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Angelica tissue culture breeding method

A tissue culture and Qiang living technology, which is applied in the field of biological culture and reproduction, can solve the problem that wild Qiang living tissue culture has not yet been reported, and achieve the effects of fast reproduction speed, fast start-up and high proliferation frequency.

Inactive Publication Date: 2007-02-28
蒋舜媛 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The tissue culture of other alpine wild medicinal materials, such as Rhodiola rosea, has been reported in the literature, but the tissue culture of wild notopterygium has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A notopterygium tissue culture propagation method, comprising the following steps:

[0032] A. Take notopterygium incisum Ting ex H.T. Chang germinated root buds as explants, rinse with running water for 3-5 minutes, then sterilize with 75% alcohol for 50 seconds, rinse with sterile deionized water for 2-3 times, Then add 0.1% mercuric chloride, sterilize for 14 minutes, rinse with sterile deionized water twice, and finally dry the water with sterile filter paper for later use;

[0033] B. Inoculation of explants: On the ultra-clean workbench, cut the explants after step A into 0.1-0.3cm 3The small pieces of the callus were inoculated on the callus induction medium, the medium was: MS+KT2.0mg / L+2, 4-D 0.5mg / L+NAA 0.2mg / L, and 0.8% of Agar, 3% sucrose and 0.1% activated carbon, after culturing for about 35 days, it can be seen that the callus tissue is enlarged and formed at the cut of the root bud;

[0034] The ultra-clean workbench, model HS-1300, is produced by Suji...

Embodiment 2

[0043] A notopterygium tissue culture propagation method, comprising the following steps:

[0044] A. Take the root buds germinated from Notopterygium incisum Ting ex H.T.Chang as explants, rinse with running water for 3-5 minutes, then sterilize with 75% alcohol for 50 seconds, rinse with sterile deionized water for 3 times, and then add 0.15% mercury chloride, sterilized for 12 minutes, rinsed with sterile deionized water for 3 times, and finally blotted dry with sterile filter paper for later use;

[0045] B. Inoculation of explants: On the ultra-clean workbench, cut the explants after step A into 0.1-0.3cm 3 The small pieces were inoculated on the callus induction medium, the medium was: MS+KT1.0mg / L+2, 4-D 0.8mg / L+NAA 0.4mg / L, and 1.0% of Agar, 3% sucrose and 0.5% activated carbon, after 30 to 35 days of culture, callus will be formed at the incision of the root bud;

[0046] The ultra-clean workbench, model HS-1300, is produced by Suzhou Antai Air Technology Co., Ltd. ...

Embodiment 3

[0055] A notopterygium tissue culture propagation method, comprising the following steps:

[0056] A. Take the root buds germinated from Notopterygium incisum Ting ex H.T.Chang as explants, rinse with running water for 3 to 5 minutes, then sterilize with 75% alcohol for 45 seconds, rinse twice with sterile deionized water, and then add 0.2% mercury chloride, sterilized for 12 minutes, rinsed with sterile deionized water for 3 times, and finally blotted dry with sterile filter paper for later use;

[0057] B. Inoculation of explants: On the ultra-clean workbench, cut the explants after step A into 0.1-0.3cm 3 The small pieces were inoculated on the callus induction medium, the medium was: MS+KT0.2mg / L+2, 4-D 1.0mg / L+NAA 0.5mg / L, adding 0.8% agar, 3% sucrose and 0.2% gac, after 30-35 days of culture, callus will be formed at the cut of the root bud;

[0058] The MS is the abbreviation of conventional basic medium Murashige&Skoog 1962, KT is 6-furfuryl adenine, 2,4-D is 2,4-dic...

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PUM

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Abstract

The invention discloses an angelica tissue culturing and breeding method, which comprises the following steps: adopting angelica root as external growing bulk, sterilizing, grafting to induce callus, breeding, proceeding bud and root induction, forming entire plant sprout, planting the entire sprout in the substrate, adopting MS culture medium as base and 6-furfuryl adenine, 6-benzyl adenine, 2, 4-dichlorophenoxyacetic acid, fruitone, sucrose and active carbon as auxiliary compositions.

Description

Technical field: [0001] The invention relates to the cultivation and propagation of organisms, in particular to a method for culturing and propagating notopterygia tissue. Background technique: [0002] Notopterygium is a perennial wild medicinal material of Umbelliferae (Umbelliferae), a unique plant species in my country, and one of the most commonly used medicinal materials in Chinese and Tibetan Qiang medicine. Notopterygium is used as medicine with rhizomes and roots. It is pungent in taste and warm in nature. It has the functions of dispersing cold, dispelling rheumatism, and sharpening joints. Notopterygium incisum Ting ex H.T.Chang and N.forbesii Boissieu are the dried rhizomes of Notopterygium incisum Ting ex H.T.Chang and N.forbesii Boissieu according to the 2005 edition of the Pharmacopoeia of the People's Republic of China. And roots, with a long history of development, medicinal products include: silkworm Qiang, Zhujie Qiang, Tou Qiang, Tiao Qiang and Qiang Kin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 蒋舜媛陈学军马小军梁锦添
Owner 蒋舜媛
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