40results about How to "Good genetic traits" patented technology

The invention discloses a Japanese maple air layering propagation method which includes selecting a seed tree, selecting branches, air layering, forming new trees, and transplanting new trees. The method has the advantages that the branches, grown 2-3 years, on a growing Japanese maple are used for air layering, allowing the branches to grow on the seed tree in a nutritious manner for 65-72 days, and forming a root system at the ringing cut; the branch rooting rate is 80%, and the transplanting survival rate can reach more than 78%; the air layering propagation method can keep the excellent hereditary features of the seed Japanese maple; the method is simple in technology, convenient to operate, low in cost, high in survival rate and important for Japanese maple seedling fast propagation.

The invention discloses a method using tissue-subculture sprouts of oil-tea camellia (Camellia oleifera Abel) to raise seedlings by grafting, particularly a method using the tissue-subculture sprout of high-grade oil-tea camellia as a scion and using the germinated germ of oil-tea camellia seeds as a stock to culture a novel plant in a shed sealed by plastic film at 10 to 35 DEG C with the air humidity being 100% on the basis of the method of cleft grafting or slope grafting, wherein the stock seeds are stored at 4 to 5 DEG C by humid sand in an icehouse having high grafting capacity and seed storage capacity; and the grafting process is carried out day by day, and the germination acceleration is carried out group by group, accordingly, the survival rate reaches 75% to 95%. The method of the invention has the advantages of high reproductive rate, high seedling raising capacity, simple method, easy operation and low material consumption; and meanwhile, the offspring can maintain the good hereditary property of the stock plant, thus solving the problem that a large amount of improved branches and budlings are required for the existing method for the vegetative propagation of oil-tea camellia, such as cuttage propagation and seedling-raising by germ-stock grafting, and meeting the requirements of high-grade and high-quality clonal oil-tea camellia sprouts for the development of the oil-tea camellia industry in China.

The invention relates to an ecological-floating-bed-based water body ecological restoration method which comprises the following steps: 1) establishing an ecological floating bed, adding biological activated carbon into each growing basket, and suspending rope-shaped carbon fiber ecological grass on the bottom of the floating bed; 2) growing emerging plants in a first aquatic plant growing region, wherein the emerging plants are composed of more than two of Canna generalis, Thalia dealbata, Iris wilsonii, calamus, purple loosestrife, bamboo reed, reed, cyperus altrnlifolius and Pontederia corda; growing a floating leaf plant or submerged plant in a second aquatic plant growing region, wherein the floating leaf plant is Ludwigia stipulacea, and the submerged plant is watermilfoil; and 3) reaping: carrying out periodical reaping according to the growth condition of the aquatic plants. The multiple ornamental plants and plant species with higher synergic decontamination capacity are reasonably blended and grown to establish a stable floating bed phytocommunity, thereby enhancing the synergistic actions among organisms, enhancing the restorability of the ecological floating bed for the water ecological environment, effectively enhancing the purifying effect of the ecological floating bed, and having stabler purifying effects.

The invention discloses an erythrophloeum fordii cutting propagation method which includes the procedures of stock plant selection, cutting orchard building, stock plant management, cutting slip picking, cutting and post-cutting management. Excellent robust nursery plants free of pest diseases are selected as stock plants and are densely arranged in a rectangular piece to build a cutting orchard, top cutting is conducted to promote sprouting, twigs or semi wood is selected as cutting scions which are inserted in container cups filled with substrates, and cutting seedlings are cultured to obtain excellent robust erythrophloeum fordii cutting seedlings. The method solves the problem that erythrophloeum fordii is difficult in vegetative propagation, has the advantages of being free of geographic limitation, high in propagation speed, simple, easy to operate, high in survival rate and the like, maintains good hereditary character of the erythrophloeum fordii, has great significance on protection and expansion of propagation and planting of the erythrophloeum fordii and has good economical benefit, social benefit and ecological benefit.

The invention provides acid protease which is derived from microorganism sources and a preparation method thereof. The acid protease is characterized in that a. enzymology characteristics: the optimumpH is 2.5-3.5, the stable pH is 2.5-6.0, the optimum temperature is 40-50 DEG C, and the temperature stability ranges from 30 DEG C to 50 DEG C; b. with A. niger as an enzyme producing strain and wheat bran as main materials, the acid protease is prepared by solid fermentation process; the fermenting and enzyme producing capabilities are more than or equal to 47000u/g (based on dry yeast), the yield of liquid enzyme is more than or equal to 85% and that of solid enzyme is more than or equal to 80%, which all reach the food grade hygienic standards. The acid protease takes grain processing by-products as main raw materials and has high fermentation enzyme activity, high enzyme extraction purification yield and low production cost. The acid protease is suitable for serving as the preparation raw material or the additive of the products in such industries as tanning, medicine, brewing and feed.

