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Tissue culture propagation method of Gynura formosana

A technology of tissue culture and baifengcai, which is applied in the field of biological culture and reproduction, can solve the problems of tissue culture that have not been reported yet, and achieve the effects of neat emergence, high frequency of reproduction, and rapid emergence

Inactive Publication Date: 2013-08-07
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Baifengcai is mainly propagated by cuttings, and tissue culture has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for tissue culture and propagation of Phyllostachys pubescens, comprising the following steps:

[0035] A. Take the stem section of Gynura formosana Kitam. as explants, rinse with running water for 3-5 minutes, soak in detergent, shake at room temperature at 100-150 rpm for 15-20 minutes, and then rinse with water 15-30 minutes, then put the washed explants in 0.1% mercuric chloride, soak and sterilize for 10 minutes, rinse with sterile deionized water for 6-8 times, and set aside;

[0036] A. Selection and sterilization of explants: take the stems of white phoenix as explants, soak them in detergent, shake the shaker at 100-150 rpm for 15-20 minutes, and then rinse with water for 15-30 minutes , and then place the washed explants in 0.1% mercuric chloride, soak and sterilize for 9-14 minutes, rinse with sterile deionized water for 6-8 times, and set aside;

[0037]B. Inoculation of explants: on the ultra-clean workbench, the explants after step A are cut into...

Embodiment 2

[0046] A method for tissue culture and propagation of Phyllostachys pubescens, comprising the following steps:

[0047] A. Take the stem segment of Gynura formosana Kitam. as explants, rinse with running water for 3-5 minutes, soak in detergent, shake at room temperature at 100-150 rpm for 10-15 minutes, and then rinse with water 20-30 minutes, then put the washed explants in 0.1% mercuric chloride, soak and sterilize for 11 minutes, rinse with sterile deionized water for 6-8 times, and finally use sterile filter paper to absorb water for later use ;

[0048] B, explant inoculation: on the ultra-clean workbench, cut the explant after step A into small pieces with a length of 0.3-0.5 cm, and inoculate it on the callus induction medium, the medium is: MS+ L-proline 1.0 mg / L+2,4-D 0.8 mg / L +NAA 0.4mg / L, add 0.7% agar, 3% sucrose and 0.5% activated carbon to the medium, and culture about 30-35 Days later, it can be seen that callus swelled at the incision of the stem;

[0049] ...

Embodiment 3

[0058] A method for tissue culture and propagation of Phyllostachys pubescens, comprising the following steps:

[0059] A. Take the stem segment of Gynura formosana Kitam. as explants, rinse with running water for 3-5 minutes, soak in detergent, shake at room temperature at 100-150 rpm for 10-15 minutes, and then rinse with water 20-30 minutes, then put the washed explants in 0.15% mercuric chloride, soak and sterilize for 10 minutes, rinse with sterile deionized water for 6-8 times, and finally use sterile filter paper to absorb water for later use ;

[0060] B, explant inoculation: on the ultra-clean workbench, cut the explant after step A into small pieces with a length of 0.3-0.5 cm, and inoculate it on the callus induction medium, the medium is: MS+ L-proline 2.0 mg / L + 2,4-D 1.0 mg / L + NAA 0.5 mg / L, add 0.8% agar, 3% sucrose and 0.2% activated carbon to the medium, and culture for about 30-35 Days later, it can be seen that callus swelled at the incision of the stem; ...

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Abstract

The invention provides a tissue culture propagation method of Gynura formosana. The method comprises the following steps of: with the stem of Gynura formosana as an explant, carrying out sterilizing, grafting and inducing operations to form callus; then, forming an integral plant seedling through proliferation of callus, bud induction and root induction; transplanting the integral plant seedling to a culture medium so that the integral plant seedling grows up inti normal Gynura formosana plant, wherein the basic culture medium is based on MS culture medium and assisted with components such as L-proline, 6-benzyladenine, 2, 4-dichlorphenoxyacetic acid, naphthylacetic acid, cane sugar, active carbon and the like. The method provided by the invention overcomes the problem of long culturing period and low propagation coefficient of Gynura formosana by using tissue culture, and industrial quick seedling can be performed, so that the method meets demand on Gynura formosana in market.

Description

technical field [0001] The present invention relates to the cultivation and reproduction of organisms, in particular to a method for tissue culture and reproduction of Phyllostachys pubescens. Background technique [0002] Gynura formosana Kitam. (Gynura formosana Kitam.) is a perennial medicinal and edible plant of the Compositae family, native to Taiwan. "Bai Fengcai" is also known as Bai Zhan, and the common name is Hepatitis Grass. Cabbage Cabbage is rich in nutrients, each 100 grams contains 2 grams of protein, vitamin A 573.3RE, 24.5 mg of vitamin C, and rich trace elements, potassium 380 mg, calcium 82 mg, phosphorus 42 mg, and zinc 0.4 mg. Because of its high zinc content, it is also known as "smart vegetable". It is an emerging vegetable variety. The tender stems and leaves are edible parts. In recent years, it has become a rookie in the vegetable, health care and medical markets in major cities in China, and is welcomed by consumers. The whole plant or stems ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 穆红梅张秀省杜秀菊
Owner LIAOCHENG UNIV
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