0.1% mercuric chloride, immerse and sterilize for 10 minutes, rinse with sterile deionized water 6-8 times, and set aside;

[0036] A. Selection and sterilization of explants: use the stems of Baifengcai as explants, soak them in detergent, shake on a shaker at 100-150 for 15-20 minutes, then rinse with water for 15-30 minutes , then place the washed explants in 0.1% mercuric chloride, soak and sterilize for 9-14 minutes, rinse with sterile deionized water for 6-8 times, and set aside;

[0037]B. Explant inoculation: On the ultra-clean workbench, cut the explants through step A into small pieces of 0.5-0.8 cm long, and inocul...

Embodiment 2

[0046] A method for tissue culture and propagation of baifengcai, comprising the following steps:

[0047] A. Take the stems of Gynura formosana Kitam. as explants, rinse with running water for 3-5 minutes, soak in detergent, shake at 100-150 for 10-15 minutes at room temperature, and then rinse with water After 20-30 minutes, place the washed explants in 0.1% mercuric chloride, immerse and sterilize for 11 minutes, rinse 6-8 times with sterile deionized water, and finally dry the water with sterile filter paper for later use ;

[0048] B. Inoculation of explants: On the ultra-clean workbench, cut the explants through step A into small pieces of 0.3-0.5 cm long, and inoculate them on the callus induction medium. The medium is: MS+ L-proline 1.0 mg / L+2,4-D 0.8 mg / L +NAA 0.4mg / L, add 0.7% agar, 3% sucrose and 0.5% activated carbon to the culture medium, cultivate for about 30-35 Days later, it can be seen that the callus tissue is swelled and formed at the cut of the stem;

...

Embodiment 3

[0058] A method for tissue culture and propagation of baifengcai, comprising the following steps:

[0059] A. Take the stems of Gynura formosana Kitam. as explants, rinse with running water for 3-5 minutes, soak in detergent, shake at 100-150 for 10-15 minutes at room temperature, and then rinse with water After 20-30 minutes, place the washed explants in 0.15% mercuric chloride, immerse and sterilize for 10 minutes, rinse with sterile deionized water for 6-8 times, and finally dry the water with sterile filter paper for later use ;

[0060] B. Inoculation of explants: On the ultra-clean workbench, cut the explants through step A into small pieces of 0.3-0.5 cm long, and inoculate them on the callus induction medium. The medium is: MS+ L-proline 2.0 mg / L+2,4-D 1.0mg / L +NAA 0.5mg / L, add 0.8% agar, 3% sucrose and 0.2% activated carbon to the culture medium, cultivate for about 30-35 Days later, it can be seen that the callus tissue is swelled and formed at the cut of the stem;...

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Abstract

The invention provides a tissue culture propagation method of Gynura formosana. The method comprises the following steps of: with the stem of Gynura formosana as an explant, carrying out sterilizing, grafting and inducing operations to form callus; then, forming an integral plant seedling through proliferation of callus, bud induction and root induction; transplanting the integral plant seedling to a culture medium so that the integral plant seedling grows up inti normal Gynura formosana plant, wherein the basic culture medium is based on MS culture medium and assisted with components such as L-proline, 6-benzyladenine, 2, 4-dichlorphenoxyacetic acid, naphthylacetic acid, cane sugar, active carbon and the like. The method provided by the invention overcomes the problem of long culturing period and low propagation coefficient of Gynura formosana by using tissue culture, and industrial quick seedling can be performed, so that the method meets demand on Gynura formosana in market.

Description

technical field [0001] The invention relates to the cultivation and propagation of organisms, in particular to a tissue culture and propagation method of baifengcai. Background technique [0002] Baifengcai (Gynura formosana Kitam.) is a perennial medicinal and edible plant of Compositae (Compositae), which is native to Taiwan. "Baifengcai" is also known as Baidang, and its common name is Hepatitis Grass. Baifengcai Baifengcai is rich in nutrients. Every 100 grams contains 2 grams of protein, 573.3 mg of vitamin A, 24.5 mg of vitamin C, and rich trace elements, including 380 mg of potassium, 82 mg of calcium, 42 mg of phosphorus, and 0.4 mg of zinc. Because of its high zinc content, it is also known as "smart vegetable". It is an emerging vegetable variety. The tender stems and leaves are edible parts. In recent years, it has become a rookie in the vegetable, health care and medical markets in major cities in China, and is welcomed by consumers. The whole herb or stems...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 穆红梅张秀省杜秀菊
Owner LIAOCHENG UNIV
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