Tissue culture method for cultivating amaryllis vittata by utilizing bulbs

Abstract

The invention relates to a tissue culture method for cultivating amaryllis vittata by utilizing bulbs. The tissue culture method comprises the following steps: selecting and disinfecting explants; performing induction culture; performing subculture multiplication culture; rooting; transplanting. According to the invention, small bulbs of amaryllis vittata are taken as explants, so that the reduction of contamination rate is benefited and the induction rate is increased; cluster buds can be induced without callus stage; the cluster buds can be directly induced so as to acquire bulbs; the induction time is shortened; the buds can be added for multiplication culture; the multiplied test bulbs are rooted; one-step bulb forming of amaryllis vittata bulbs can be realized according to the invention; the time of tissue culture is saved; the operation steps are reduced; the excellent natures of original bulbs are kept; an effective method is supplied for industrial efficient cultivation of amaryllis vittata; a technical support is supplied for the industrial development of amaryllis vittata.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a tissue culture method for breeding Hippeastrum by using bulbs. Background technique [0002] Hippeastrum is the general term for the hybrid species of the genus Amaryllis in the family Amaryllidaceae. It has obvious advantages such as large and colorful flowers and rich shapes, and has high ornamental and economic value. Some varieties have been introduced into our country, and the market acceptance is high. Hippeastrum has become one of the emerging flower varieties. However, the natural reproduction rate of Hippeastrum bulbs is very low, and most of the varieties and hybrids hardly produce seed balls. [0003] Although there is also the production of Hippeastrum bulbs in Asia, the production facilities are relatively backward. Domestic Hippeastrum varieties are relatively single, and the bulbs are produced less. The price greatly limits the development of this b...

AI Technical Summary

Problems solved by technology

[0007] At present, there are many choices of explants for tissue culture of Hippeastrum, including large bulbs, leaves, scapes, pedicels, etc., but only when the double scales in the bulb are used as explants, clustered buds can be induced, and other explants can induce All can only get callus, and when the big bulb is used as explant, the disinfection efficiency is low, and the pollution rate can reach 90%. Directly carry out rooting culture, or carry out strong ball culture again, and finally transplant, the survival rate of transplanting is 70-90%

[0008] It can be seen that the existing Hippeastrum tissue culture explants have low disinfection efficiency, low proliferation coefficient, limited transplant survival rate, strong bulb culture is required in the proliferation process, and the operation steps are cumbersome, etc.

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