The invention relates to the technical field of pueraria lobata planting, in particular to a planting method of high-yield pueraria lobata. The method comprises the following steps that during planting, front surfaces and reverse surfaces of pueraria lobata seedlings are distinguished, the smooth surfaces face upwards, and the surfaces with spores face downwards and are in contact with soil; during planting, the pueraria lobata seedlings are planted at the angle of 45 degrees, when many pueraria lobata seedlings grow, small and bad pueraria lobata seedlings need to be cut off, 1-3 good-qualityroots are reserved, and all the seedlings are evenly placed in pits separately; during planting, the head directions of the pueraria lobata seedlings are unified; during planting, soil covers the spores, and pueraria lobata heads are exposed; during planting, fine and loose soil is taken by hands to cover the pueraria lobata seedlings, then the soil is slightly pressed by hands, and then the pitsare filled with a large amount of soil; after planting, each plant is watered with 1-3 kg of rooting water immediately, and better growth and development of the pueraria lobata seedlings are guaranteed. According to the planting method, a traditional planting method of pueraria lobata is improved, the yield of pueraria lobata is increased, and the method is beneficial to planting and popularization of pueraria lobata.

The invention discloses a jackfruit branch topping and seedling growing method. Firstly, robust branches without diseases and pests are chosen from a jackfruit seed tree with excellent quality; secondly, girdling is conducted on the portion 15-20 cm away from the position where the branches are divaricated, 0.01%-0.06% ABT-1 solution is smeared on a girdling opening by one circle, the girdling position is bound with a wet matrix and wrapped in a thin film, and the two ends of the girdling position are bound through ropes; after branch topping is conducted for 5-25 days, diluted ethephon solution is added to a thin film bag; after branch topping is conducted for 40-50 days, a root system gradually grows from the girdling opening, and half-cutting treatment is conducted on the lower ends of the branches bound by the ropes; after half-cutting treatment is conducted for 20-30 days, the branches are cut off from the half-cut positions and subjected to heeling and transplanting. The jackfruit branch topping and seedling growing method is simple, operation is easy, the seedling growing period is short, the rooting percentage is high, the survival rate of transplanting is high, limitation of seasons is avoided, and the quality of grown seedlings is guaranteed at the same time.

An ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production includes 1, preserved spawn preparation; 2, protective agent preparation; and 3, preserving operation. The step of preserving operation includes (1), slicing agar of mother spawn source with mycelia into the bean sizes through an inoculating needle by sterile operation, placing the 4 to 6 agar slices into one sterile test tube, then adding the protective agent till covering the agar slices, sealing the sterile test tube, and marking through mark pens; (2), punching holes at the bottom, cover and side wall of plastic bottles, placing the sterile test tubes to the plastic bottles, then placing the plastic bottles the a ultra-low-temperature freezer, and decreasing the temperature to -80 to -150 DEG C at the speed of decreasing 1+-0.1 DEG C in every minute. By the aid of the ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production, the Pleurotus eryngii spawn can be maintained in extreme slow metabolism state for 3 to 5 years generally, and excellent genetic characteristics can be maintained constantly.

The invention belongs to the technical field of plant vegetative propagation, and particularly relates to a method for cultivating Chinese wistaria nursery stock. The method comprises the operation steps of overhead strip pressing, management after branch peeling, transplanting of nursery stock obtained after branch peeling and the like. Plants with flowering and fruiting seed trees and trunk diameter being 2.5 cm or above are selected, and robustly growing one-year-old lateral branches with the diameter being 0.5-1.0 cm and with no plant disease or insect pest are adopted as branch peeling branches, wherein the lateral branches germinate on the plant main branches. By adopting the overhead strip pressing method, branch peeling seedlings with developed root systems are obtained, the branchpeeling nursery stock is sheared and transplanted into a container for cultivation to obtain Chinese wistaria nursery stock. The cultivated Chinese wistaria nursery stock girdling opening rooting isfast, roots begin to grow at the 34 th day, the seedling forming stage is short, the nursery stock root system can be specially robust and strong, the transplanting survival rate can reach 91% or above, the transplanted nursery stock can grow rapidly, and after cultivation is performed for 3 months, the nursery stock can be sold. The method is simple and easy to implement, and low in cost, and theproblems that the Chinese wistaria cutting seedling rooting rate is low, the cutting seedling nursery stock is small, and time needed for flowing of the plants is long are solved. Technical supportscan be supplied for rapidly cultivating the Chinese wistaria nursery stock.

