Quality walnut tissue culture quick propagation method

A chestnut group, rapid technology, applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of variety degradation, fewer scions of excellent varieties, chestnut seed selection, and delayed breeding work.

Inactive Publication Date: 2005-07-06
孙立新
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Problems solved by technology

[0002] For many years, the chestnut seed selection and breeding work has lagged behind, coupled with the lax regional planting system, there are mixed varieties in the chestnut industry, the planting rate of superior varieties is not high, the yield is low, the result is late, the yield is unstable, and the rate of first-class fruit is low. Problems, it is very necessary to use high technology to rapidly multiply the excellent chestnut varieties to improve the existing chestnut varieties and improve the yield and quality of chestnut
At present, the method of grafting is mainly used for the propagation of excellent chestnut varieties in China. Due to the disadvantages of variety degradation, few scions of excellent varieties, and high cost in this method, the promotion speed of excellent chestnut varieties is limited.

Method used

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Embodiment Construction

[0012] The present invention is described in detail below in conjunction with embodiment.

[0013] The method for rapid multiplication of the excellent species of Chinese chestnut by tissue culture described in this example is suitable for industrial seedling cultivation of the excellent species of Chinese chestnut. The method is divided into two stages: the first stage is the seedling tissue culture stage, and the second stage is the nutrient cup seedling cultivation stage, wherein the seedling tissue culture stage is the key technology of the present invention. The specific operation has the following steps:

[0014] ① Selection of explants. The specific method is: select the stem section of the annual chestnut from the chestnut tree of the excellent species in early spring as the explant, because the growth activity of the stem section of the annual chestnut is relatively strong.

[0015] ② Selection of starting medium. The specific method is: according to the characteri...

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Abstract

The invention relates to a quality walnut tissue culture quick propagation method which comprises, appending right amount of iron ions and zinc ions into chestnut culture medium, adjusting nutrient content of culture medium, screening the most suitable culture medium for chestnut growth, selecting right explant for tissue culture at right stage, readjusting the culture medium formulation so as to grow shoot and root for the chestnut, transplanting it into warm house and planting into big nourishing cup equipped with nourishment base materials, finally planting and transplanting the nursery stock in the nourishment cup.

Description

Technical field: [0001] The invention relates to the technical field of fruit tree cultivation, in particular to a method for tissue culture and rapid propagation of an excellent chestnut species. Background technique: [0002] For many years, the chestnut seed selection and breeding work has lagged behind, coupled with the lax regional planting system, there are mixed varieties in the chestnut industry, the planting rate of superior varieties is not high, the yield is low, the result is late, the yield is unstable, and the rate of first-class fruit is low. The problem is that it is very necessary to use high-tech to rapidly multiply the excellent chestnut varieties to improve the existing chestnut varieties and improve the yield and quality of chestnut. At present, the method of grafting is mainly used for the propagation of excellent chestnut varieties in China. This method has the disadvantages of variety degradation, few scions of excellent varieties, and high cost, whic...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孙立新杨军李强王凤春吴桂军
Owner 孙立新
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