The invention discloses a preparation method of dedifferentiated
saussurea involucrata cells and anti-
wrinkle tightening application of the dedifferentiated
saussurea involucrata cells. The preparation method comprises the following specific steps: S1, transferring root tips of aseptic seedlings to an induction culture medium, and proliferating to form
cell clusters; culturing the
cell cluster in a culture dish containing a proliferation culture medium to obtain primary
saussurea involucrata stem cells; s2, transferring the obtained primary
saussurea involucrata stem cells into a liquid MS culture medium for
suspension culture, and meanwhile, changing the illumination condition, and culturing to obtain the
saussurea involucrata stem cells of which the total flavone content and the
chlorogenic acid content are obviously increased. According to the method, large-scale production of
saussurea involucrata stem cells is realized through a
plant cell culture technology, the problem of scarcity of natural resources is solved, and rare and sustainable raw materials are realized; according to the method, unique culture conditions and
inducer combination are adopted, so that the activity and the
active ingredient content of the saussurea involucrata stem cells are improved, and
process innovation is realized; meanwhile, the saussurea involucrata stem cells prepared by the method provide a safe
raw material with anti-
wrinkle and firming effects for the
skin care product industry.