The invention relates to the technical field of eucalyptus cultivation, in particular to a tissue culture and rapid propagation method of a 1212 variety of Eucalyptus cloeziana F. Muell. According tothe tissue culture and rapid propagation method, excellent individual plants of Eucalyptus cloeziana F. Muell. excellent provenance are adopted, cut down and subjected to germination promoting, budlings are collected for inducing sterile buds, and regenerated plants are obtained through subculture, rooting culture and light-substrate seedlings of the sterile buds. The tissue culture and rapid propagation method of the 1212 variety of Eucalyptus cloeziana F. Muell. is low in operation difficulty, low in production cost, stable in growth of an aseptic propagation system, high in proliferation coefficient of subculture seedlings, good in repeatability and high in rooting rate of rooting seedlings, the produced tissue cultured seedlings are high in survival rate, stable in genetic traits and tidy in forest form, the seedlings can maintain the excellent genetic characteristics of excellent trees, large-area planting is suitable, and large-scale production and promotion are easy.

The invention discloses a hybrid method for improving the cold resistance of Ilex rotunda. The method includes the following steps of (1) pollen storage: taking a good Ilex cornuta varfortunei plant as a female parent from April to May, using a sterilized conical flask to collect male pollen, and filling the conical flask with nitrogen for storage after low temperature baking; (2) hybridization: activating the stored pollen firstly from March to April in the second year, when pollination needs to be conducted, using a sodium hypochlorite solution and a plant extract solution to spray Ilex flowers respectively, and then conducting pollination; (3) seed treatment: placing first-generation seeds into an ammoniation pool to conduct ammoniation treatment, and then conducting temperature changing treatment; (4) repeated hybridization: according to the way of step 2, conducting hybridization cultivation on the processed first-generation seeds and Ilex cornuta varfortunei to obtain second-generation seeds, conducting hybridization cultivation on the second-generation seeds and the Ilex cornuta varfortunei to obtain third-generation seeds, and then conducting intraspecific breeding cultivation on the third-generation seeds to obtain hybridized Ilex rotunda seeds; (5) cultivation: conducting greenhouse cultivation on the hybridized Ilex rotunda seeds after the treatment of step 3.

The invention relates to the technical field of agriculture plantation and especially relates a bending pruning seedling growing method for pearl guava. The method comprises following steps: a. bending pruning seedling selection; b. root promoting medium preparation; c. bending pruning: after girdling, wrapping a peeling mouth with the root-promoting media, and then wrapping the peeling mouth with rice straw and film, sawing off branches at the lower end of the peeling mouth after rooting; d. temporary planting: moving temporary planted seedlings out of the nursery after 1-2 years cultivation to obtain pearl guava planting stock. The invention provides a bending pruning seedling growing method for pearl guava, which has the advantages of convenient material obtaining, strong operability, high success rate of the bending pruning seedling growing, and keeping the excellent characters of the female parent, so as to achieve the purpose of rapidly obtaining the pearl guava seedlings for production.

The invention discloses a molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice. The method herein includes the steps of (1) using primers to mark first and tenth chromosome restoring genes SSR of four rice parents respectively, performing amplifying before electrophoresis to obtain restorer line genotypes of the four rice parents; (2) hybridizing the four rice parents to obtain F2-generation population restorer line gene molecular-marker-assisted selection results, and subjecting F2-genernation segregation population to molecular-marker-assisted selection to obtain a restorer line lane; (3) subjecting F4 generation to molecular-marker-assisted selection. More restore lines under wide restorer type range can be attained withina short time by molecular-marker-assisted selection; manpower, material and financial resources can be saved.

The invention relates to the technical field of agricultural production, and provides a culture medium and a tissue culture method for tissue culture of Craterostigma pumilum Hochst. The culture medium for tissue culture of the Craterostigma pumilum Hochst comprises a seedling breeding culture medium, a cluster bud induction culture medium, a proliferation culture medium and a rooting culture medium, wherein all the media are based on cheap and easily available MS culture media and are prepared by adding appropriate exogenous hormones or raw materials. The formula of each medium is scientific and reasonable, the media can be correspondingly used for each stage of tissue culture of the Craterostigma pumilum Hochst, the culture process is high in propagation speed, short in period and high in plant survival rate which can reach about 90%, the excellent hereditary character of the Craterostigma pumilum Hochst variety can be well kept, and market popularization and garden application of the variety are favorably promoted.

The invention discloses a multi-agent job shop negotiation scheduling optimization method. The method is divided into two stages: in the first stage, a scheme set is guided to evolve to a Pareto frontier in an inter-agent iterative negotiation mode; and in the second stage, the coordinator selects the scheme with the best social benefit from the scheme set based on the agent score. According to the method, each agent selects a part of random search schemes to put forward and form an initial parent scheme set; the coordinator performs crossover variation on the parent scheme set to generate a child scheme set, then ranks the parent and child scheme sets based on agents, and selects non-dominated schemes from the parent and child scheme sets to enter a new parent scheme set; and the negotiation is iterated for multiple times, and after a negotiation round is reached, the coordinator selects the scheme with the best social benefit from the scheme set based on the agent score. The method is based on the genetic evolution thought, and the optimization effect is effectively improved; and a more accurate information batch exposure mode is adopted, so that the social benefit is effectivelyimproved.