A kind of tissue culture method of aseptic vaccine

A technology for tissue culture and sterile seedlings, applied in the field of plant tissue culture, can solve problems such as difficulty in reproduction, difficulty in obtaining plant seedlings, etc., and achieve the effects of overcoming difficulty in reproduction, a simple and easy tissue culture method, and a high plant survival rate.

Active Publication Date: 2022-03-08
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is easy to operate, easy to operate, and high in output, and overcomes the problems of difficult propagation in aquariums and difficulty in obtaining plant seedlings

Method used

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  • A kind of tissue culture method of aseptic vaccine
  • A kind of tissue culture method of aseptic vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1: Prepare the primary medium and proliferation medium

[0027] 1. The primary culture medium and proliferation medium prepared in this example are MS medium, and the formula is as follows: mg / L

[0028]

[0029] 2. Preparation of primary culture medium

[0030] Add 7g of agar, 30g of sucrose, 0.5mg of 6-aminopurine (6BA), and 0.1mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; The medium was divided into 150ml tissue culture bottles, 30ml in each bottle.

[0031] 3. Preparation of proliferation medium

[0032] Add 7g of agar, 30g of sucrose, 0.5mg of kinetin (KT), and 0.1mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Base fractions were packed into 150ml tissue culture bottles, 30ml per bottle.

[0033] Step 2: Cleaning and disinfection of e...

Embodiment 2

[0043] Step 1: Prepare the primary medium and proliferation medium

[0044] 1. The primary medium and proliferation medium prepared in this example are MS medium, and the formula is the same as that shown in Example 1.

[0045] 2. Preparation of primary culture medium

[0046] Add 7g of agar, 30g of sucrose, 1mg of 6-aminopurine (6BA) and 0.3mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Base fractions were packed into 150ml tissue culture bottles, 30ml per bottle.

[0047] 3. Preparation of proliferation medium

[0048] Add 7g of agar, 30g of sucrose, 1.25mg of kinetin (KT), and 0.3mg of naphthaleneacetic acid (NAA) to 1L of the above-mentioned MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Base fractions were packed into 150ml tissue culture bottles, 30ml per bottle.

[0049] Step 2: Cleaning and disinfection of exp...

Embodiment 3

[0059] Step 1: Prepare the primary medium and proliferation medium

[0060] 1. The primary medium and proliferation medium prepared in this example are MS medium, and the formula is the same as that shown in Example 1.

[0061] 2. Preparation of primary culture medium

[0062] Add 7g of agar, 30g of sucrose, 1.5mg of 6-aminopurine (6BA), and 0.5mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; The medium was divided into 150ml tissue culture bottles, 30ml in each bottle.

[0063] 3. Preparation of proliferation medium

[0064] Add 7g of agar, 30g of sucrose, 2mg of kinetin (KT), and 0.5mg of naphthaleneacetic acid (NAA) to 1L of the above MS medium to prepare a solid medium, and sterilize it at 98kPa and 115-125°C for 20min before use; Divide into 150ml tissue culture bottles, 30ml per bottle.

[0065] Step 2: Cleaning and disinfection of explants

[0066] Purchase the a...

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Abstract

The invention relates to a method for tissue culture of aseptic seedlings of Glycyrrhizae, comprising the following steps: (1) preparing primary culture medium and proliferation medium, and sterilizing them for use; (2) cleaning and disinfecting explants; (3) Absorb the surface moisture of the sterilized explants with sterilized paper, transfer them into the primary culture medium, then pour 30-60ml of cooled sterile water into the tissue culture bottle, and sink the explants in sterile water. In water, place it in an incubator to induce culture for 4 weeks; (4) separate the explants and the sprouts produced in the primary culture medium, transfer them to the proliferation medium together, pour 30-60ml of cooled sterile water, and submerge New explants and new shoots were proliferated and cultured for 4 weeks; (5) the plants with complete root systems were taken out, rinsed with clean water, and then transplanted into simulated natural water bodies for rooting culture. The method can obtain a large number of new plants without being affected by seasons, temperatures and regions, the rooting rate of the new plants reaches 100%, the survival rate is high, and the aseptic propagation system of the algae can be quickly established.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of aseptic seedlings of Pleurotus spp. Background technique [0002] Grass algae, also known as water screen, is a more commonly used ornamental aquatic plant. Grass algae is a submerged herbaceous plant, which belongs to the family Hydrochardaceae with Hydra verticillium, and is a monocotyledonous plant. The leaf color of the algae is beautiful, and it is an important arrangement material for submerged plants in the aquarium, and it is also a good greening material in the garden waterscape. Grassia has thin white petals and is suitable for planting in the foreground area of ​​a flat aquarium. However, as Grass is a positive grass, it is not suitable for light, CO 2 There are strict requirements on water quality and water depth. A little carelessness may lead to plant death and leaf fall. Therefore, it is extremely difficult to plant. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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